Objective To investigate the effect of Panax notoginseng saponins on cGAS/STING/NF-κB pathway and anti-inflammatory action in rats with acute cerebral infarction.Methods 30 rats were randomly divided into three groups: sham operation group, MCAO model group, and Panax notoginseng saponins treatment group（PNS,0.15 g/kg）.There were 10 rats in each group.Except for the sham operation group, The middle cerebral artery ischemia（MCAO）model were established by using thread occlusion method in other three groups, in which Panax notoginseng saponins treatment group were given Panax notoginseng saponins after established MCAO model.After 1.5 h of ischemia and 24 h of reperfusion, the Garcia JH score was carried out to observe the degree of neurological deficit.The rats were sacrificed, and the peripheral cortex was collected.The protein expression of cGAS,STING,NF-κB phosphorylation and NF-κB were detected by Western Blot.The gene expression of iNOS was measured by RT-PCR,and the levels of TNF-α and IL-6 were detected by ELISA.Results Compared with the sham operation group, the Garcia JH neurological function scores were obviously reduced in the MCAO model group（P<0.01）; the protein expression levels of cGAS,STING,and p-NF-κB/NF-κB and the levels of TNF-α and IL-6 and the gene expression level of iNOS were all significantly increased（P<0.01）.Compared with the model group, the Garcia JH neurological function scores was increased in the Panax notoginseng saponins treatment group（P<0.05）; the protein expression levels of cGAS,STING,p-NF-κB/NF-κB and the levels of TNF-α and IL-6 and the gene expression level of iNOS were all reduced（P<0.05）.Conclusion Panax notoginseng saponins may inhibit NF-κB-induced inflammatory response by regulating the cGAS/STING signaling pathway and alleviate brain damage to play a protective effect.