Effect of Tangbikang on the expression of p-JNK and Bcl-2 in sciatic nerve of diabetes rats

ZHANG Yan; ZHAO Dan; WANG Xi-peng; LI Yang-fan; QIN Fang-fang; HAN Chao-ru; LIU Ke-jia; HOU Dan; LIU Tong-hua; WU Li-li

doi:10.16025/j.1674-1307.2022.11.003

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Effect of Tangbikang on the expression of p-JNK and Bcl-2 in sciatic nerve of diabetes rats

更新时间：2023-02-15

- Effect of Tangbikang on the expression of p-JNK and Bcl-2 in sciatic nerve of diabetes rats
- Beijing Journal of Traditional Chinese Medicine Vol. 41, Issue 11, Pages: 1216-1221(2022)
- 作者机构：
  
  1.首都医科大学附属北京友谊医院中医科，北京 100050
  2.渭南市第二医院呼吸与危重症医学科，陕西渭南 714099
  3.北京第二外国语学院校医院，北京 100011
  4.北京中医药大学第三附属医院呼吸科，北京 100029
  5.北京中医药大学中医学院，北京 100029
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2022.11.003
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张岩，赵丹，王曦鹏，等.糖痹康对糖尿病大鼠坐骨神经p-JNK和Bcl-2表达的影响［J］.北京中医药，2022，41（11）：1216-1221.

ZHANG Yan,ZHAO Dan,WANG Xi-peng,et al.Effect of Tangbikang on the expression of p-JNK and Bcl-2 in sciatic nerve of diabetes rats[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(11):1216-1221.
张岩，赵丹，王曦鹏，等.糖痹康对糖尿病大鼠坐骨神经p-JNK和Bcl-2表达的影响［J］.北京中医药，2022，41（11）：1216-1221. DOI： 10.16025/j.1674-1307.2022.11.003.

ZHANG Yan,ZHAO Dan,WANG Xi-peng,et al.Effect of Tangbikang on the expression of p-JNK and Bcl-2 in sciatic nerve of diabetes rats[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(11):1216-1221. DOI： 10.16025/j.1674-1307.2022.11.003.

摘要

目的,2,观察糖痹康对糖尿病大鼠坐骨神经磷酸化c-Jun氨基末端激酶（p-JNK）和B淋巴细胞瘤-2基因（Bcl-2）表达的影响。,方法,2,雄性SD大鼠70只，选取10只为正常组，其余60只予高脂饲料与小剂量链脲佐菌素（STZ）联合诱发糖尿病大鼠模型，将造模成功的糖尿病大鼠分为4组：模型组、α-硫辛酸（ALA）组［0.0268 g/（kg·d）］、糖痹康高剂量组［2.5 g/（kg·d）］、糖痹康低剂量组［1.25 g/（kg·d）］。治疗16周后行坐骨神经HE染色，Western blot检测大鼠坐骨神经中p-JNK和Bcl-2蛋白的表达；RT-PCR检测大鼠坐骨神经中Bcl-2 mRNA的表达。,结果,2,干预16周后，与模型组相比，光镜下显示ALA组、糖痹康高剂量组、糖痹康低剂量组坐骨神经纤维结构较规整，神经纤维的变性和断裂较少，形态改变较小；ALA组、糖痹康高剂量组、糖痹康低剂量组p-JNK蛋白表达明显低于模型组（,P,<,0.05，,P,<,0.01）；糖痹康高剂量组大鼠坐骨神经Bcl-2蛋白表达与模型组比较升高（,P,<,0.05），ALA组、糖痹康低剂量组大鼠坐骨神经Bcl-2蛋白表达与模型组比较有升高趋势，但差异无统计学意义（,P,>,0.05）；ALA组、糖痹康高剂量组、糖痹康低剂量组Bcl-2 mRAN表达明显高于模型组（,P,<,0.01）。,结论,2,中药复方糖痹康能够下调糖尿病大鼠坐骨神p-JNK蛋白表达，上调Bcl-2蛋白和mRAN表达，可能是糖痹康保护糖尿病周围神经病变大鼠坐骨神经的作用靶点之一。

Abstract

Objective,2,To observe the effect of Tangbikang on the expression of phosphorylated c-Jun N-terminal kinase （p-JNK） and B-cell lymphama-2 （Bcl-2） in sciatic nerve of diabetes rats.,Methods,2,10 male SD rats were randomly selected from 70 male SD rats as the normal group， and the other 60 rats were given high-fat diet and low-dose streptozotocin （STZ） to induce type 2 diabetic rat model. After modeling，diabetic rats were randomly divided into 4 groups： model group， α- Alpha lipoic acid （ALA） group［0.0268g/（kg·d）］， Tangbikang high dose group［2.5 g/（kg·d）］， Tangbikang low dose group ［1.25 g/（kg·d）］. After 16 weeks， morphological changes were observed by HE staining. The proteins expression of p-JNK and Bcl-2 were detected by Western blot. The expression of Bcl-2 mRNA was detected by real-time PCR.,Results,2,After 16 weeks of treatment， compared with the model group， the structure of sciatic nerve fibers in ALA group，Tangbikang high group and Tangbikang low dose group were more regular， and the degeneration and breakage of nerve fibers were less， and their morphology were not much changed. The expression of p-JNK protein in ALA group， Tangbikang high and low dose groups were significantly lower than that in model group （,P,<,0.05，,P,<,0.01）. The expression of Bcl-2 protein in sciatic nerve of rats in Tangbikang high dose group was higher than that in model group （,P,<,0.05）. The expression of Bcl-2 protein in sciatic nerve of rats in ALA group and low dose group were higher than that in model group， but there were no statistical significance （,P,>,0.05）. The expression of Bcl-2 mRNA in ALA group， Tangbikang high and low dose groups were significantly higher than that in model group （,P,<,0.01）.,Conclusion,2,Tangbikang can down regulate the expression of p-JNK protein and up regulate the expression of Bcl-2 protein and mRNA in sciatic nerve of DPN rats，which may be one of the targets of Tangbikang in protecting sciatic nerve of DPN rats.

关键词

糖痹康糖尿病周围神经病变坐骨神经磷酸化c-Jun氨基末端激酶B淋巴细胞瘤-2基因大鼠

Keywords

Tangbikangdiabetic peripheral neuropathysciatic nervephosphorylated c-Jun N-terminal kinaseB-cell lymphoma-2

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首都医科大学附属北京友谊医院医务处

首都医科大学中医药学院

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Department of Endocrinology， Dongfang Hospital， Beijing University of Chinese Medicine

中国中医科学院广安门医院心血管科

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