Mechanism of Tangluoning for alleviating pyroapoptosis of sciatic nerve cells in diabetic mice by SIRT1

WU Chang-jiang; WANG Xiao-lei; ZHANG Chen; XIN Jing-yan; XIE Wen-ying; LI Wen-hui; ZHUO Zhen-nan; HU Hao; ZHANG Tao-jing

doi:10.16025/j.1674-1307.2022.11.004

您当前的位置：

首页 >

文章列表页 >

Mechanism of Tangluoning for alleviating pyroapoptosis of sciatic nerve cells in diabetic mice by SIRT1

更新时间：2023-02-15

- Mechanism of Tangluoning for alleviating pyroapoptosis of sciatic nerve cells in diabetic mice by SIRT1
- Beijing Journal of Traditional Chinese Medicine Vol. 41, Issue 11, Pages: 1222-1225(2022)
- 作者机构：
  
  1.北京中医药大学第二临床医学院，北京 100029
  2.北京中医药大学东方医院内分泌科，北京 100078
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2022.11.004
  CLC：
扫描看全文
吴长江，王晓磊，张晨，等.糖络宁通过SIRT1减轻糖尿病小鼠坐骨神经细胞焦亡的机制研究［J］.北京中医药，2022，41（11）：1222-1225.

WU Chang-jiang,WANG Xiao-lei,ZHANG Chen,et al.Mechanism of Tangluoning for alleviating pyroapoptosis of sciatic nerve cells in diabetic mice by SIRT1[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(11):1222-1225.
吴长江，王晓磊，张晨，等.糖络宁通过SIRT1减轻糖尿病小鼠坐骨神经细胞焦亡的机制研究［J］.北京中医药，2022，41（11）：1222-1225. DOI： 10.16025/j.1674-1307.2022.11.004.

WU Chang-jiang,WANG Xiao-lei,ZHANG Chen,et al.Mechanism of Tangluoning for alleviating pyroapoptosis of sciatic nerve cells in diabetic mice by SIRT1[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(11):1222-1225. DOI： 10.16025/j.1674-1307.2022.11.004.

摘要

目的,2,探讨糖络宁通过沉默信息调节因子2相关酶1（SIRT1）减轻KK-Ay糖尿病小鼠坐骨神经细胞焦亡的机制。,方法,2,选用8周龄SPF级雄性C57BL/6J小鼠10只，普通饲料喂养作为空白对照组；8周龄SPF级雄性KK-Ay小鼠35只，高脂饲料喂养4周，检测小鼠随机血糖≥16.7 mmol/L视为糖尿病小鼠模型造模成功。将造模成功的30只小鼠随机分为模型组、糖络宁低剂量组、糖络宁高剂量组3个组。糖络宁低剂量组按生药10 g/（kg·d）灌胃，糖络宁高剂量组按生药20 g/（kg·d）灌胃，空白对照组和模型组予等体积蒸馏水灌胃，实验期间自由饮食，连续给药12周。采用透射电镜观察坐骨神经细胞形态结构，Western blot检测SIRT1及焦亡相关分子Caspase1、NOD样受体热蛋白结构域相关蛋白3（NLRP3）、膜穿孔蛋白D（GSDMD）的表达水平。,结果,2,与空白对照组比较，模型组小鼠SIRT1蛋白表达水平显著下调（,P,<,0.05），Caspase1、NLRP3、GSDMD蛋白表达水平升高（,P,<,0.05）；与模型组比较，糖络宁高剂量组SIRT1蛋白表达显著升高（,P,<,0.05），Caspase1、NLRP3和GSDMD蛋白表达水平均降低（,P,<,0.05）。,结论,2,糖络宁可以减轻糖尿病周围神经病变小鼠坐骨神经细胞的焦亡，其部分作用机制可能是通过上调SIRT1实现，而高剂量的糖络宁效果更加明显。

