Effect and mechanism of Qingre Jianpi Tiaochang Recipe in DSS-induced colitis model mice

ZHANG Tao; GONG Zhao-yuan; WEI Wei; SU Xiao-lan

doi:10.16025/j.1674-1307.2022.10.017

您当前的位置：

首页 >

文章列表页 >

Effect and mechanism of Qingre Jianpi Tiaochang Recipe in DSS-induced colitis model mice

更新时间：2023-06-16

- Effect and mechanism of Qingre Jianpi Tiaochang Recipe in DSS-induced colitis model mice
- Beijing Journal of Traditional Chinese Medicine Vol. 41, Issue 10, Pages: 1158-1163(2022)
- 作者机构：
  
  1.中国中医科学院望京医院脾胃病科功能性胃肠病中医诊治北京市重点实验室，北京 100102
  2.中国中医科学院中医临床基础医学研究所，北京 100700
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2022.10.017
  CLC：
扫描看全文
张涛，龚照元，魏玮，等.清热健脾调肠方对DSS结肠炎小鼠的作用及机制［J］.北京中医药，2022，41（10）：1158-1163.

ZHANG Tao,GONG Zhao-yuan,WEI Wei,et al.Effect and mechanism of Qingre Jianpi Tiaochang Recipe in DSS-induced colitis model mice[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(10):1158-1163.
张涛，龚照元，魏玮，等.清热健脾调肠方对DSS结肠炎小鼠的作用及机制［J］.北京中医药，2022，41（10）：1158-1163. DOI： 10.16025/j.1674-1307.2022.10.017.

ZHANG Tao,GONG Zhao-yuan,WEI Wei,et al.Effect and mechanism of Qingre Jianpi Tiaochang Recipe in DSS-induced colitis model mice[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(10):1158-1163. DOI： 10.16025/j.1674-1307.2022.10.017.

摘要

目的,2,观察清热健脾调肠方对葡聚糖硫酸钠（DSS）结肠炎小鼠的作用及对脾脏T淋巴细胞表达的影响，探讨该方治疗DSS结肠炎的作用机制。,方法,2,选用36只SPF级C57BL/6小鼠，分为正常组，模型组，对照组，中药高、中、低剂量组，每组各6只。除正常组外，其余小鼠采用2.5% DSS水溶液诱导小鼠DSS结肠炎模型。造模结束后，中药高、中、低剂量组分别以清热健脾调肠方0.88、0.44、0.22 g/（kg·d）灌胃；对照组予美沙拉嗪缓释颗粒混悬液0.52 g/（kg·d）灌胃；正常组、模型组分别予等体积生理盐水灌胃。各组均干预7 d。观察各组小鼠进食量、体质量、疾病活动指数（DAI）、结肠长度和组织病理学；ELISA检测血清细胞因子TNF-α、IL-10和C反应蛋白（CRP）水平；流式细胞术检测小鼠脾脏中Treg、Th17、CD4,+,、CD8,+,细胞比例。,结果,2,中药中、高剂量组可增加结肠炎小鼠摄食量、体质量，恢复结肠形态，改善病理损伤，降低DAI，小鼠脾脏Treg、CD4,+,细胞比例升高，Th17、CD8,+,细胞比例降低，与模型组比较，差异均有统计学意义（,P,<,0.05）；与模型组比较，中药中、高剂量组和对照组小鼠血清TNF-α、CRP水平降低（,P,<,0.05），IL-10水平升高（,P,<,0.05）。,结论,2,清热健脾调肠方能够增加DSS结肠炎小鼠体质量、进食量，降低DAI评分，减轻肠黏膜损伤，其机制可能是通过调节DSS结肠炎小鼠脾脏T淋巴细胞及相关细胞因子的表达及平衡，从而发挥免疫调节作用。

Abstract

Objective,2,To observe the effect of Qingre Jianpi Tiaochang Recipe in mice with DSS-induced colitis and the expression of spleen T lymphocytes， and to explore the mechanism of the recipe in treating DSS-induced colitis.,Methods,2,36 SPF C57BL/6 mice were selected and divided into normal group， model group， control group， high， medium， and low doses of Chinese medicine treatment group with 6 in each group. Except the normal group， the other groups of mice were given 2.5% DSS solution to induce ulcerative colitis model. After the molding is finished， the three Chinese medicine treatment groups were intragastrically administered with high， medium， and low doses of Qingre Jianpi Tiaochang Recipe， the control group was intragastrically administered with Salazine sustained-release granule suspension 0.52 g/（kg·d）， the normal and model groups were intragastrically administered with an equal volume of physiological saline. All groups got intervention for 7 days. Food intake， body weight， DAI， colon length and histopathology of mice in each group were observed. Serum levels of cytokines TNF-α， IL-10 and C-reactive protein （CRP） were determined by Elisa.The expression of Treg， Th17， CD4,+, and CD8,+, cells in mouse spleen was detected by flow cytometry.,Results,2,Food intake and body weight were increased， colonic morphology restored， the pathological injury improved and DAI reduced， the proportion of Treg and CD4,+, cells in spleen of mice increased， while the proportion of Th17 and CD8,+, cells decreased in the medium and high doses of Chinese medicine groups. Compared with model group， the difference was not statistically significant （,P,>,0.05）； compared with the model group， the serum TNF-α and CRP contents were lower （,P,<,0.05） and the IL-10 content was higher （,P,<,0.05） in the medium and high dose groups of Chinese medicine.,Conclusion,2,Qingre Jianpi Tiaochang Recipe can restore the body weight， increase food intake， decrease DAI score and reduce intestinal mucosal injury in UC mice.The mechanism may be mediated by regulating the expression and balance of spleen T lymphocytes and related cytokines in UC mice， so as to play an immunomodulatory role.

