Objective,2,To study the effect of Shumai Capsule on the co-culture model of vascular endothelial cells（VECs）and vascular smooth muscle cells（VSMCs）and its possible mechanism.,Methods,2,In the first experiment， vascular endothelial cells-smooth muscle cells（VV）co-culture model was established，which was divided into five groups：VV-control group，VV-FBS（5%）grouup，VV-FBS（10%）group and VV-Shumai capsule（VV-Medicated serum，VV-MS）（5%）group and VV-Shumai Capsule（10%）group.The proliferation and migration ability of VECs and VSMCs were detected by CCK-8 method. Proliferating cell nuclear antigen（PCNA），nitric oxide synthase（eNOs）and vascular endothelial growth factor（VEGF）were detected by immunofluorescence.The protein expression of PPARγ and FABP4 genes were detected by PCR and the gene expression by Western Blot. In the second experiment，the recombinant plasmid FABP4 was constructed， and the overexpressed Fabp4 vascular endothelial cells（of-VECs） were constructed by plasmid transfection， and the co-culture model（OFVV）of FABP4 overexpressed recombinant VECs and VSMCs was established，which was divided into five groups including OFVV-control group，OFVV-MS group，OFVV-InF group，OFVV- MS+InF group，and OFVV- Cil group，CCK-8 method method was used to detect the proliferation and migration ability of OF-VECs and VSMCs. and immunofluorescence to detect PCNA and eNOs. PCR and Western Blot were used to detect the gene and protein expression of PPARγ and FABP4 respectively.,Results,2,In the first experiment，the OD values of VECs in VV-MS（10%）group and VV-MS（5%）group were all higher than those in VV-FBS（10%）group，while those in VSMCs group were all lower than those in VV-FBS（10%）and VV-FBS（5%）group，and the difference was statistically significant（,P,<,0.01）.The expression levels of FAPB4 and PPARγ genes and proteins in VV-MS group were lower than those in VV-FBS group，and the difference was statistically significant（,P,<,0.05）.In the second experiment，The OD value of OF-VECs in OFVV-MS+InF group was higher and the OD value of VSMCs was lower than that of other groups，and the difference was statistically significant（,P,<,0.01）.The expression levels of FAPB4 and PPARγ genes and proteins in OFVV-MS+InF group were higher than those in other groups and the difference was statistically significant（,P,<,0.01）.The expression levels of FAPB4 and PPARγ genes and proteins in OFVV-MS+InF group were higher than those in OFVV-MS group，and the difference was statistically significant（,P,<,0.001）.,Conclusion,2,Shumai Capsule can enhance VECs，reduce the migration and proliferation of VSMCs，improve vascular endothelial function and inhibit restenosis after interventional therapy by regulating the expression of FABP4.