Objective To evaluate the effect of Xihuang Pill on the proliferation and dry spheroidization ability of triple negative breast cancer cell 4 T1 based on Wnt/β-catenin signaling pathway.Methods Prepare Xihuang Pill extract, use CCK-8,select the best concentration of drug, and the effect on the proliferation ability of 4 T1 cells was tested and the effect on the dry spheroidization ability of 4 T1 cells was detected by serum-free stem cell spheroidization experiment.The effect of sphere formation ability was tested by Transwell cell migration and invasion experiment to detect the effect of Xihuangwan extract on migration and invasion ability of 4 T1 cells, and the expression of related mRNA of Wnt/cetenin pathway was detected by RT-PCR.Results The proliferation, stem spheroidizing ability, migration, invasion of tumor cell.RT-PCR Results showed that E-cadherin was up-regulated and Vimentin and β-catenin were down-regulated.Xihuang Pill could cooperate with Wnt/β-catenin pathway inhibitor XAV939 to inhibit cell proliferation, stem cell spheroidizing ability, and migration and invasion of tumor cell.Conclusion Xihuang Pill may interfere with the “dryness” of tumor cells by inhibiting the EMT transformation mediated by Wnt/β-catenin signaling pathway, thus preventing and treating tumor metastasis.