Comparison of therapeutic effects of Fructus Aurantii Immaturus, Bran stir-fried and Sand-heated Fructus Aurantii Immaturus on functional dyspepsia rats based on mitophagy PINK1 / Parkin signaling pathway

LIU Ze; ZHANG Shuo; XU Xinyu; ZHAO Ruihua; LUO Rong

doi:10.16025/j.1674-1307.2024.08.007

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Comparison of therapeutic effects of Fructus Aurantii Immaturus, Bran stir-fried and Sand-heated Fructus Aurantii Immaturus on functional dyspepsia rats based on mitophagy PINK1 / Parkin signaling pathway

更新时间：2024-09-19

- Comparison of therapeutic effects of Fructus Aurantii Immaturus, Bran stir-fried and Sand-heated Fructus Aurantii Immaturus on functional dyspepsia rats based on mitophagy PINK1 / Parkin signaling pathway
- Beijing Journal of Traditional Chinese Medicine Vol. 43, Issue 8, Pages: 880-885(2024)
- 作者机构：
  
  首都医科大学中医药学院，北京 100069
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2024.08.007
  CLC：
- Published：25 August 2024，
  
  Received：20 December 2023，
- 稿件说明：
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刘泽，张硕，徐欣雨，等.基于线粒体自噬PINK1/Parkin信号通路的枳实、麸炒枳实和烫枳实对功能性消化不良大鼠治疗作用比较［J］.北京中医药，2024，43（8）：880-885.

LIU Ze,ZHANG Shuo,XU Xinyu,et al.Comparison of therapeutic effects of Fructus Aurantii Immaturus, Bran stir-fried and Sand-heated Fructus Aurantii Immaturus on functional dyspepsia rats based on mitophagy PINK1 / Parkin signaling pathway[J]. Beijing Journal of Traditional Chinese Medicine,2024,43(08):880-885.
刘泽，张硕，徐欣雨，等.基于线粒体自噬PINK1/Parkin信号通路的枳实、麸炒枳实和烫枳实对功能性消化不良大鼠治疗作用比较［J］.北京中医药，2024，43（8）：880-885. DOI： 10.16025/j.1674-1307.2024.08.007.

LIU Ze,ZHANG Shuo,XU Xinyu,et al.Comparison of therapeutic effects of Fructus Aurantii Immaturus, Bran stir-fried and Sand-heated Fructus Aurantii Immaturus on functional dyspepsia rats based on mitophagy PINK1 / Parkin signaling pathway[J]. Beijing Journal of Traditional Chinese Medicine,2024,43(08):880-885. DOI： 10.16025/j.1674-1307.2024.08.007.

摘要

目的

基于线粒体自噬PINK1/Parkin信号通路对比枳实、麸炒枳实和烫枳实对功能性消化不良（FD）大鼠的治疗作用。

方法

将36只大鼠随机分为正常组、模型组、多潘立酮组、枳实组、麸炒枳实组、烫枳实组。除正常组外其他各组采用碘乙酰胺灌胃结合夹尾刺激法制作FD大鼠模型。造模结束后各给药组给予相应药液灌胃，模型组和正常组给予等体积生理盐水灌胃，持续14 d。造模和给药时，记录大鼠饮食量、饮水量和体质量。给药结束后，测定大鼠小肠推进率，苏木-伊红（HE）染色观察胃组织损伤情况，Western blot检测自噬相关蛋白PINK1、Parkin、Beclin-1、p62的表达。

结果

与模型组比较，各给药组小肠推进率提高。在造模期间，与正常组比较，各组大鼠的体质量增长减慢，平均日饮食量降低，模型组大鼠平均日饮水量降低。在给药期间，与模型组比较，多潘立酮组、麸炒枳实组和烫枳实组平均日饮食量增加；与模型组比较，麸炒枳实组PINK1、Parkin蛋白表达增加，烫枳实组p62蛋白表达提高、Beclin-1蛋白表达降低，枳实组Beclin-1蛋白表达降低。

结论

枳实、麸炒枳实和烫枳实治疗FD的作用机制可能与抑制PINK1/Parkin信号通路激活有关，麸炒枳实对FD大鼠的治疗效果优于枳实和烫枳实。

Abstract

Objective

To investigate the therapeutic effects of Aurantii Fructus Immaturus（AFI）， bran stir-fried Aurantii Fructus Immaturus and sand-heated Aurantii Fructus Immaturus on functional dyspepsia based on the mitophagy PINK1 / Parkin signaling pathway.

Methods

36 rats were randomly divided into normal group， model group， domperidone group， AFI group， bran stir-fried AFI group and sand-heated AFI group. In addition to the normal group， the FD rat model was made by iodoacetamide gavage combined with tail clamp stimulation in other groups. After the end of modeling， each drug group was given the corresponding liquid medicine by gavage， while the model group and the normal group were given the same volume of normal saline by gavage for 14 days.During modeling and administration， the diet， water intake and body weight of rats were recorded. After the end of administration， the intestinal propulsion rate of rats was measured. The damage of gastric tissue was observed by HE staining. The expression of autophagy-related proteins PINK1， Parkin， Beclin-1 and p62 were detected by Western blot.

