Effects of different ranges of cold ablation on Lewis lung cancer mouse Tregs cells

ZHUANG Yao-xue; LIU Dian-na; GAO Lei; SUN jing-yi; LIN Shi-cheng; WEI Jia; WANG Xiao-fan; LI Quan-wang

doi:10.16025/j.1674-1307.2023.03.011

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Effects of different ranges of cold ablation on Lewis lung cancer mouse Tregs cells

更新时间：2023-06-16

- Effects of different ranges of cold ablation on Lewis lung cancer mouse Tregs cells
- Beijing Journal of Traditional Chinese Medicine Vol. 42, Issue 3, Pages: 275-280(2023)
- 作者机构：
  
  1.北京中医药大学，北京 100029
  2.北京中医药大学东方医院临床试验中心，北京 100078
  3.北京中医药大学东方医院肿瘤科，北京 100078
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2023.03.011
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庄垚雪，刘殿娜，高磊，等.不同范围冷消融治疗对Lewis肺癌小鼠Tregs细胞的影响［J］.北京中医药，2023，42（3）：275-280.

ZHUANG Yao-xue,LIU Dian-na,GAO Lei,et al.Effects of different ranges of cold ablation on Lewis lung cancer mouse Tregs cells[J]. Beijing Journal of Traditional Chinese Medicine,2023,42(03):275-280.
庄垚雪，刘殿娜，高磊，等.不同范围冷消融治疗对Lewis肺癌小鼠Tregs细胞的影响［J］.北京中医药，2023，42（3）：275-280. DOI： 10.16025/j.1674-1307.2023.03.011.

ZHUANG Yao-xue,LIU Dian-na,GAO Lei,et al.Effects of different ranges of cold ablation on Lewis lung cancer mouse Tregs cells[J]. Beijing Journal of Traditional Chinese Medicine,2023,42(03):275-280. DOI： 10.16025/j.1674-1307.2023.03.011.

摘要

目的,2,探究不同范围冷消融治疗对Lewis肺癌小鼠Tregs细胞的影响。,方法,2,采用Lewis小鼠肺腺癌细胞株建立C57BL/6小鼠皮下移植瘤模型，选取造模成功的小鼠随机分为对照组、冷消融低范围组、冷消融中范围组、冷消融全范围组，每组5只。冷消融组予以不同冷冻范围的冷消融手术，冷消融低范围组消融面积为肿瘤的50%~60%，中范围组为60%~90%，全范围组为100%；对照组予以假手术治疗。术后14日取外周血、脾脏及肿瘤组织。流式细胞仪检测肿瘤组织、脾脏中CD4,+,CD25,+,FoxP3,+,T细胞数量；Western blot法检测肿瘤组织中Foxp3蛋白表达情况；qRT-PCR检测肿瘤组织中Foxp3 mRNA表达；ELISA检测外周血中细胞因子白介素（IL）-2、转化生长因子（TGF）-β的表达。,结果,2,与对照组相比，冷消融中范围组脾脏组织中CD4,+,CD25,+,FoxP3,+,T细胞比例降低（,P,<,0.05），冷消融各组组间相比差异无统计学意义（,P,>,0.05）；与对照组相比，冷消融全范围组肿瘤组织中CD4,+,CD25,+,FoxP3,+,T细胞比例降低（,P,<,0.05），冷消融各组组间相比差异无统计学意义（,P,>,0.05）。与对照组相比，冷消融各组Foxp3蛋白、mRNA表达低（,P,<,0.05），中范围、全范围组低于低范围组（,P,<,0.05），中范围组与全范围组相比差异无统计学意义（,P,>,0.05）。与对照组相比，冷消融各组外周血中IL-2水平均高（,P,<,0.05），中范围组、全范围组均高于低范围组（,P,<,0.05），全范围组低于中范围组（,P,<,0.05）。与对照组相比，冷消融低范围组、中范围组、全范围组TGF⁃β水平均低（,P,<,0.05），低范围组、全范围组均高于中范围组（,P,<,0.05），全范围组高于低范围组和中范围组（,P,<,0.05）。,结论,2,冷消融治疗不仅可直接引起肿瘤组织机械性损伤，而且可能通过抑制Tregs细胞表达抑制残余肿瘤的生长；冷消融范围60%~100%的效果优于冷消融低范围50%~60%。

