Effect and mechanism of Bushen Zhuyun Decoction in treatment of model rats with luteal phase defect

LIU Yu-nan; LIU Bei; YUAN Yi-zhen; ZHANG Shuang; ZHOU Hui-fang

doi:10.16025/j.1674-1307.2022.09.007

您当前的位置：

首页 >

文章列表页 >

Effect and mechanism of Bushen Zhuyun Decoction in treatment of model rats with luteal phase defect

更新时间：2023-06-16

- Effect and mechanism of Bushen Zhuyun Decoction in treatment of model rats with luteal phase defect
- Beijing Journal of Traditional Chinese Medicine Vol. 41, Issue 9, Pages: 973-977(2022)
- 作者机构：
  
  1.河南中医药大学第一临床医学院，郑州 450046
  2.南京中医药大学第一临床医学院，南京 210023
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2022.09.007
  CLC：
扫描看全文
刘玉楠，刘贝，袁艺祯，等.补肾助孕方对黄体功能不全模型大鼠的治疗作用及机制［J］.北京中医药，2022，41（9）：973-977.

LIU Yu-nan,LIU Bei,YUAN Yi-zhen,et al.Effect and mechanism of Bushen Zhuyun Decoction in treatment of model rats with luteal phase defect[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(09):973-977.
刘玉楠，刘贝，袁艺祯，等.补肾助孕方对黄体功能不全模型大鼠的治疗作用及机制［J］.北京中医药，2022，41（9）：973-977. DOI： 10.16025/j.1674-1307.2022.09.007.

LIU Yu-nan,LIU Bei,YUAN Yi-zhen,et al.Effect and mechanism of Bushen Zhuyun Decoction in treatment of model rats with luteal phase defect[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(09):973-977. DOI： 10.16025/j.1674-1307.2022.09.007.

摘要

目的,2,探讨补肾助孕方对黄体功能不全（luteal phase defect，LPD）模型大鼠的治疗作用及机制。,方法,2,将30只SD雌性大鼠随机分为空白、模型、中药低剂量、中药高剂量、阳性对照组。观察大鼠进入动情期后，上午除空白组外各组大鼠灌服米非司酮，空白组灌服生理盐水，下午各用药组灌服相应的药物，空白、模型组灌服生理盐水，连续3个动情周期，完成LPD的造模及给药后处死大鼠。检测大鼠血清雌二醇（E,2,）、孕酮（P）水平，KNDy神经元上3种神经肽及其受体（Kisspeptin/GPR54、NKB/NK3R、DYN/KOR）的mRNA与蛋白水平。,结果,2,与空白组比较，模型组E,2,、P均有下降趋势，但差异无统计学意义（,P,>,0.05）；与模型组相比，中药低、高剂量组E,2,、P均有上升趋势，其中中药低、高剂量组E,2,差异均有统计学（,P,<,0.05）。与空白组比较，模型组KNDy神经元相关肽的mRNA水平均有下降趋势，其中GPR54、NK3R、DYN差异有统计学意义（,P,<,0.05）；与模型组比较，中药低剂量、高剂量组相关基因均有上升趋势，其中中药低、高剂量组GPR54、NK3R、DYN差异均有统计学意义（,P,<,0.05）。与空白组比较，模型组KNDy神经元相关肽的蛋白表达均有下降趋势，其中NKB、DYN、KOR差异有统计学意义（,P,<,0.05）；与模型组相比，中药低剂量、高剂量组KNDy神经元相关蛋白均有上升趋势，其中中药高剂量组GPR54、NKB、NK3R、DYN、KOR差异有统计学意义（,P,<,0.05）。,结论,2,补肾助孕方可通过上调大鼠KNDy神经元相关神经肽的表达，改善卵巢黄体功能，对LPD起到治疗作用。

Abstract

Objective,2,To explore the therapeutic effect and mechanism of Bushen Zhuyun Decoction（BSZY-D）in the treatment of model rats with luteal phase defect（LPD）.,Methods,2,A total of thirty female SD rats were randomly divided into control group，model group，BSZY-D（0.9 g/100 g）group，BSZY-D（1.8 g/100 g）group and dydrogesterone group.After the rats entered the estrous period，all groups were given mifepristone in the morning except the control group，and normal saline in the control group，corresponding drugs in the afternoon，and normal saline in the control and model groups.The rats were killed after LPD modeling and administration for 3 consecutive estrous cycles.The levels of serum estradiol（E,2,）and progesterone（P），and the mRNA as well as the protein levels of the three neuropeptides and their receptors（Kisspeptin/GPR54，NKB/NK3R and DYN/KOR）were detected.,Results,2,Compared with the control group，the levels of serum E,2, and P in the model group were decreased，but the difference was not statistically significant（,P,>,0.05）；compared with the model group，the levels of E2 and P in BSZY-D（0.9g/100g）group and BSZY-D（1.8 g/100 g）group were increased，and there was significant difference in BSZY-D（0.9 g/100 g）group，BSZY-D（1.8 g/100 g）group with E,2,（,P,<,0.05）.The mRNA levels of KNDy neurons related peptide in the model group were decreased compared with the control group，the difference of GPR54，NK3R and DYN were statistically significant（,P,<,0.05）；compared with the model group，the related genes in BSZY-D（0.9 g/100 g）group and BSZY-D（1.8 g/100 g）group showed an upward trend，and there was significant difference in GPR54，NK3R，and DYN in BSZY-D（0.9 g/100 g）group and BSZY-D（1.8 g/100 g）group（,P,<,0.05）.The protein expression of KNDy neurons related peptide in model group was decreased compared with control group，and the differences of NKB，DYN and KOR were statistically significant（,P,<,0.05）；compared with the model group，the KNDy neuron related protein in BSZY-D（0.9 g/100 g）group and BSZY-D（1.8 g/100 g）group showed an upward trend，and there was significant difference in GPR54，NKB，NK3R，DYN and KOR in BSZY-D（1.8 g/100 g）group（,P,<,0.05）.,Conclusion,2,Bushen Zhuyun Decoction might improve ovarian luteal endocrine by up regulating the expression of KNDy neurons related neuropeptide in rats，so as to have a therapeutic effect on LPD.

