Effects of <italic style="font-style: italic">Lidan Xiaoshi Mixture</italic> on the intestinal microbiota of guinea pigs with cholelithiasis based on 16S rDNA sequencing technology

LYU Fuqi; BIAN Haoyu; WEI Jiaoyang; ZHANG Liping

doi:10.16025/j.1674-1307.2025.10.010

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Effects of Lidan Xiaoshi Mixture on the intestinal microbiota of guinea pigs with cholelithiasis based on 16S rDNA sequencing technology

更新时间：2025-12-15

- Effects of Lidan Xiaoshi Mixture on the intestinal microbiota of guinea pigs with cholelithiasis based on 16S rDNA sequencing technology
- Beijing Journal of Traditional Chinese Medicine Vol. 44, Issue 10, Pages: 1275-1284(2025)
- 作者机构：
  
  1.北京中医药大学东方医院，北京 100078
  2.北京市丰台区中医医院呼吸脾胃病科，北京 100076
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2025.10.010
  CLC：
- Received：21 February 2025，
  
  Published：25 October 2025
- 稿件说明：
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吕福琪，卞昊宇，魏骄阳，等.基于16S rDNA测序技术研究利胆消石合剂对胆石症豚鼠模型肠道菌群的影响［J］.北京中医药，2025，44（10）：1275-1284.

LYU Fuqi,BIAN Haoyu,WEI Jiaoyang,et al.Effects of Lidan Xiaoshi Mixture on the intestinal microbiota of guinea pigs with cholelithiasis based on 16S rDNA sequencing technology[J]. Beijing Journal of Traditional Chinese Medicine,2025,44(10):1275-1284.
吕福琪，卞昊宇，魏骄阳，等.基于16S rDNA测序技术研究利胆消石合剂对胆石症豚鼠模型肠道菌群的影响［J］.北京中医药，2025，44（10）：1275-1284. DOI： 10.16025/j.1674-1307.2025.10.010.

LYU Fuqi,BIAN Haoyu,WEI Jiaoyang,et al.Effects of Lidan Xiaoshi Mixture on the intestinal microbiota of guinea pigs with cholelithiasis based on 16S rDNA sequencing technology[J]. Beijing Journal of Traditional Chinese Medicine,2025,44(10):1275-1284. DOI： 10.16025/j.1674-1307.2025.10.010.

摘要

目的

使用16S rDNA测序技术分析利胆消石合剂对胆石症豚鼠肠道菌群的影响。

方法

用随机数字表法将豚鼠分为对照组（CG）10只，模型组（MG）、胆宁片（DN）组、中药低剂量（ZD）组、中药中剂量（ZZ）组、中药高剂量（ZG）组各15只。除CG组外，其他各组采用胆道插管、注射复发型胆总管结石患者胆道细菌培养液的方式制备胆道结石复发模型。造模结束后次日，DN组给予0.05 g/（100 g·d）胆宁片灌胃，ZD、ZZ、ZG组分别给予0.16、0.32、0.64 g/（100 g·d）利胆消石合剂颗粒灌胃，CG、MG给予等体积蒸馏水灌胃，均持续7 d。采用16S rDNA技术检测各组豚鼠肠道菌群的组成，分析肠道菌群的组成结构及丰度变化。

结果

造模方式显著改变了肠道菌群的组成结构，MG与其他各组的肠道菌群物种组成差异有统计学意义（

0.05）；利胆消石合剂各干预组改变了造模导致的物种组成结构。并且在属水平6组间共有12种菌属在群落丰度上差异有统计学意义（

0.05）。

结论

利胆消石合剂可能通过调节肠道菌群的结构组成，影响“胆汁酸-肠道菌群”平衡及胆汁酸的“肠肝循环”，从而影响胆总管结石的复发。

Abstract

Objective

To investigate the effects of

Lidan Xiaoshi Mixture

on the intestinal microbiota of guinea pigs with cholelithiasis using 16S rDNA sequencing technology.

Methods

Guinea pigs were randomly assigned by random number table method into six groups： control group （CG，

=10）， model group （MG，

=15）， Danning Tablets group （DN，

=15）， low-dose

Lidan Xiaoshi Mixture

group （ZD，

=15）， medium-dose

Lidan Xiaoshi Mixture

group （ZZ，

=15）， and high-dose

Lidan Xiaoshi Mixture

group （ZG，

=15）. Except for CG， the other groups were subjected to biliary tract intubation and injection of biliary bacterial culture solution from patients with recurrent common bile duct stones to establish a recurrent biliary calculi model. On the day following model establishment， the DN group received

Danning Tablets

at 0.05 g/（100 g·d） by gavage， while the ZD， ZZ， and ZG groups received

Lidan Xiaoshi Mixture

at 0.16， 0.32， and 0.64 g/（100 g·d）， respectively. CG and MG received equal volumes of distilled water. All treatments were administered for 7 days. The intestinal microbiota composition of each group was analyzed using 16S rDNA sequencing， and changes in microbial composition and abundance were evaluated.

Results

The modeling procedure significantly altered the intestinal microbiota composition， with the MG group showing statistically significant differences in species composition compared with other groups （

0.05）. Intervention with

Lidan Xiaoshi Mixture

restored the species composition disrupted by modeling. At the genus level， 12 bacterial genera showed significant differences in community abundance among the six groups （

0.05）.

Conclusion

Lidan Xiaoshi Mixture

may modulate the structural composition of the intestinal microbiota， influencing the balance of the "bile acid-intestinal microbiota" axis and the enterohepatic circulation of bile acids， thereby affecting the recurrence of common bile duct stones.

