Objective,2,An UPLC-MS/MS method was established to study the effects of different origin， harvest period， tree age and processing methods on caffeine content in Ginkgo Folium.,Methods,2,The sample was extracted by ultrasound with methanol，steamed drying solvent，alkalized after dissolution of acid water and chloroform extraction 3 times，and separated by ACQUITY UPLC HSS T3 chromatographic column，with mobile phase of 0.1% formic acid solution-0.1% formic acid acetonitrile solution for gradient elution.Data were conducted at ESI+ multiple reaction monitoring（MRM）mode；quantitative analysis was conducted with external standard method.And the effects of origin，harvesting period，tree age and processing method on caffeine content in Ginkgo Folium was studied by statistics.,Results,2,With caffeine in the range of 0.06 ng/mL~ 62.5 ng/mL，there was a good linear relationship between the mass concentration of caffeine and the peak area，,R,2,=0.9999；the sample recovery rate was 84.94%，with RSD of 7.99%.The content range of caffeine in 77 batches of Ginkgo Folium sample was 0.1585~22.1894 μg/kg.The caffeine content of Ginkgo Folium produced in Ningqiang，Shaanxi province and Changting，Fujian province，was highest，second in Pizhou，Jiangsu Province，Tancheng，Shandong Province and Dandong，Liaoning Province，and lowest in Wenshan，Yunnan Province.,Conclusion,2,The content of caffeine in most Ginkgo Folium is stable at a lower level（below 10 μg/kg）.The content of caffeine in Ginkgo Folium is not significantly related with the tree age，harvesting period and processing method，but with the origin of Ginkgo Folium.