Improvement ofSchisandrin B for H<sub>2</sub>O<sub>2</sub> induced human retinal pigment epithelial cells injury through up-regulation of miR-374a

WANG Ying-hao; SONG Hui

doi:10.16025/j.1674-1307.2020.06.008

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Improvement ofSchisandrin B for H₂O₂ induced human retinal pigment epithelial cells injury through up-regulation of miR-374a

更新时间：2023-04-11

- Improvement ofSchisandrin B for H₂O₂ induced human retinal pigment epithelial cells injury through up-regulation of miR-374a
- Beijing Journal of Traditional Chinese Medicine Vol. 39, Issue 6, Pages: 553-559(2020)
- 作者机构：
  
  1. 恩施土家族苗族自治州中心医院眼科中心
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2020.06.008
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[1]王英豪,宋慧.五味子乙素通过上调miR-374a改善过氧化氢诱导人视网膜色素上皮细胞损伤[J].北京中医药,2020,39(06):553-559.

WANG Ying-hao, SONG Hui. Improvement ofSchisandrin B for H₂O₂ induced human retinal pigment epithelial cells injury through up-regulation of miR-374a. [J]. Beijing Journal of Traditional Chinese Medicine 39(6):553-559(2020)
[1]王英豪,宋慧.五味子乙素通过上调miR-374a改善过氧化氢诱导人视网膜色素上皮细胞损伤[J].北京中医药,2020,39(06):553-559. DOI： 10.16025/j.1674-1307.2020.06.008.

WANG Ying-hao, SONG Hui. Improvement ofSchisandrin B for H₂O₂ induced human retinal pigment epithelial cells injury through up-regulation of miR-374a. [J]. Beijing Journal of Traditional Chinese Medicine 39(6):553-559(2020) DOI： 10.16025/j.1674-1307.2020.06.008.

摘要

目的研究五味子乙素（SchB）对过氧化氢（H,2,O,2,）诱导的人视网膜色素上皮细胞（ARPE-19）氧化损伤的保护效应及其作用机制。方法 H,2,O,2,诱导ARPE-19细胞氧化损伤模型,分别采用CCK8实验、流式细胞术、DCFH-DA染色和RT-qPCR技术检测细胞活力、凋亡、ROS含量及miR-374a表达水平;Western blot方法检测、氧化应激相关蛋白表达水平,PI3K/AKT和MEK/ERK信号通路相关蛋白表达情况。结果 H,2,O,2,刺激明显降低了ARPE-19细胞活力,诱导其凋亡及氧化应激损伤;SchB改善H,2,O,2,诱导的ARPE-19细胞损伤;SchB上调H,2,O,2,诱导的ARPE-19细胞中miR-374a表达,并且miR-374a沉默部分逆转了SchB对H,2,O,2,诱导的ARPE-19细胞损伤的保护效应;SchB还可通过上调miR-374a表达降低PI3K/AKT和MEK/ERK信号通路活化。结论 SchB通过上调miR-374a表达,抑制PI3K/AKT和MEK/ERK信号传导,改善H,2,O,2,诱导的ARPE-19细胞氧化损伤;SchB可能成为治疗视网膜氧化损伤的药物。

Abstract

Objective To study the protective effects and mechanism of Schisandrin B（SchB）in the oxidative injury of human retinal pigment epithelial cells（ARPE-19）induced by H,2,O,2,.Methods ARPE-19 cells model with oxidative injury were induced by H,2,O,2,.Cell viability, apoptosis, ROS generation and miR-374 a level were measured by CCK-8 assay, flow cytometry assay, DCFH-DA staining and RT-qPCR, respectively.Expressions of proteins associated with viability, apoptosis and oxidative stress, as well as PI3 K/AKT and MEK/ERK pathways was measured by western blotting.Results H,2,O,2, reduced the cell viability obviously while it promoted apoptosis and oxidative stress in ARPE-19 cells.SchB ameliorated H,2,O,2,-induced cell injury.The expression of miR-374 a was up-regulated by SchB in H,2,O,2,-treated cells, and miR-374 a inhibition obviously reversed the effects of SchB in H,2,O,2,-treated cells.Finally, the PI3 K/AKT and MEK/ERK pathways were inhibited by SchB through up-regulation of miR-374 a.Conclusion SchB can protect ARPE-19 cells against H,2,O,2,-induced injury through up-regulation of miR-374 a and inhibition of PI3 K/AKT and MEK/ERK pathways.SchB might become a potential therapeutic drug for retinopathy.

关键词

五味子乙素人视网膜色素上皮细胞氧化应激机制

Keywords

Schisandrin Bhuman retinal pigment epithelial cellsoxidative stressmechanism

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School of Traditional Chinese Medicine， Capital Medical University

Department of Nephrology， Beijing Hospital of Traditional Chinese Medicine， Affiliated to the Capital Medical University

Beijing University of Chinese Medicine

Dongzhimen Hospital of Beijing University of Chinese Medicine

Institute of Traditional Chinese Medicine Encephalopathy，Beijing University of Chinese Medicine，Encephalopathy Department

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Improvement ofSchisandrin B for H2O2 induced human retinal pigment epithelial cells injury through up-regulation of miR-374a

DOI：10.16025/j.1674-1307.2020.06.008

摘要

Abstract

关键词

Keywords

references

Improvement ofSchisandrin B for H₂O₂ induced human retinal pigment epithelial cells injury through up-regulation of miR-374a