Abstract

Objective,2,To investigate the effects of cold ablation of different ranges on the Tregs cells of Lewis lung cancer mice.,Methods,2,The subcutaneous transplantation tumor model of C57BL/6 mice was developed using Lewis mouse lung adenocarcinoma cell lines. Five mice were randomly assigned to four groups，including control group， low-range cryoablation group， medium-range cryoablation group， and full-range cryoablation group. The cold ablation group was given cold ablation with different freezing ranges，the ablation area of the low-range group was 50%~60% of the tumor， the middle-range group was 60%~90%， and the full-range group was 100%， whereas the control group underwent sham surgery. Periperalblood， spleen， and tumor tissue were collected 14 days after surgery. The number of CD4,+,CD25,+,FoxP3,+ ,T cells in tumor tissue and spleen was determined using flow cytometry. Western blot was used to detect the expression of Foxp3 protein in tumor tissues， qRT-PCR to detect the expresssion of Foxp3 mRNA in tumor tissues. In addition， the expression of cytokines IL-2 and TGF-β in peripheral blood was detected using ELISA.,Results,2,Compared with the control group， the proportion of CD4,+,CD25,+,FoxP3,+, T cells in the spleen tissue of the middle-range of cold ablation group was decreased with difference of three cold ablation groups statistically significant（,P,<,0.05 ）. Compared with the control group，the proportion of CD4,+,CD25,+,FoxP3,+,T cells in the whole range of cold ablation group was decreased with the difference of three cold ablation groups not statistically significant（,P,>,0.05 ）. Compared with the control group，the expressions of Foxp3 protein and mRNA in cold ablation groups were lower（,P,<,0.05），and those in the middle and full range groups were lower than those in the low range group（,P,<,0. 05）. There was no statistical difference between the middle range group and the full range group （,P,>,0.05）. Compared with the control group，the level of IL-2 in peripheral blood of each cold ablation group was higher（,P,<,0.05），the middle range group and the full range group were higher than the low range group （,P,<,0.05）， and the full range group was lower than the middle range group（,P,<,0.05）. Compared with the control group， the level of TGF-β in the low range group， the middle range group and the full range group was lower（,P,<,0.05）. The low range group and the full range group were higher than the middle range group （,P,<,0.05），and the full range group was higher than the low range group and the middle range group（,P,<,0.05）.,Conclusion,2,In addition to directly causing mechanical damage to tumor tissue， cold ablation can also inhibit the growth of the residual tumor by inhibiting the expression of Tregs cells. The efficacy of cold ablation groups in the range of 60%~100% was superior to that of low range. The effect of cold ablation in the range of 60%~100% is better than that in the low range of 50%~60%.

关键词

肺癌冷消融叉头翼状螺旋转录因子白介素-2转化生长因子-β小鼠

Keywords

lung cancercold ablationFoxp3IL-2TGF-βmice

references

SIEGEL RL, MILLER KD, FUCHS HE, et al. Cancer statistics, 2022[J]. CA Cancer J Clin, 2022,72(1):7-33.

CHEN P, LIU Y, WEN Y, et al. Non-small cell lung cancer in China[J]. Cancer Commun (Lond), 2022,42(10):937-970.

SUNG H, FERLAY J, SIEGEL RL, et al. Global Cancer Statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021,71(3):209-249.

GAO W, GUO Z, SHU S, et al. The application effect of percutaneous cryoablation for the stage IIIB/IV advanced non-small-cell lung cancer after the failure of chemoradiotherapy[J]. Asian J Surg, 2018,41(6):530-536.

高梦宇, 周志刚, 王猛,等. 射频消融和氩氦刀冷冻消融治疗Ⅰ期非小细胞肺癌的安全性和疗效对比研究[J]. 介入放射学杂志,2021,30(10):1010-1014.

周晴, 何瀚夫, 汪晟,等. 氩氦刀冷冻消融治疗晚期肺癌的安全性和有效性评价[J]. 介入放射学杂志,2020,29(1):70-75.

周继尧, 刘德森, 李钟,等. 氩氦刀冷冻治疗在晚期肺部肿瘤中的近期疗效观察[J]. 实用临床医药杂志,2019,23(24):18-19,23.

