Objective

2

To explore the effect and mechanism of panax notoginseng（PN）on methicillin-resistant staphylococcus aureus（MRSA） cell wall.

Methods

2

There were PN treatment group and control group in this study. MRSA in control group was not intervened， while MRSA in PN group was intervened by 2 mg/mLPN for 24 hours. The effects of PN on wall peptidoglycan（PGN）and wall phosphate（LTA）in MRSA cells were detected using ELISA. The effect of PN on the thickness of MRSA cell wall was observed through transmission electron microscopy. Genes related to the efficacy of PN were screened using RNA Seq technology，then these genes were verified by RT-PCR.

Results

2

Compared with the control group，the levels of PGN and LTA in MRSA in PN group were significantly reduced （

P

<

0.05）. In PN group， the number and volume of bacteria in dividing stage increased， the dividing membrane of bacteria was blurred and the cell wall thickness was thin （

P

<

0.05）. There were 552 different genes between the two groups. Compared with the control group， 285 genes in PN group were up-regulated and 267 genes were down-regulated. Biological processes （BP） related to differential genes are mainly concentrated in redox， precursor metabolites and energy production， cellular amino acid catabolism and aerobic respiration， cellular components （CC） are mainly concentrated in cellular anatomical entities， cellular organs and non-membrane organelles， and molecular functions （MF） are mainly concentrated in redox enzyme activity， structural molecular activity and electron transfer activity. The differential genes enriched 85 signal pathways， including fatty acid and lysine degradation. The differential genes MraY， MurD， MurC， FemA， FemB and sgtB are related to MRSA cell wall. Compared with the control group， the expressions of MraY， MurD， FemA and FemB in PN group decreased （

P

<

0.05）， while the expressions of MurC and sgtB increased（

P

<

0.05）.

Conclusion

2

PN exerts the therapeutic effect on MRSA by inhibiting its cell wall which is related to reducing the expression of cell wall-related genes and promoting the degradation of fatty acids and lysine.