Effect of Shufeng Jiedu Capsules on serum T cell subsets CD3+, CD4+/CD8+ in rats with acute pharyngitis

WU Fei-hu; SONG Ren-jie; CAI Chuan-yu; QIAN Ya-qin; LIU Gang

doi:10.16025/j.1674-1307.2020.07.008

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Effect of Shufeng Jiedu Capsules on serum T cell subsets CD3⁺, CD4⁺/CD8⁺ in rats with acute pharyngitis

更新时间：2023-04-11

- Effect of Shufeng Jiedu Capsules on serum T cell subsets CD3⁺, CD4⁺/CD8⁺ in rats with acute pharyngitis
- Beijing Journal of Traditional Chinese Medicine Vol. 39, Issue 7, Pages: 689-692(2020)
- 作者机构：
  
  1. 安徽中医药大学第一附属医院耳鼻咽喉科
  2. 安徽中医药大学
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2020.07.008
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[1]吴飞虎,宋任洁,蔡传羽,钱雅琴,刘钢.疏风解毒胶囊对急性咽炎大鼠血清T细胞亚群CD3~+、CD4~+/CD8~+的影响[J].北京中医药,2020,39(07):689-692.

WU Fei-hu, SONG Ren-jie, CAI Chuan-yu, et al. Effect of Shufeng Jiedu Capsules on serum T cell subsets CD3⁺, CD4⁺/CD8⁺ in rats with acute pharyngitis. [J]. Beijing Journal of Traditional Chinese Medicine 39(7):689-692(2020)
[1]吴飞虎,宋任洁,蔡传羽,钱雅琴,刘钢.疏风解毒胶囊对急性咽炎大鼠血清T细胞亚群CD3~+、CD4~+/CD8~+的影响[J].北京中医药,2020,39(07):689-692. DOI： 10.16025/j.1674-1307.2020.07.008.

WU Fei-hu, SONG Ren-jie, CAI Chuan-yu, et al. Effect of Shufeng Jiedu Capsules on serum T cell subsets CD3⁺, CD4⁺/CD8⁺ in rats with acute pharyngitis. [J]. Beijing Journal of Traditional Chinese Medicine 39(7):689-692(2020) DOI： 10.16025/j.1674-1307.2020.07.008.

摘要

目的观察疏风解毒胶囊（ShuFeng Jiedu Capsules,SFJDC）对急性咽炎（acute pharyngitis,AP）大鼠血清T细胞亚群CD3,+,、CD4,+,/CD8,+,表达的影响。方法将70只大鼠随机分为7组,每组10只（雌雄各半）,分别为空白对照组、模型组、安慰剂组、克拉霉素缓释片组及SFJDC高、中、低剂量组。参照咽部喷雾氨水造模法制备AP大鼠模型,3 d造模成功后,第4天开始,SFJDC高、中、低剂量组分别给予SFJDC 2.2、1.1、0.55 g/（kg·d）灌胃治疗,克拉霉素缓释片组给予克拉霉素缓释片20 mg/（kg·d）灌胃治疗,安慰剂组给予等量的蒸馏水灌胃,灌胃2次/d,连续治疗5 d。观察各组大鼠一般情况,并检测血清T细胞亚群CD3,+,、CD4,+,/CD8,+,表达情况。结果大鼠行为变化及病理切片评分,模型组与空白对照组比较,差异有统计学意义（P,<,0.05）,表明AP大鼠造模成功。与安慰剂组比较,SFJDC高、中、低剂量组及克拉霉素缓释片组CD3,+,值差异无统计学意义（P,>,0.05）;CD4,+,值下降,但差异无统计学意义（P,>,0.05）;CD8,+,值升高,但仅有SFJDC高剂量组差异有统计学意义（P,<,0.01）;SFJDC高、中剂量组及克拉霉素缓释片组CD4,+,/CD8,+,比值下降,差异有统计学意义（P,<,0.05,P,<,0.01）。与克拉霉素缓释片组比较,SFJDC高剂量组CD3,+,、CD8,+,值较高,差异有统计学意义（P,<,0.05）。结论 SFJDC对AP大鼠有较好治疗作用,其机制可能是通过调控血清T细胞亚群CD3,+,、CD4,+,/CD8,+,比值而起到调节免疫的作用。

Abstract

Objective To observe the effect of Shufeng Jiedu Capsules（SFJDC）on the expression of serumCD3,+, CD4,+,/CD8,+, of rats with acute pharyngitis（AP）. Methods Seventy rats were randomly divided into seven groups, 10 rats in each group（half male and half female）, including blank control group, model group, placebo group, clarithromycin sustained-release tablet group, SFJDC high, medium and low dose groups. AP rat model was prepared with the method of pharyngeal spray and ammonia modeling. After the success of 3-day modeling, from the fourth days on, SFJDC was given intragastric administration of 2.2, 1.1 and 0.55 g/（kg·d）respectively. The clarithromycin sustained release tablets group was given clarithromycin sustained release tablets 20 mg/（kg·d）, and the placebo group was given the same amount of distilled water with intragastric administration twice a day, all continuously for 5 days. The general situation of rats in each group was observed, and the expression of CD3,+, CD4,+,/CD8,+, in serum T lymphocyte subsets was detected. Results By observing the changes of rats’ behavior and pathological sections comparison score, there was found a significant statistical difference between the model group and the blank control group（P,<,0.05）, which indicated a successful modelling of AP rats. Compared with the placebo group, there were no differences in the mean CD3,+, of the SFJDC high, medium and low dose group and the clarithromycin sustained-release tablet group（P,>,0.05）; the mean CD4,+, of the SFJDC high, medium and low dose group and clarithromycin sustained-release tablet group decreased, but the differences were not statistically significant（P,>,0.05）; the average CD8,+, of the SFJDC high, medium, and low dose groups and the clarithromycin sustained-release tablet group increased, however, only SFJDC high dose group had significant difference（P,<,0.01）; the CD4,+,/CD8,+, mean values of the SFJDC high,medium dose groups and the clarithromycin sustained-release tablet group decreased, and there were significant differences（P,<,0.05,P,<,0.01）. Compared with the clarithromycin sustained-release tablet group, the CD3,+, and CD8,+, of the SFJDC high dose group were better than the clarithromycin sustained-release tablet group, and the differences were statistically significant（P,<,0.05）.Conclusion SFJDC has a better therapeutic effect on AP rats. The mechanism of SFJDC in treating AP may be that it has immunity-regulating effect by adjusting the ratio of CD3,+, CD4,+,/CD8,+, of serum T lymphocyte subsets.

关键词

急性咽炎T细胞亚群免疫调节疏风解毒胶囊大鼠

Keywords

Acute pharyngitisT cell subsetsimmunoregulationShuFeng Jiedu Capsulesrats

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