关键词

黄体功能不全补肾助孕方KNDy神经元大鼠

Keywords

Luteal phase defectBushen Zhuyun DecoctionKNDy neuronsrats

references

艾继辉,朱桂金.黄体功能不全的诊断及对策[J].中国实用妇科与产科杂志,2010,26(10):748-750.

罗丽兰.不孕与不育[M].北京:人民卫生出版社,1998:350-358.

王琼,周灿权.黄体缺陷与黄体支持[M].中国实用妇科与产科杂志,2013,29(9):713-715.

MOORE AM,COOLEN LM,PORTER DT,et al.KNDy cells revisited[J].Endocrinology,2018,159:3219-3234.

邱雅琳,刘音吟,周惠芳.黄体功能不全性不孕症临证思路撷要[J].北京中医药,2019,38(7):665-667.

陈玮玮.补肾助孕方对黄体功能不全性不孕患者卵巢、子宫动脉血流阻力及相关因素的影响[D].南京:南京中医药大学,2011.

周伯如.补肾助孕方对在克罗米芬干预下黄体功能不全性不孕症临床疗效观察[D].南京:南京中医药大学,2014.

周惠芳,蒋凤荣,顿文亮,等.补肾助孕方对大鼠胚胎着床期子宫内膜及卵巢形态学的影响[J].陕西中医,2009,30(1):117-118.

周伯如,周惠芳,刘贝,等.补肾助孕方对LPD模型大鼠垂体GnRH受体信号转导系统的影响[J].南京中医药大学学报,2017,33(06):554-559.

孙光娟,蒋凤荣,周伯如,等.补肾助孕方药对大鼠子宫内膜细胞MMP-9mRNA、Caspase-3mRNA及细胞上清液MMP-9,TIMP-1水平表达的影响[J].时珍国医国药,2014,25(7):1780-1783.

闫丽.大鼠动情周期细胞定量方法学的建立及在毒理学研究中的应用[D].合肥：安徽医科大学,2020.

周伯如,戴佳轩,徐建亚，等.排卵后给予米非司酮对雌性大鼠动情周期、黄体功能的影响及意义[J].山东医药,2017,57(44):32-35.

KNOBIL E.The neuroendocrine control of the menstrual cycle[J].Recent Prog Horm Res,1980,36:53-88.

王新,谭建华,赖小平,等.LH在体外对GnRH脉冲模式的应答[J].中国农学通报,2011,27(3):365-368.

YU B,RUMAN J,CHRISTMAN G.The role of peripheral gonadotropin-releasing hormone receptors in female reproduction[J].Fertil Steril,2011,95(2):465-473.

BURKE MC,LETTS PA,KRAJEWSKI SJ,et al.Coexpression of dynorphin and neurokinin B immunoreactivity in the rat hypothalamus:morphologic evidence of interrelated function within the arcuate nucleus[J].J Comp Neurol,2006,498:712-726.

WAKABAYASHI Y,NAKADA T,MURATA K,et al. Neurokinin B and dynorphin A in kisspeptin neurons of the arcuate nucleus participate in generation of periodic oscillation of neural activity driving pulsatile gonadotropin-releasing hormone secretion in the goat[J].J Neurosci,2010,30:3124-3132.

王鑫焱,许小凤.从心-肾-子宫轴论治滑胎[J].中华中医药杂志,2020,35(1):209-211.

王静.夏桂成教授从心论治闭经的学术思想探讨[J].南京中医药大学学报,2015,31(5):401-406.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on mechanism of protective effect in nonalcoholic fatty liver rats by TiaoganQuzhi Formula based on miR-34a/SIRT1/PPARα signaling pathway

Research on the regulation and mechanism of Buzhong Yiqi Decoction for immune imbalance in experimental autoimmune thyroiditis rats

Mechanism of Rhizoma coptidis in improving diarrhea in ulcerative colitis through regulating the expression of AQPs protein in colonic mucosal epithelial cells

Effect of Jianpi Liqi Decoction on the improvement of micro-inflammation in the duodenum of rats with functional dyspepsia

Efficacy of Naoan Dropping Pill on the intervention of migraine under fatigue state and the mechanism of oxidative stress based on Nrf2/ARE pathway

Related Author

No data

Related Institution

Beijing Institute of Traditional Chinese Medicine

Digestive Center of Beijing Traditional Chinese Medicine Hospital Affiliated to the Capital Medical University

Beijing University of Traditional Chinese Medicine

Beijing University of Chinese Medicine

Department of Chinese Medicine Surgery，China-Japan Friendship Hospital

⁰