Objective To explore the mechanism of Huiyang Shengji Ointment on inflammatory reaction of microvascular endothelial cells and improving repair of wound.Methods In vitro experiment: the cells were divided into four groups: normal group（normal endothelial cell）,model group（diseased endothelial cell）,experimental group（diseased endothelial cell + Huiyang Shengji ointment）,and control group（diseased endothelial cell + Longzhu Ointment）.The expression of tumor necrosis factor-α（TNF--α）,interleukin-6（IL6） and endothelial nitric oxide synthase（eNOS） were detected.In vivo experiment: the rats were divided into three groups: normal group; model group（diabetic rats + Vaseline gauze） and experimental group（diabetic rats + Huiyang Shengji Ointment gauze）.The healing and morphological changes of the sore surface were observed, and the serum of rats was taken to detect the expression of TNF-α and vascular endothelial growth factor（VEGF）.Results In vitro experiment: compared with the normal group, the expression of eNOS in the model group was increased significantly and the difference was not statistically significant（P>0.05）;compared with the model group, the expression of eNOS in the experimental group and control group was higher and the difference was not statistically significant（P>0.05）; and compared with the control group, the expression of eNOS in the experiment group was increased significantly and the difference was not statistically significant（P>0.05）.Compared with the normal group, the expression of TNF-αin the model group was increased significantly and the difference was statistically significant（P<0.05）;compared with the model group, the expression of TNF-αin the experimental group was lower and the difference was statistically significant（P<0.05）;compared with the control group, the expression of TNF-αin the experimental group was lower and the difference was statistically significant（P<0.05）.Compared with the normal group, the expression of IL-6 in the model group were significantly lower and the difference was not statistically significant（P>0.05）;compared with the model group, the expression of IL-6 in the experimental group were significantly higher and the difference was statistically significant（P>0.05）; compared with the control group, the expression of IL-6 in the experimental group were significantly higher than that in the normal group and the difference was not statistically significant（P>0.05）.In vivo experiment: on the 3 rd, compared with the normal group, the wound healing rate in the model group was significantly higher and there was significant statistical difference（P< 0.05）; and compared with the model group, the wound healing rate in the experimental group was significantly higher and there was no significant statistical difference（P> 0.05）.on the 7 th day, compared with the normal group, the wound healing rate in the model group was significantly lower and there was significant difference（P<0.05）, compared with the model group, the wound healing rate in the experimental group was significantly higher and the difference was statistically significant（P<0.05）.On the 14 th day, compared with the normal group, the wound healing rate in the experimental group was significantly lower in the model group and the difference was statistically significant（P<0.05）; compared with the model group, the wound healing rate in the experimental group was significantly higher and the difference was statistically significant（P< 0.05）.On the 3 rd day, Compared with the normal group, the expression of TNF-α in the model group was significantly lower than and the difference was statistically significant（P<0.05）;compared with the model group, the expression of TNF-α in the experimental group was significantly higher, and the difference was statistically significant（P<0.05）.On the 7 th day, Compared with the normal group, the expression of TNF-α in the model group was significantly lower than and the difference was statistically significant（P<05）;compared with the model group, the expression of TNF-α in the experimental group was significantly higher, and the difference was statistically significant（P<0.05）.On the 14 th day, compared with the normal group, the expression of TNF-α in the model group was significantly higher and the difference was statistically significant（P<0.05）;compared with the model group, the expression of TNF-α in the experimental group was significantly lower, and the difference was statistically significant（P<0.05）.On the 3 rd day and 7 th day, compared with the normal group, the expression of VEGF in the model group was significantly lower and the difference was statistically significant（P<0.05）.On the 14 th day, compared with the normal group at the same time, the expression of VEGF in the experimental group was significantly lower and the difference was statistically significant（P<0.05）.On the 3 rd, 7 th, and 14 th day, compared with the model group, the difference was statistically significant（P<0.05）.Conclusion Huiyang Shengji Ointment can maintain endothelial cell homeostasis by increasing the expression of eNOS and VEGF and reducing the expression of pro-inflammatory factors, thus reducing inflammatory reaction and promoting vascular regeneration and wound repair with better effect than Longzhu Ointment.