Objective,2,To explore the effect and mechanism of Qingshi Zhiyang Ointment on inflammation model of human immortalized keratinocytes （HaCaT） and epidermal keratinocyte induced by tumor necrosis factor-α （TNF-α）/interferon-γ （IFN-γ）.,Methods,2,HaCaT cells and human epidermal keratinocytes were divided into blank group， model group and experimental group respectively. The blank group received no intervention measures. The model group was stimulated with TNF-α（10 ng/mL）+IFN-γ（10 ng/mL） for 6 h to induce inflammation model. On the basis of modeling， the experimental group was cultured with 0.000 1， 0.001， 0.01 and 0.1 g/mL of Qingshi Zhiyang ointment， and divided into four subgroups according to the above concentrations. The effect of Qingshi Zhiyang ointment on cell viability was determined by cell counting kit -8 method. Quantify microRNA-145 （miRNA-145）was detected by real-time reverse transcriptase polymerase chain reaction（real-time RT-PCR） method，Western Blot （WB） method to detect the expression of Sema3A protein，enzyme linked immunosorbent assay （ELISA） method to determine the expression level of interleukin-31 （IL-31）， NGF， nerve growth factor 4 （NT-4）.,Results,2,HaCaT cells： Qingshi Zhiyang Ointment with different concentrations has no obvious inhibition on cell proliferation. Compared with the blank group， the levels of miRNA-145 was decreased and Sema3A protein increased， and the levels of IL-31， NGF， and NT-4 increased （,P,<,0.05）. Compared with the model group， the expression of miRNA-145 in 0.001 g/mL group， 0.01 g/mL group and 0.1 g/mL group all increased （,P,<,0.05）. Sema3A protein expression decreased （,P,<,0.05）. The levels of IL-31， NGF and NT-4 in cell culture supernatants of 0.001 g/mL， 0.001 g/ml， 0.01 g/mL and 0.1 g/mL groups decreased gradually， and the differences among the latter three groups were statistically significant （,P,<,0.05）. Human epidermal keratinocytes ： compared with the blank group， the expression of miRNA-145 in the model group was lowered， the expression of Sema3A protein was increased， and the levels of IL-31， NGF and NT-4 in cell culture supernatant were higher （,P,<,0.05）. Compared with the model group， the expression of miRNA-145 in 0.01 g/mL group and 0.1 g/mL group was increased （,P,<,0.05）. The expression of Sema3A protein in 0.001 g/mL， 0.01g/mL and 0.1 g/mL groups was decreased （,P,<,0.05）. The levels of IL-31， NGF and NT-4 in cell culture supernatants of 0.01 g/mL and 0.1 g/mL groups were decreased （,P,<,0.05）， while the levels of IL-31 and NGF in cell culture supernatants of 0.001 g/mL and 0.001g/ml groups were decreased （,P,<,0.05）.,Conclusion,2,Qingshi Zhiyang Ointment can significantly improve the inflammation of HaCaT cells and human epidermal keratinocytes. The mechanism may be through regulating the level of Sema3A/NGF and related molecules to reduce inflammation.