Abstract

Objective,2,To investigate the mechanism of Tangluoning for alleviating pyrodeath of sciatic nerve cells in KK-Ay diabetic mice by SIRT1.,Methods,2,Ten SPF male C57BL/6J mice aged 8 weeks with ordinary feed were selected as blank control group and 30 SPF male KK-Ay mice aged 8 weeks were fed with high fat diet for 4 weeks. Random blood glucose ≥16.7mmol/L was detected as diabetic mouse model was successfully established.The successfully modeled mice were randomly divided into three groups：model group，low-dose Tangluoning group and high-dose Tangluoning group.The low-dose Tangluoning group was given intragastric administration of crude drug 10g/（kg•d），the high-dose Tangluoning group was given intragastric administration of crude drug 20g/（kg•d），the blank control group and the model group were given intragastric administration of equal volume distilled water， free diet during the experiment， continuous administration for 12 weeks.The morphological structure of sciatic nerve cells was observed by transmission electron microscopy，and the expression levels of SIRT1 and scorched-death related molecules （Caspase1， NLRP3， GSDMD） were detected by Western blot.,Results,2,Compared with the blank control group， the expression level of SIRT1 protein in model group was significantly down-regulated（,P,<,0.05）， the protein expression levels of Caspase1， NLRP3 and GSDMD increased （,P,<,0.05）；compared with the model group， SIRT1 protein expression in Tangluoning high-dose group was significantly increased （,P,<,0.05），Caspase1， NLRP3 and GSDMD protein expression levels were decreased （,P,<,0.05）.,Conclusion,2,Tangluoning can reduce scorch death of sciatic nerve cells in DPN mice，and part of its mechanism may be realized through up-regulation of SIRT1， and the effect of high-dose Tangluoning is more obvious.

关键词

糖络宁SIRT1坐骨神经细胞焦亡小鼠

Keywords

TangluoningSIRT1sciatic nervepyroptosismice

references

SUN H, SAEEDI P, KARURANGA S, et al. IDF diabetes atlas: Global,regional and country-level diabetes prevalence estimates for 2021 and projections for 2045[J].Diabet Res Clin Pract,2021,183(10267):109119.

HICKS CW, SELVIN E. Epidemiology of peripheral neuropathy and lower extremity disease in diabetes[J].Current Diabetes Reports,2019,19(10):86.

CALLAGHAN BC, PRICE RS, CHEN KS, et al. The importance of rare subtypes in diagnosis and treatment of peripheral neuropathy: a review[J]. JAMA Neurol,2015,72:1510-1518.

ANDERSEN ST, WITTE DR, DALSGAARD EM, et al. Risk factors for incident polyneuropathy in a cohort with screen-detected type 2 diabetes followed for 13 years: ADDITION-Denmark[J]. Diabetes Care,2018,41(5):1068-1075.

MAMUN AA, WU Y, NASRIN F,et al. Role of pyroptosis in diabetes and its therapeutic implications[J]. J Inflamm Res,2021,24(14):2187-2206.

XU J, CAI S, ZHAO J,et al. Advances in the relationship between pyroptosis and diabetic neuropathy[J]. Front Cell Dev Biol,2021,12(9):753660.

姚伟洁, 杨鑫伟, 李潇，等，糖络宁对高血糖大鼠血清外泌体中miR-322和IRE1α-RIDD表达的影响[J]. 中草药,2021,52(6):1654-1661.

JIA W, BAI T, ZENG J，et al. Combined administration of metformin and atorvastatin attenuates diabetic cardiomyopathy by inhibiting inflammation, apoptosis, and oxidative stress in type 2 diabetic mice[J]. Front Cell Dev Biol，2021,16(9):634900.

宋荷花, 魏在荣. 糖尿病的周围神经病变：研究与治疗[J]. 中国组织工程研究,2023,27(8):1278-1285.

梅旦, 张玲玲, 魏伟. 细胞焦亡机制及与疾病的关系[J]. 生理科学进展,2020,51(2):151-156.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of different ranges of cold ablation on Lewis lung cancer mouse Tregs cells

Effect of Tangbikang on the expression of p-JNK and Bcl-2 in sciatic nerve of diabetes rats

Effect and mechanism of Qingre Jianpi Tiaochang Recipe in DSS-induced colitis model mice

Effects of Tangluoning on IRE1α-CHOP pathway of dorsal root ganglion in peripheral neuropathy rats under high glucose environment

Effect mechanism of Feiliuping Ointment on inhibition of Lewis lung cancer by regulating NF-κB signaling pathway in tumor inflammatory microenvironment

Related Author

No data

Related Institution

Department of Oncology，Dongfang Hospital，Beijing University of Chinese Medicine

Beijing University of Chinese Medicine

Clinical Trial Center，Dongfang Hospital，Beijing University of Chinese Medicine

Department of Traditional Chinese Medicine， Beijing Friendship Hospital Affiliated to the Capital Medical University

Department of Respiratory and Critical Care Medicine， Weinan Second Hospital

⁰