关键词

Keywords

references

YAO W , JING CX , FU SX . Advances in risk factors for recurrence of common bile duct stones [J ] . Int J Med Sci , 2021 , 18 ( 4 ): 1067 - 1074 .

LIVIA A , ALBERTO M , GABRIELE C , et al . Needle-knife fistulotomy vs.standard biliary sphincterotomy for choledocholithiasis:common bile duct stone recurrence and complication rate [J ] . Endosc Int Open , 2019 , 7 ( 12 ): E1733 - E1741 .

王惠临 , 张立平 , 卞昊宇 , 等 . 利胆消石合剂对胆总管结石患者ERCP术后胆汁成分影响的临床研究 [J ] . 天津中医药大学学报 , 2023 , 42 ( 3 ): 280 - 285 .

卞昊宇 , 韩冉 , 段学光 , 等 . 运用复发型胆总管结石患者胆道细菌建立豚鼠胆石症模型 [J ] . 河北医学 , 2024 , 30 ( 1 ): 40 - 45 .

SHIMIZU M , MORIMOTO H , MARUYAMA R , et al . Selective regulation of FGF19 and FGF21 expression by cellular and nutritional stress [J ] . J Nutr Sci Vitaminol (Tokyo) , 2015 , 61 ( 2 ): 154 - 160 .

干丹 , 杨豪杰 , 黄冕 , 等 . 胆汁酸代谢及其受体调节溃疡性结肠炎肠道免疫及中药干预研究进展 [J ] . 上海中医药杂志 , 2023 , 57 ( 9 ): 96 - 100 .

VERENA K , DIETER H . Role of TGR5 (GPBAR1) in liver disease [J ] . Semin Liver Dis , 2018 , 38 ( 4 ): 333 - 339 .

叶绿望 , 王丛 , 范俊维 , 等 . 基于FXR-FGF19通路研究益生菌对胆总管结石患者胆汁酸代谢的影响 [J ] . 胃肠病学 , 2024 , 29 ( 1 ): 10 - 14 .

JIANG L , ZHANG H , XIAO D , et al . Farnesoid X receptor (FXR): Structures and ligands [J ] . Comput Struct Biotechnol J , 2021 （ 19 ）: 2148 - 2159 .

高雷 , 朱英 , 白长川 , 等 . 中医药通过肠道菌群影响胆汁酸代谢治疗脂肪肝的研究进展 [J ] . 中国医药科学 , 2024 , 14 ( 13 ): 16 - 19，48 .

李萍 , 杨喜乐 , 朱安琪 , 等 . 从胆汁酸-肠道菌群轴失调探讨疏肝理脾方对腹泻型肠易激综合征肝郁脾虚证患者的影响 [J ] . 广州中医药大学学报 , 2025 , 42 ( 1 ): 94 - 100 .

王敏 , 周璐 , 孙燕 , 等 . 大柴胡汤及其“方剂要素”对NAFLD模型大鼠“肠-肝轴”作用的相关分析 [J ] . 世界中医药 , 2021 , 16 ( 3 ): 430 - 436 .

边甜甜 , 司昕蕾 , 牛江涛 , 等 . 脾气虚证与肠道菌群的相关性及健脾益气中药对肠道菌群的调节作用研究进展 [J ] . 中药药理与临床 , 2022 , 38 ( 5 ): 212 - 217 .

黄灿 , 沈垚村 , 刘深梵 , 等 . 基于“肝-胆汁酸-肠道菌群轴”论非酒精性脂肪性肝病肝郁脾虚病机的生物学内涵 [J ] . 北京中医药 , 2023 , 42 ( 5 ): 551 - 555 .

卞昊宇 , 吕福琪 , 魏骄阳 , 等 . 探究利胆消石合剂预防ERCP术后CBDS复发的作用机制 [J/OL ] . 辽宁中医药大学学报 , 1 - 21 [ 2025-02-09 ] . http://kns.cnki.net/kcms/detail/21.1543.R.20241121.1225.004.html http://kns.cnki.net/kcms/detail/21.1543.R.20241121.1225.004.html .

YANG R , LI X , YING Z , et al . Prematurely delivering mothers show reductions of lachnospiraceae in their gut microbiomes [J ] . BMC Microbiol , 2023 , 23 ( 1 ): 169 .

VACCA M , CELANO G , CALABRESE MF , et al . The controversial role of human gut lachnospiraceae [J ] . Microorganisms , 2020 , 8 ( 4 ): 573 .

王湘鹏 , 曾勤 , 杨嘉仪 , 等 . 余仁欢基于肠道菌群学说从脾论治特发性膜性肾病经验 [J ] . 北京中医药 , 2024 , 43 ( 4 ): 381 - 384 .

王梦依 , 汪卓 , 刘洋 . 肠道菌群调控脂质代谢作用与机制的研究进展 [J ] . 微生物学通报 , 2025 , 52 ( 3 ): 917 - 930 .

FERNANDES VR , JEFFERSON OE , ÍTALO SP , et al . Akkermansia muciniphila and gut immune system: a good friendship that attenuates inflammatory bowel disease, obesity, and diabetes [J ] . Front Immunol , 2022 ， 13 : 934695 .

MONIKA K , ANNA K , JANA S , et al . Probiotics and postbiotics in colorectal cancer: prevention and complementary therapy [J ] . World J Gastroenterol , 2022 , 28 ( 27 ): 3370 - 3382 .

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Effects of Lidan Xiaoshi Mixture on the intestinal microbiota of guinea pigs with cholelithiasis based on 16S rDNA sequencing technology

DOI：10.16025/j.1674-1307.2025.10.010

摘要

Abstract

关键词

Keywords

references