Objective To observe the curative effects of method for adjusting knee joint in a sitting position on knee osteoarthritis（KOA）and the influence on knee function and walking ability of patients. Methods A total of 76 cases of KOA patients were randomly divided into two groups,the control group of 38 patients were treated with traditional tendon-regulating massage therapy,and the observation group of 38 patients with the therapy of adjusting knee joint in a sitting position. The symptoms,data and clinical effects of two groups before and after the treatment were compared. Results The immediate effective rate of the observation group and the control group were 80. 00% and 71. 05%respectively,and the difference was statistically significant（P < 0. 05）. After the treatment,the total scores of WOMAC,pain,stiffness,and the degree of daily activity of both groups were much more improved than that before the treatment（P < 0. 05）. The difference of immediate pain scores of two groups were statistically significant（P < 0. 05）. After four weeks of treatment,the differences of gait parameters between the two groups,including step length,step size,swing phase,double support phase,were also statistically significant（P < 0. 05）.Conclusion The method for adjusting knee joint in a sitting position has a better immediate effect than that of the traditional massage therapy for it can improve pain obviously; and even improve walking ability after four weeks of treatment.