李龙飞, 柳晨, 曹生亚,等. 氩氦刀冷冻治疗对肺癌患者免疫功能影响及临床意义[J]. 中华肿瘤防治杂志,2021,28(16):1231-1235.

DEHGHANI M, KALANI M, GOLMOGHADDAM H, et al. Aberrant peripheral blood CD4+ CD25+ FOXP3+ regulatory T cells/T helper-17 number is associated with the outcome of patients with lymphoma[J]. Cancer Immunol Immunother, 2020,69(9):1917-1928.

刘海洋,孔英君,李世敏,等.调节性T细胞在肺癌中的研究进展[J].临床肺科杂志,2020,25(3):455-458.

JIANG M, WU C, ZHANG L, et al. FOXP3-based immune risk model for recurrence prediction in small-cell lung cancer at stages I–III[J]. J Immunother Cancer , 2021,9(5):e2339.

林事成, 刘殿娜, 周相男,等. 基于MAPK/ERK信号通路探讨冷冻消融对肺癌小鼠的作用机制[J]. 海南医学院学报,2022,28(6):420-424.

林事成, 刘殿娜, 庄垚雪,等. 冷冻消融治疗对肺腺癌细胞凋亡的影响[J]. 安徽医科大学学报,2022,57(6):963-967.

孙逸凡.基于生信分析方法探讨“寒者热之，热者寒之”指导中医药抗肿瘤的现代科学内涵[D].南京:南京中医药大学,2022.

刘鹏, 彭昭文, 涂轩,等. 肿瘤“绿色治疗”理念下探讨氩氦刀联合中医外治疗法新模式[J]. 医药导报,2021,40(4):482-486.

宋紫临, 胡凯文, 周天,等. “护场”理论指导下氩氦刀治疗晚期肺癌临床研究[J]. 中华中医药杂志,2018,33(10):4768-4770.

胡凯文.肿瘤的“绿色治疗”[J].北京中医药大学学报,2013,20(4):5-7.

YANG S， LIU Y， LI MY， et al. FOXP3 promotes tumor growth and metastasis by activating Wnt/β-catenin signaling pathway and EMT in non-small cell lung cancer［J］. Mol Cancer， 2017，16（1）：124.

SIM GC, LIU C, WANG E, et al. IL2 variant circumvents ICOS+ regulatory T-cell expansion and promotes NK cell activation[J]. Cancer Immunol Res, 2016,4(11):983-994.

谭芷芬, 张宏方, 于鹏龙,等. 调衡方对Lewis肺癌荷瘤小鼠瘤组织与肺组织Foxp3、IL-10、TGF-β表达的影响[J]. 现代中西医结合杂志,2022,31(1):26-33,125.

WANG J, GONG R, ZHAO C, et al. Human FOXP3 and tumour microenvironment[J]. Immunology, 2023,168(2):248-255.

DERYUGINA EI, KIOSSES WB. Intratumoral cancer cell intravasation can occur independent of invasion into the adjacent stroma[J]. Cell Rep, 2017,19(3):601-616.

ZHOU T, CHEN Y, LI L, et al. Non-extended cryoablation could be a new strategy in lung cancer management: An experiment on green fluorescent protein-labeled Lewis lung cancer-bearing mice[J]. Cryobiology, 2015,71(1):40-46.

陈琪.不同消融范围对肺癌患者氩氦刀冷消融术临床疗效影响的研究[D].北京:北京中医药大学,2019.

刘传波, 宋紫临, 姜敏,等. 氩氦刀不同冰球覆盖率对非小细胞肺癌患者生存期影响[J]. 中国临床医生杂志,2018,46(10):1201-1204.

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The Second Clinical Medical College of Beijing University of Chinese Medicine

Department of Endocrinology， Dongfang Hospital， Beijing University of Chinese Medicine

Department of Spleen and Stomach Diseases， Wangjing Hospital， China Academy of Chinese Medical Sciences， Beijing Key Laboratory of TCM Diagnosis and Treatment of Functional Gastrointestinal Diseases

Institute of Clinical Basic Medicine of Traditional Chinese Medicine， China Academy of Chinese Medical Sciences

Guang'anmen Hospital， China Academy of Chinese Medical Sciences

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