Results

Compared with the model group， the intestinal propulsion rate of each administration group were increased. During the modeling period， compared with the normal group， the weight gain of rats in each group were slowed down， the average daily diet decreased， and the average daily water intake of rats in the model group decreased. During the administration period， compared with the model group， the average daily diet of the domperidone group， the bran stir-fried AFI group and the scalded AFI group increased. Compared with the model group， the expression of PINK1 and Parkin protein increased in the bran stir-fried AFI group， the expression of p62 protein were increased and the expression of Beclin-1 protein decreased in the sand-heated AFI group， and the expression of Beclin-1 protein decreased in the AFI group.

Conclusion

The mechanism of AFI， bran stir-fried AFI and sand-heated AFI in the treatment of functional dyspepsia may be related to the inhibition of PINK1 / Parkin signaling pathway. Bran stir-fried AFI has a better therapeutic effect on functional dyspepsia rats.

关键词

枳实麸炒枳实烫枳实功能性消化不良线粒体自噬PINK1/Parkin信号通路

Keywords

Aurantii Fructus Immaturusbran stir-fried Aurantii Fructus Immaturussand-heated Aurantii Fructus Immaturusfunctional dyspepsiamitochondrial autophagyPINK1 / Parkin signaling pathway

references

国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社，2020:258.

樊玉青,张婷兰,刘艳,等.枳实及其有效成分柚皮苷预防术后肠粘连的作用机制分析[J].中国实验方剂学杂志,2022,28(24):50-57.

辛丹,崔爱灵,张亚楠,等.枳实-白芍对C-IBS大鼠血清胃肠神经递质的影响[J].山东中医药大学学报,2022,46(3):373-378.

张炳鑫.中药炮制品古今演变评述[M].北京:人民卫生出版社，2011:438.

郭海军,林洁,李国成,等.功能性消化不良的动物模型研究[J].中国中西医结合消化杂志,2001,9(3):141-142.

李永吉，彭代银.高等学校中药学类专业实验操作指南[M].北京:中国中医药出版社，2017:87

祝婧,钟凌云,叶喜德,等.枳壳不同炮制品的燥性比较及其对功能性消化不良大鼠胃肠功能的影响[J].中国实验方剂学杂志,2017,23(22):20-26.

李娟娟,王凤云,吕林,等.香砂六君子汤对功能性消化不良脾虚证大鼠胃动力及CRF,UCN2表达的影响[J].中国实验方剂学杂志,2022,28(3):1-7.

NI HM, WILLIAMS JA, DING WX. Mitochondrial dynamics and mitochondrial quality control[J]. Redox Biology, 2015,4:6-13.

赵静怡,郑金粟,曹锐.肝郁脾虚型功能性消化不良中医病证结合模型的研究进展[J].北京中医药,2023,42(10):1156-1160.

王治铭,伍丽萍,孙晓滨,等.功能性消化不良发病机制研究进展[J].医学研究杂志,2021,50(6):161-164.

常雄飞,沈凌宇,张立宏,等.健脾理气方改善功能性消化不良大鼠十二指肠微炎症的作用机制[J].北京中医药,2022,41(9):978-982.

FORD AC, MAHADEVA S, CARBONE MF, et al. Functional dyspepsia[J]. Lancet (London, England), 2020,396(10263):1689-1702.

王香香,王煜姣,李莉,等.基于线粒体自噬及Pink1/Parkin信号通路探讨柴胡疏肝散治疗功能性消化不良大鼠的作用机制[J].中国实验方剂学杂志,2023,29(2):45-51.

ZHANG J, WANG X, WANG F, et al. Xiangsha Liujunzi Decoction improves gastrointestinal motility in functional dyspepsia with spleen deficiency syndrome by restoring mitochondrial quality control homeostasis[J]. Phytomedicine, 2022,105:154374.

曾丽君,凌江红,邓静,等.柴胡疏肝散对功能性消化不良大鼠胃窦肌间Cajal间质细胞自噬的影响[J].时珍国医国药,2017,28(5):1041-1044.

李莉,贾庆玲,王煜姣,等.柴胡疏肝散对功能性消化不良大鼠胃组织线粒体功能及线粒体自噬的影响[J].中国实验方剂学杂志,2021,27(23):26-34.

张静,张宇,唐露露,等.基于Pink1/Parkin通路探讨肝豆汤对Wilson病TX模型小鼠的线粒体自噬的影响[J].中国实验方剂学杂志,2022,28(12):111-118.

VALENTE E M, ABOU-SLEIMAN PM, CAPUTO V, et al. Hereditary early-onset Parkinson’s disease caused by mutations in PINK1[J]. Science, 2004,304(5674):1158-1160.

SHIMURA H, HATTORI N, KUBO S, et al. Immunohistochemical and subcellular localization of Parkin protein: absence of protein in autosomal recessive juvenile parkinsonism patients[J]. Ann Neurol, 1999,45(5):668-672.

RAO JS, ZHANG H, KOBETZ E, et al. Predicting DNA methylation from genetic data lacking racial diversity using shared classified random effects[J]. Genomics, 2021,113(1 Pt 2):1018-1028.

张智豪.Bcl-2、Bad蛋白通过PINK1/Parkin通路介导的线粒体自噬在脂多糖诱导急性肺损伤的调节机制[D].南京:南方医科大学,2020.

WANG JH, BEHRNS KE, LEEUWENBURGH C, et al. Critical role of autophage in ischemia/reperfusion injury to aged livers[J]. Autophagy, 2012,8(1):140-141.

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