Objective To investigate the effects of Astragalus,Panax notoginseng and their compatibility on the expression of Gli1/2/3,SUFU and Cyclin D1 in precancerous lesions of gastric cancer（ PLGC） rats. Methods Male Wistar rats were randomly divided into control group,model group,Astragalus group,Panax notoginseng group,Astragalus plus Panax notoginseng group,cyclopamine group,Purmorphamine group and folic acid group of 10 each. PLGC model was established by MNNG and treated with the corresponding drugs for 8 weeks. Then,RT-PCR and Western blot were used to observe the expression of Gli1/2/3 mRNA and gene level. Immunofluorescence was used to detect the protein expression of SUFU and Cyclin D1. Results Compared with the control group,Gli-1 mRNA was reduced in the model group（ P < 0. 01）,the protein expression of Gli-2 and Gli-3 were obviously up-regulated（ P <0. 01）; the expression of SUFU protein was strengthened and Cyclin D1 was weakened（ P < 0. 01）. Compared with the model group,Panax notoginseng could increase the expression of Gli-1 mRNA（ P < 0. 05） and reduce the expression of Gli-2/3 and SUFU protein（ P < 0. 05,P < 0. 01）. The compatibility group could improve the expression of Gli-3 and SUFU（ P < 0. 05,P < 0. 01）,while Astragalus group could only reduce SUFU protein expression（ P < 0. 01） and showed no obvious effects on other indexes. Conclusion Panax notoginseng for activating blood circulation can play a role in preventing PLGC by regulating the expression of key factors in hedgehog signaling pathway of stomach mucous of rats with precancerous lesions of gastric cancer（ PLGC） so as to prevent precancerous lesions of gastric cancer.