关键词

DSS结肠炎溃疡性结肠炎清热健脾调肠方T淋巴细胞动物实验小鼠

Keywords

DSS colitisulcerative colitisQingre Jianpi Tiaochang RecipeT lymphocytesanimal experimentsmice

references

NG SC, SHI HY, HAMIDI N, et al. Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: a systematic review of population-based studies[J]. Lancet, 2017,390(10114):2769-2778.

DANESE S, ALLEZ M, VAN BODEGRAVEN AA, et al. Unmet medical needs in ulcerative colitis: an expert group consensus[J]. Dig Dis,2019,37(4):266-283.

WIRTZ S, POPP V, KINDERMANN M, et al. Chemically induced mouse models of acute and chronic intestinal inflammation[J]. Nat Protoc.2017;12(7):1295-1309.

徐叔云，卞如濂，陈修. 药理实验方法学［M］. 北京:人民卫生出版社，2002:202-203．

LADIELEMAN, PALMEN MJ, AKOL H, et al. Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines[J]. Clin Exp Immunol,1998,114(3):385-391.

HIRATA Y, IHARA S, KOIKE K. Targeting the complex interactions between microbiota, host epithelial and immune cells in inflammatory bowel disease[J]. Pharmacol Res,2016,113(Pt A):574-584.

刘端勇, 徐荣, 黄敏芳,等. 香参丸对结肠炎大鼠小肠派伊尔结中T细胞亚群及IL-2、TGF-β表达的调节[J]. 中成药,2015,37(11):2524-2526.

宋亚芳, 裴丽霞, 赵婷婷. 溃疡性结肠炎免疫因素发病机制的研究进展[J]. 医学研究生学报,2019,32(4):432-436.

李佩容, 危蓉, 吴志平,等. Th17/Treg细胞及其相关细胞因子在溃疡性结肠炎发病中的作用及其研究进展[J]. 世界科技研究与发展,2017,39(2):194-197.

EASTAFF-LEUNG N, MABARRACK N, BARBOUR A, et al. Foxp3+ regulatory T cells, Th17 effector cells, and cytokine environment in inflammatory bowel disease[J]. J Clin Immunol.2010;30(1):80-89.

马海芹, 赵小静, 张红杰. 血清炎性活动指标、Th17/Treg细胞在溃疡性结肠炎患者中的改变及其临床意义[J]. 胃肠病学和肝病学杂志,2018,27(8):885-890.

何智超, 林楠, 尹丕发. Th17/Treg细胞失衡与溃疡性结肠炎患者疾病活动度及炎症程度的关系[J]. 中国医药导报,2019,16(5):130-133.

刘震宇, 曹晖. 溃疡性结肠炎外周血中Th17细胞、Treg细胞及Th17/Treg细胞表达Meta分析[J]. 实用中医内科杂志,2020,34(6):109-113.

谢正兴, 邸雅南, 彭德银,等. HIF-1α、CRP在溃疡性结肠炎患者血清中表达的意义[J]. 现代医学,2017,45(10):1468-1471.

宋爱玲. TNF-α、IL-6、IL-8与溃疡性结肠炎严重程度相关研究[J]. 中国实用医药,2008,3(36):3-4.

张晓斐, 蒋文瑜, 于鹏丽,等. Th17细胞及其相关因子在活动期溃疡性结肠炎患者中的表达及意义[J]. 世界华人消化杂志,2013,21(1):19-26.

MAN AL, GICHEVA N, NICOLETTI C. The impact of ageing on the intestinal epithelial barrier and immune system[J]. Cell Immunol,2014,289(1-2):112-118.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Therapeutic effect and mechanism of Weichang’an Pill on dextran sodium sulfate-induced ulcerative colitis in mice

Study on the regulatory effect of Yuxian Formula on retention enema on PXR/NF-κB signaling pathway and inflammatory factors in rats with ulcerative colitis of damp-heat type in large intestine

Effects of different ranges of cold ablation on Lewis lung cancer mouse Tregs cells

Mechanism of Tangluoning for alleviating pyroapoptosis of sciatic nerve cells in diabetic mice by SIRT1

Mechanism of Rhizoma coptidis in improving diarrhea in ulcerative colitis through regulating the expression of AQPs protein in colonic mucosal epithelial cells

Related Author

No data

Related Institution

Beijing University of Chinese Medicine

Department of Spleen and Stomach Diseases of Wangjing Hospital,China Academy of Chinese Medical Sciences,Key Laboratory of TCM Diagnosis and Treatment of Functional Gastrointestinal Diseases

Spleen and Stomach Diseases Department， Yangzhou Hospital Affiliated to Nanjing University of Traditional Chinese Medicine

Department of Oncology，Dongfang Hospital，Beijing University of Chinese Medicine

Clinical Trial Center，Dongfang Hospital，Beijing University of Chinese Medicine

⁰