Objective To explore and screen out the biochemical indicators in relationship with the indexes of TCM deficiency and excess attributes in patients with acute-on-chronic acute-on-chronic liver failure（HBV-ACLF）,and evaluate their diagnostic value for predicting development of TCM deficiency and excess in order to provide assistance in making syndrome differentiation of TCM.Methods 130 patients with HBV-ACLF were included,and according to TCM syndrome differentiation criteria,they were divided into:blood stasis-heat jaundice,damp-heat jaundice,Qi-deficiency stasis jaundice,Yin-deficiency stasis jaundice and Yang-deficiency stasis jaundice.Blood stasis-heat jaundice and damp-heat jaundice were classified into excessive syndrome group with 90 cases.Qi-deficiency stasis jaundice,Yin-deficiency stasis jaundice and Yang-deficiency stasis jaundice were classified into deficiency syndrome group of 40 cases.Those index,including ALT,AST,TBiL,ALB,γ-GT,ALP,PA,CREA,PTA,INR,TC,TG,HDL-C,LDL-C,AFP,AFU,WBC,Hb and PLT Etc were screened out.The excess syndrome were taken as the positive trend prediction,the biochemical indicators of P<0.100 were included in the binomial logistic regression model for independent prediction analysis,and the threshold value,sensitivity and specificity of the prediction index were determined by the area under the receiver’s working curve（AUROC）.Results The indicators,such as AFU,PA,HDL-C and LDL-C excessive syndrome groups were higher than those of deficiency syndrome groups and the differences were statistically significant（P<0.05）.The rest indicators were not statistically significant（P>0.05）.To compare the excessive syndrome groups with the deficiency syndrome groups,the excessive syndrome trend prediction was used to compare the biochemical indicators including AFU,γ-GT,INR,PA,TC,TG,HDL-C,LDL-C and Hb of P<0.100 in Chinese medicine to the binomial logistic regression model.Indicators such as AFU,PA,and HDL-C were the excessive syndrome independent predictors.The empirical AUROC value of AFU level prediction was 0.706（95%CI 0.583～0.829）,and the threshold value was 39.20（U/L）.Above the threshold value,it was likely to be the excessive syndrome,with sensitivity of 78.20% and specificity of 47.60 %;PA level prediction the excessive syndrome AUROC value was 0.706（95%CI 0.587～0.826）,the threshold value was 37.60（mg/L）,above the threshold value was likely to be the excessive syndrome,the predicted trend sensitivity was 74.50%,and specificity 42.90%;the excessive syndrome AUROC value of HDL-C level was 0.711（95%CI 0.597～0.826）,and the threshold value was 0.36（mmol/L）.The fact that was above the threshold value suggest possibility of excessive syndrome,and the predicted trend sensitivity was 65.50 %,specificity was 38.10%.Conclusion AFU,PA and HDL-C can predict deficiency and excess syndromes in TCM with better sensitivity but less specificity,there is also a certain false positive,and the sample needs to be expanded.Multi-centered joint research can further screen out objective indicators that are more sensitive to predict TCM deficiency and excess attributes.