Objective To investigate the effect of Qinggan Huatan Huoxue Formula on fatty deposition in liver cell of rats with non-alcoholic fatty liver disease（NAFLD） and regulation of sterol regulatory element binding protein-1 c.Methods 60 SPF-class male SD rats were selected and divided into normal group,model group,treatment group with 20 in each group.Apart from the normal group,NAFLD rat model was replicated with high fat diet in other groups.After 8 weeks of modeling,10 rats were taken from each group randomly.After verifying the success of modeling,the treatment group was treated with Qinggan Huatan Huoxue Formula,and the model group and normal group were given saline.Serum ALT,AST,γ-GT,and TC,TG and FFA in liver tissue were tested to observe fatty degeneration in liver.The expression of SREBP-1 c mRNA was detected by real time quantitative PCR method.Results After 4 weeks of treatment,the liver tissue of normal group was normal.In the model group,fatty degeneration occurred in different degrees.Compared with the normal group,serum ALT,AST,and R-GGT in the model group were increased significantly with significant differences（P<0.05）;Compared with the model group,serum ALT,AST,γ-GT activity were decreased significantly in the treatment group（P< 0.05）;the levels of TC,TG,and FFA in liver tissue,were significantly lowered in the treatment group than that in the model group（P<0.01）;compared with the normal group,the expression of SREBP-1 c gene in hepatocytes in the model group was increased significantly（P<0.01）;compared with the model group,the level of expression in the treatment group was significantly reduced（P<0.01）.Conclusion Qinggan Huatan Huoxue Formula can improve liver inflammation by controlling liver cell SREBP-1 c to make it recover from fatty deposition in liver cells of rats with NAFLD.