Study on the mechanism of Jinteng Qingbi Granules in treating rheumatoid arthritis in model rats

Jin-yang TANG; Cai-yun ZHOU; Xin WANG; Fang MA; Zheng PAN; Shu-hua HAN; Bin LI; Li-yan DU; Guo-liang CHENG; Ding-ya FANG

doi:10.16025/j.1674-1307.2022.07.006

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Study on the mechanism of Jinteng Qingbi Granules in treating rheumatoid arthritis in model rats

更新时间：2023-02-15

- Study on the mechanism of Jinteng Qingbi Granules in treating rheumatoid arthritis in model rats
- Beijing Journal of Traditional Chinese Medicine Vol. 41, Issue 7, Pages: 728-732(2022)
- 作者机构：
  
  1.中国中医科学院西苑医院风湿病科，北京 100091
  2.中药制药共性技术国家重点实验室，临沂 276000
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2022.07.006
  CLC：
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唐今扬，周彩云，王鑫，等.金藤清痹颗粒治疗类风湿关节炎模型大鼠的作用机制研究［J］.北京中医药，2022，41（7）：728-732.

TANG Jin-yang,ZHOU Cai-yun,WANG Xin,et al.Study on the mechanism of Jinteng Qingbi Granules in treating rheumatoid arthritis in model rats[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(07):728-732.
唐今扬，周彩云，王鑫，等.金藤清痹颗粒治疗类风湿关节炎模型大鼠的作用机制研究［J］.北京中医药，2022，41（7）：728-732. DOI： 10.16025/j.1674-1307.2022.07.006.

TANG Jin-yang,ZHOU Cai-yun,WANG Xin,et al.Study on the mechanism of Jinteng Qingbi Granules in treating rheumatoid arthritis in model rats[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(07):728-732. DOI： 10.16025/j.1674-1307.2022.07.006.

摘要

目的,2,探讨金藤清痹颗粒治疗类风湿关节炎（rheumatoid arthritis，RA）模型大鼠的机制。,方法,2,随机选取10只大鼠为空白对照（A）组，另取60只通过胶原诱导性关节炎法建立大鼠RA模型，造模14 d后，将关节炎指数（AI）评分≥4分的模型大鼠随机分为模型对照（B）组，金藤清痹颗粒低（C）、中（D）、高剂量（E）组，甲氨蝶呤（F）组，雷公藤多苷（G）组共6组，每组10只。C、D、E组每次分别给予1.05、2.1、4.2 g/kg金藤清痹颗粒灌胃，3次/d；F组每次给予甲氨蝶呤0.8 mg/kg灌胃，每4 d灌胃1次；G组每次给予2.625 mg/kg雷公藤多苷灌胃，3次/d灌胃。A、B组灌服等体积的0.9%氯化钠注射液。均持续灌胃4周。对比各组关节指数（AI）评分；ELISA检测外周血抗炎因子白介素（IL）⁃10、转化生长因子β,1,（TGF⁃β,1,）及促炎因子（IL⁃1β）、肿瘤坏死因子⁃α（TNF⁃α）、IL⁃17；免疫组化法检测骨保护素（OPG）/核因子κB受体活化因子配体（RANKL）、CD34表达；微血管密度（MVD）。,结果,2,与A组比较，B组大鼠外周血中IL⁃10、TGF⁃β,1,水平降低，IL⁃1β、TNF⁃α、IL⁃17水平升高（,P,<,0.05）；膝关节滑膜中OPG表达、RANKL表达、MVD值均升高（,P,<,0.05）。C、D、E、F、G组关节肿胀均较B组轻（,P,<,0.05）；D、E、F、G组外周血中IL⁃10、TGF⁃β1、TNF⁃α水平升高，IL⁃1β、IL⁃17水平降低（,P,<,0.05）；D、E、F、G组大鼠膝关节滑膜中RANKL表达、MVD值降低（,P,<,0.05），C、D、E、F、G组大鼠膝关节滑膜中OPG表达升高（,P,<,0.05）。,结论,2,金藤清痹颗粒能够抑制RA大鼠炎症反应、血管生成和OPG/RANKL表达。

Abstract

Objective,2,To study the mechanism of action of Jinteng Qingbi Granules in the treatment of rheumatoid arthritis in model rats.,Methods,2,10 rats were randomly selected as the blank control group（A），and another 60 rats were used to establish RA model by collagen-induced arthritis.Fourteen days after modeling，the model rats with arthritis index（AI）score≥4 were randomly divided into model control group（B），low（C），middle（D）and high dose（E）groups of Jinteng Qingbi Granules，methotrexate group（F）and tripterygium wilfordii polyglycoside group（G），with 6 rats in each group.Group C，D and E were respectively given 1.05，2.1 and 4.2 g/kg Jinteng Qingbi Granules by intragastric administration，three times a day.Group F was given methotrexate 0.8 mg/kg by gavage every 4 days； group g was given 2.625 mg/kg tripterygium wilfordii polyglycoside by gavage three times a day.Group A and group B were given equal volume of 0.9% sodium chloride injection.All groups were given intragastric administration for 4 weeks.The joint index （AI） scores of each group were compared. The anti-inflammatory factor interleukin （IL）⁃10， transforming growth factor β1（TGF⁃β,1,），IL⁃1β，tumor necrosis factor⁃α（TNF⁃α）and IL⁃17 in peripheral blood were detected by ELISA.Immunohistochemistry was used to detect the expression of osteoprotegerin（OPG）/nuclear factor κB receptor activator ligand（RANKL）and CD34，and Microvessel density（MVD）count.,Results,2,Compared with group A，the levels of IL⁃10 and TGF⁃β,1, in peripheral blood of rats in group B were decreased，but the levels of IL⁃1β， TNF⁃α and IL⁃17 increased（,P,<,0.05）.The expressions of OPG，RANKL and MVD in synovium of knee joint were increased（,P,<,0.05）.The joint swelling in group C，D，E，F and G was lighter than that in group B（,P,<,0.05）.In group C，D，E，F and G，the levels of IL⁃10，TGF⁃β,1, and TNF⁃α in peripheral blood were increased，while the levels of IL⁃1β and IL⁃17 decreased（,P,<,0.05）.The expressions of RANKL and MVD in synovium of knee joint of rats in groups D，E，F and G were decreased（,P,<,0.05），while the expression of OPG in synovium of knee joint of rats in groups C，D，E，F and G increased（,P,<,0.05）.,Conclusion,2,Jinteng Qingbi Granules can affect the development of RA by inhibiting inflammatory response， angiogenesis， and OPG/RANKL expression ratio.

关键词

类风湿关节炎炎症因子血管生成骨保护素/核因子κB受体活化因子配体大鼠

Keywords

rheumatoid arthritisinflammatory factorsangiogenesisopg/ranklrats

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