Comparativestudy on pharmacological effects of Shenma Yizhi Formula processed with three kinds of preparation techniques

LI Nan-nan; LIU Jian-gang; ZHENG Rui; LI Hao; LIU Mei-xia; LUO Zeng-gang

doi:10.16025/j.1674-1307.2019.05.006

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Comparativestudy on pharmacological effects of Shenma Yizhi Formula processed with three kinds of preparation techniques

更新时间：2023-04-10

- Comparativestudy on pharmacological effects of Shenma Yizhi Formula processed with three kinds of preparation techniques
- Beijing Journal of Traditional Chinese Medicine Vol. 38, Issue 5, Pages: 427-432(2019)
- 作者机构：
  
  1. 中国中医科学院西苑医院
  2. 北京市中医管理局
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2019.05.006
  CLC：
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LI Nan-nan, LIU Jian-gang, ZHENG Rui, et al. Comparativestudy on pharmacological effects of Shenma Yizhi Formula processed with three kinds of preparation techniques. [J]. Beijing Journal of Traditional Chinese Medicine 38(5):427-432(2019)
DOI：

LI Nan-nan, LIU Jian-gang, ZHENG Rui, et al. Comparativestudy on pharmacological effects of Shenma Yizhi Formula processed with three kinds of preparation techniques. [J]. Beijing Journal of Traditional Chinese Medicine 38(5):427-432(2019) DOI： 10.16025/j.1674-1307.2019.05.006.

摘要

目的比较3种提取工艺制备的参麻益智方对山莨菪碱所致血管性痴呆模型大鼠学习记忆能力、脑组织病理形态及血脂、氧化应激、炎症因子、胆碱能系统等主要药效学指标的药理作用。方法清洁级SD大鼠60只,随机分为6组,每组10只。3种不同工艺制备的参麻益智方组,分别给予参麻益智方1号方、2号方和3号方2.97 g/（kg·d）;阳性对照药组给予盐酸多奈哌齐片0.45 mg/（kg·d）;空白组和模型组均给予等体积纯净水。给药4周末,除空白组外,其他各组分别注射盐酸消旋山莨菪碱注射液2 mg/kg。水迷宫测试大鼠学习记忆能力,腹腔麻醉后取脑组织检测海马CA1区病理形态,取血清检测血脂四项及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、丙二醛（MDA）、白介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、一氧化氮（NO）、乙酰胆碱（Ach）、乙酰胆碱酯酶（AchE）等主要药效学指标。结果与模型组比较,参麻益智方3组均可显著降低大鼠总胆固醇（TC）水平（P<0.05）;1号方组大鼠SOD活性及NO水平显著升高（P<0.05）。与2号方组比较,3号方组大鼠的穿台次数增加显著（P<0.05）;与2号方组、3号方组比较,1号方组大鼠的SOD活性显著升高（P<0.05）;与3号方组比较,1号方组、2号方组大鼠的TNF-α水平降低、NO水平升高（P<0.05）。结论 3种不同工艺制备的参麻益智方对血管性痴呆大鼠相关药效学的作用环节有一定差异,参麻益智方1号方的药效优于2号方和3号方。

Abstract

Objective To compare the pharmacological effects of Shenma Yizhi Formula processed with 3 kinds of preparation techniques on learning and memory capability, brain histopathology, blood lipid indexes, oxidative stress indexes, inflammatory factors, cholinergic indexes in anisodamine-induced model rats.Methods 60 SD rats, 30 female and 30 male, were randomly divided into 6 groups with 10 rats in each group.3 Shenma Yizhi Formula groups with 3 different preparation techniques, including No.1 recipe group, No.2 recipe and No.3 recipe, were given the formula with 2.97 g·kg,-1,·d,-1,;the positive control group, donepezil hydrochloride tablet, with 0.45 mg·kg,-1,·d,-1,;the blank group and the model group, the equal amounts of pure water.After 4 weeks of delivery, the racemic anisodamine hydrochloride（2 mg/kg）was intraperitoneal injected separately to all the rats except for the blank group.The learning and memory ability of all rats was tested by Morris water maze.All rats were anesthetized by chloral hydrate.Serum indexes of lipids, superoxide dismutasee（SOD）, glutathione peroxidase（GSH-PX）, malondialdehyde（MDA）, interleukin-1β（IL-1β）, tumor necrosis factor-α（TNF-α）, nitric oxide（NO）, acetylcho1（Ach）and acetylcholinesterase（AchE）were tested, and the pathological morphology of hippocampal CA1 region observed.Results Compared with the model group, TC level of all Shenma Yizhi Formula groups were decreased（P,<,0.05）.SOD activity and NO level of rats in No.1 recipe group were significantly increased（P,<,0.05）.Compared with No.2 recipe group, the passing platform times of rats in No.3 recipe group were significantly increased（P,<,0.05）.Compared with No.2 recipe group and No.3 recipe group, the SOD activity of No.1 recipe group was significantly increased（P,<,0.05）.Compared with No.3 recipe group, the level of TNF-α was decreased in No.1 recipe group and No.2 recipe group, and the level of NO was increased（P,<,0.05）.Conclusion The pharmacological effects of Shenma Yizhi Formula prepared with 3 kinds of techniques are different, and the pharmacological effect indexes of No.1 recipe were better than those of No.2 recipe and No.3 recipe.

关键词

血管性痴呆参麻益智方氧化应激炎症因子行为学检测大鼠

Keywords

vascular dementiaShenma Yizhi Formulaoxidative stressinflammatory factorbehavioral testingrats

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