Identification of key genes in the progression of hepatocellular carcinoma and Chinese herbal medicines with weighted gene co-expression network analysis

LI Gen; JIA Wen-yu; WANG Ya-xin; WANG Hong-bing

doi:10.16025/j.1674-1307.2022.10.011

您当前的位置：

首页 >

文章列表页 >

Identification of key genes in the progression of hepatocellular carcinoma and Chinese herbal medicines with weighted gene co-expression network analysis

更新时间：2023-06-16

- Identification of key genes in the progression of hepatocellular carcinoma and Chinese herbal medicines with weighted gene co-expression network analysis
- Beijing Journal of Traditional Chinese Medicine Vol. 41, Issue 10, Pages: 1127-1134(2022)
- 作者机构：
  
  1.北京中医药大学临床医学院，北京 100029
  2.首都医科大学附属北京中医医院消化中心，北京 100010
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2022.10.011
  CLC：
扫描看全文
李根，贾文玉，王雅欣，等.基于加权基因共表达网络分析方法识别肝细胞癌发生发展的关键基因及治疗中药［J］.北京中医药，2022，41（10）：1127-1134.

LI Gen,JIA Wen-yu,WANG Ya-xin,et al.Identification of key genes in the progression of hepatocellular carcinoma and Chinese herbal medicines with weighted gene co-expression network analysis[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(10):1127-1134.
李根，贾文玉，王雅欣，等.基于加权基因共表达网络分析方法识别肝细胞癌发生发展的关键基因及治疗中药［J］.北京中医药，2022，41（10）：1127-1134. DOI： 10.16025/j.1674-1307.2022.10.011.

LI Gen,JIA Wen-yu,WANG Ya-xin,et al.Identification of key genes in the progression of hepatocellular carcinoma and Chinese herbal medicines with weighted gene co-expression network analysis[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(10):1127-1134. DOI： 10.16025/j.1674-1307.2022.10.011.

摘要

目的,2,应用加权基因共表达网络分析方法（WGCNA）识别肝细胞癌（hepatocellular carcinoma，HCC）发生发展的关键基因及治疗中药。,方法,2,根据癌症基因组图谱（TCGA）的数据，运用R软件包limma进行差异分析，确定肝细胞癌和正常样本之间差异表达的基因，并对差异基因做富集分析，运用加权基因共表达网络分析确定与HCC患者临床特征相关的基因模块，将基因模块中的基因与DisGeNET数据库中HCC相关的差异基因做韦恩图确定关键基因，并绘制KM曲线对关键基因进行预后评估；通过ENCORI检索相关基因的miRNA，通过Coremine Medical分析治疗关键基因的中药，并构建基因-中药网络图。,结果,2,确定了3 832个差异表达基因（1 698个表达上调，2 134个表达下调）。富集分析表明，表达上调基因主要集中在细胞有丝分裂、细胞周期调控等方面；表达下调基因主要富集于有机酸、小分子、脂质代谢等过程。WGCNA确定了10个与HCC患者临床特征相关的基因模块，包括与HCC发病相关的红色和蓝绿色模块。对后2个Hub模块的基因进行筛选，共获得2个Hub基因（CCNB1、EZH2），其表达水平与预后相关（,P,<,0.05），并获得郁金和姜黄2味治疗关键基因的中药。,结论,2,CCNB1、EZH2可能为HCC发生发展的关键基因；郁金和姜黄可能为治疗HCC的潜在中药。

Abstract

关键词

肝细胞癌生物信息学关键基因中药加权基因共表达网络

Keywords

references

刘宗超,李哲轩,张阳,等.2020全球癌症统计报告解读[J].肿瘤综合治疗电子杂志,2021,7(2):1-14.

原发性肝癌诊疗规范（2019年版）[J].传染病信息,2020,33(6):481-500.

PATEL K,LAMM R,ALTSHULER P,et al.Hepatocellular carcinoma-the influence of immunoanatomy and the role of immunotherapy[J].Int J Mol Sci,2020,21(18):6757.

赵文霞,陈欣菊.原发性肝癌中医药诊疗原则及方案构建[J].临床肝胆病杂志,2021,37(9):2005-2008.

XI SY,MINUK GY.Role of traditional Chinese medicine in the management of patients with hepatocellular carcinoma[J].World J Hepatol,2018,10(11):799-806.

CHEN L,YUAN L,WANG Y,et al.Co-expression network analysis identified FCER1G in association with progression and prognosis in human clear cell renal cell carcinoma[J].Int J Biol Sci,2017,13(11):1361-72.

KONG Q,MA Y,YU J,et al.Predicted molecular targets and pathways for germacrone, curdione, and furanodiene in the treatment of breast cancer using a bioinformatics approach[J].Sci Rep,2017,7(1):15543.

XIA Y,CHENG X,LI Y,et al.Hepatitis B virus deregulates the cell cycle to promote viral replication and a premalignant phenotype[J].J Virol,2018,92(19):e00722-18.

LIU S,YANG TB,NAN YL,et al.Genetic variants of cell cycle pathway genes predict disease-free survival of hepatocellular carcinoma[J].Cancer Med,2017,6(7):1512-1522.

MICHELOTTI A,DE SCORDILLI M,PALMERO L,et al. NAFLD-Related hepatocarcinoma:the malignant side of metabolic syndrome[J].Cells,2021,10(8):2034.

