Inhibition of Tubeimoside 1 on proliferation, migration and invasion of triple-negative breast cancer cells through AKT/NF-κB signaling pathway

ZHANG Qin-xiang; WEI Shi-jie; SUN Chang-gang

doi:10.16025/j.1674-1307.2024.01.004

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Inhibition of Tubeimoside 1 on proliferation, migration and invasion of triple-negative breast cancer cells through AKT/NF-κB signaling pathway

更新时间：2024-03-04

- Inhibition of Tubeimoside 1 on proliferation, migration and invasion of triple-negative breast cancer cells through AKT/NF-κB signaling pathway
- Beijing Journal of Traditional Chinese Medicine Vol. 43, Issue 1, Pages: 23-26(2024)
- 作者机构：
  
  1.青岛大学医学部中西医结合中心,青岛 266071
  2.青岛市中医医院(青岛市海慈医院),青岛 266033
  3.潍坊医学院附属潍坊市中医院,潍坊 261041
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2024.01.004
  CLC：
- Published：25 January 2024，
  
  Received：05 December 2023，
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张秦祥，魏仕杰，孙长岗.土贝母苷甲通过AKT/NF-κB信号通路抑制三阴性乳腺癌细胞的增殖、迁徙和侵袭能力［J］.北京中医药，2024，43（1）：23-26.

ZHANG Qin-xiang,WEI Shi-jie,SUN Chang-gang.Inhibition of Tubeimoside 1 on proliferation, migration and invasion of triple-negative breast cancer cells through AKT/NF-κB signaling pathway[J]. Beijing Journal of Traditional Chinese Medicine,2024,43(01):23-26.
张秦祥，魏仕杰，孙长岗.土贝母苷甲通过AKT/NF-κB信号通路抑制三阴性乳腺癌细胞的增殖、迁徙和侵袭能力［J］.北京中医药，2024，43（1）：23-26. DOI： 10.16025/j.1674-1307.2024.01.004.

ZHANG Qin-xiang,WEI Shi-jie,SUN Chang-gang.Inhibition of Tubeimoside 1 on proliferation, migration and invasion of triple-negative breast cancer cells through AKT/NF-κB signaling pathway[J]. Beijing Journal of Traditional Chinese Medicine,2024,43(01):23-26. DOI： 10.16025/j.1674-1307.2024.01.004.

摘要

目的

探究土贝母苷甲（TBMS1）对三阴性乳腺癌（TNBC）细胞系MDA-MB-468的影响，并探求其作用机制。

方法

通过MTT实验测定TBMS1对细胞活性的影响并确定给药浓度，通过EDU细胞增殖实验测定TBMS1对细胞增殖能力的影响，通过细胞划痕实验测定TBMS1对细胞迁徙能力的影响，通过Transwell实验测定TBMS1对细胞侵袭能力的影响，通过Western Blot实验测定TBMS1作用于MDA-MB-468细胞的具体作用机制。

结果

TBMS1对MDA-MB-468细胞的增殖、迁徙和侵袭能力呈剂量依赖性抑制。而Western Blot及挽救实验结果表明：TBMS1通过抑制AKT/NF-κB信号通路发挥作用。

结论

TBMS1作为一种天然药物成分，在TNBC的治疗中显示出潜在的抗肿瘤活性，且具有较低的药物毒性。

Abstract

关键词

土贝母苷甲三阴性乳腺癌MDA-MB-468AKT/NF-κB

Keywords

references

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