Study on simultaneous determination of seven effective components in Ligusticum chuanxiong Hort.-Cyperus rotundus L.

GUO Li; ZHAI Yong-song; WANG Min; XU Yong-song; GONG Mu-xin; WU Sha

doi:10.16025/j.1674-1307.2018.03.010

您当前的位置：

首页 >

文章列表页 >

Study on simultaneous determination of seven effective components in Ligusticum chuanxiong Hort.-Cyperus rotundus L.

更新时间：2023-04-10

- Study on simultaneous determination of seven effective components in Ligusticum chuanxiong Hort.-Cyperus rotundus L.
- Beijing Journal of Traditional Chinese Medicine Vol. 37, Issue 3, Pages: 225-229(2018)
- 作者机构：
  
  1. 首都医科大学中医药学院中医络病研究北京市重点实验室
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2018.03.010
  CLC：
扫描看全文
GUO Li, ZHAI Yong-song, WANG Min, et al. Study on simultaneous determination of seven effective components in Ligusticum chuanxiong Hort.-Cyperus rotundus L.. [J]. Beijing Journal of Traditional Chinese Medicine 37(3):225-229(2018)
DOI：

GUO Li, ZHAI Yong-song, WANG Min, et al. Study on simultaneous determination of seven effective components in Ligusticum chuanxiong Hort.-Cyperus rotundus L.. [J]. Beijing Journal of Traditional Chinese Medicine 37(3):225-229(2018) DOI： 10.16025/j.1674-1307.2018.03.010.

摘要

目的建立同时测定川芎-醋香附药对中阿魏酸、洋川芎内酯I、洋川芎内酯H、洋川芎内酯A、Z-藁本内酯、丁烯基苯酞及α-香附酮7种有效成分的高效液相色谱（HPLC）分析方法。方法采用Kromasil-C18色谱柱（4.6 mm×250 mm,5μm）,流动相甲醇（A）-0.1%甲酸水溶液（B）梯度洗脱,柱温25℃,检测波长分别为250 nm和286 nm,流速1.0 m L/min。结果 7种有效成分的线性关系良好;各成分平均回收率分别为99.7%、99.6%、98.9%、101.2%、99.4%、100.3%、99.0%,RSD分别为1.7%、1.5%、1.3%、2.3%、2.2%、1.6%、2.1%;其余方法学考察结果符合要求。结论该方法简便、快速、准确,可用于川芎-醋香附药对中7种有效成分的同步测定,同时可为川芎-醋香附药对全面质量评价和质量控制提供科学依据。

Abstract

Objective To develop a HPLC method for simultaneous determination of ferulic acid,Senkyunolide I,Senkyunolide H,Senkyunolide A,z-ligustilide,Butylidenephthalide and α-Cyperone in Ligusticum chuanxiong Hort.-Cyperus rotundus L.. Methods The HPLC system consisted of a Kromasil-C18 column（ 4. 6 mm × 250 mm,5 μm）,with the mobile phase of methanol solution（ A）-water solution containing 0. 1% formic acid（ B） for gradient elution. DAD detector was used and the detection wave lengths were 250 nm and286 nm. The flow rate was 1. 0 m L/min and the column temperature was 25 ℃. Results The liner relationships of seven effective components were favorable; the mean rate of recovery of each were 99. 7%,99. 6%,98. 9%,101. 2%,99. 4%,100. 3%,99. 0%,and RSD were 1. 7%,1. 5%,1. 3%,2. 3%,2. 2%,1. 6%,2. 1%; other methodological investigation results followed the requirements. Conclusion This method is simple,rapid and correct,and it can be used for simultaneous determination of seven effective components in Ligusticum chuanxiong Hort.-Cyperus rotundus L.,and meanwhile,provide scientific evidences for overall quality evaluation and control of it.

关键词

高效液相色谱川芎-醋香附有效成分质量控制

Keywords

references

Views

319

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Clinical and experimental research progress of Arnebia euchroma preparation for external use in dermatology

Study on preparation and quality control of Qinbai Film Agent

Related Author

No data

Related Institution

天津中医药大学第一附属医院

首都医科大学附属北京中医医院临床药学科

⁰