BUGIANESI E,LEONE N,VANNI E,et al.Expanding the natural history of nonalcoholic steatohepatitis: from cryptogenic cirrhosis to hepatocellular carcinoma[J].Gastroenterology,2002,123(1):134-140.

LEE SS,JEONG SH,BYOUN YS,et al.Clinical features and outcome of cryptogenic hepatocellular carcinoma compared to those of viral and alcoholic hepatocellular carcinoma[J].BMC Cancer,2013,13:335.

MITTAL S,EL-SERAG HB,SADA YH,et al.Hepatocellular carcinoma in the absence of cirrhosis in United States veterans is associated with nonalcoholic fatty liver disease[J].Clin Gastroenterol Hepatol,2016,14(1):124-131.

ZHUANG L,YANG Z,MENG Z.Upregulation of BUB1B, CCNB1,CDC7,CDC20,and MCM3 in tumor tissues predicted worse overall survival and disease-free survival in hepatocellular carcinoma patients[J].Biomed Res Int,2018,2018:7897346.

MIYAZAKI T,ARAI S.Two distinct controls of mitotic cdk1/cyclin B1 activity requisite for cell growth prior to cell division[J].Cell Cycle,2007,6(12):1419-1425.

PINES J,HUNTER T.Isolation of a human cyclin cDNA:evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2[J].Cell,1989,58(5):833-846.

LIU D,XU W,DING X,et al.Polymorphisms of CCNB1 associated with the clinical outcomes of platinum-based chemotherapy in Chinese NSCLC patients[J].J Cancer,2017,8(18):3785-3794.

RIEGER I,TSINTARI V,OVERKAMP M,et al.ASPP2κ is expressed in human colorectal carcinoma and promotes chemotherapy resistance and tumorigenesis[J].Front Mol Biosci,2021,8:727203.

ZHANG Y,QIAN J,GU C,et al.Alternative splicing and cancer:a systematic review[J].Signal Transduct Target Ther,2021,6(1):78.

SIMON JA,LANGE CA.Roles of the EZH2 histone methyltransferase in cancer epigenetics[J].Mutat Res,2008,647(1-2):21-29.

SUDO T,UTSUNOMIYA T,MIMORI K,et al. Clinicopathological significance of EZH2 mRNA expression in patients with hepatocellular carcinoma[J].Br J Cancer,2005,92(9):1754-1758.

丰平,徐大鹏,刘树红,等.调肝颗粒剂防治大鼠肝癌前病变的病理研究[J].北京中医药,2010,29(8):624-626.

章丽娜.Hedgehog信号通路在肝纤维化小鼠模型中的作用及药物干预探索[D].北京：北京协和医学院,2013.

许轶琛,朱世杰.李佩文治疗肝癌经验简介[J].北京中医药,2008,27(6):427-428.

张秋云,车念聪,高连印.钱英辨治肝癌术后临床经验[J].北京中医药,2010,29(12):905-906.

姜晓晨,张传龙,强睿,等.全国名中医朴炳奎诊治原发性肝癌临床经验拾萃[J].北京中医药,2021,40(9):926-929.

王继萱,艾宗雄,谢晶日.土鳖虫、姜黄、鳖甲、白芍治疗肝纤维化经验[J].环球中医药,2021,14(2):306-308.

樊威.重楼皂苷及其复方抗肝癌和安全性评价研究[D].天津：天津科技大学,2015.

袁晓旭,杨明明,赵桂琴.郁金化学成分及药理作用研究进展[J].承德医学院学报,2016,33(6):487-489.

魏雨菲,于海川,刘雪玲,等.姜黄主要化学成分及药理作用研究进展[J].新乡医学院学报,2020,37(10):990-995.

任浩.SWRG活性成分体内过程研究[D].南京:南京中医药大学,2014.

MEHTA HJ,PATEL V,SADIKOT RT.Curcumin and lung cancer：a review[J].Target Oncol,2014,9(4):295-310.

蒋烈浩,殷珂欣,葛明华.姜黄素抗肿瘤作用及相关机制的研究进展[J].江苏医药,2018,44(7):830-834.

刘伟,顾秀竹,吴筱霓,等.姜黄素药理作用的研究进展[J].华西药学杂志,2021,36(3):336-340.

陈美霓,郭巍,郝琴,等.姜黄素的药理作用、临床应用及机制研究进展[J].延安大学学报(医学科学版),2021,19(3):96-99.

姚平,阮萍,林国彪.microRNA在肝细胞肝癌中的诊断及治疗研究进展[J].中国继续医学教育,2021,13(32):195-198.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on drug properties and syndrome-symptom-formula-herb networks of traditional Chinese medicine in treatment of common chronic diseases with method of brain-gut coordinating

Research progress on the effect of Chinese herbal medicine on PI3K/Akt signal pathway in diabetic nephropathy

Clinical observation on treatment of cervical spondylotic radiculopathy of qi stagnation and blood stasis type with combination of traditional Chinese and western medicine

A case of alopecia universalis in children treated with oral taking of Chinese herbal medicine

Related Author

No data

Related Institution

天津中医药大学中医学院

中国医学科学院北京协和医学院北京协和医院中医科

北京市东城区第一人民医院针灸科

北京市东城区第一人民医院康复科

北京中医药大学东直门医院针灸科

⁰