Mechanism of Yigong Powder in anemia of chronic disease based on TLR/NF-κB signaling pathway

JIANG Yiling; JI Yuting; ZHENG Qin; SHI ling; ZHANG Aiping; WU Zhihao; XU Haitao; ZHANG Ruifeng; HU Jie; LUO Meihong

doi:10.16025/j.1674-1307.2024.10.005

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Mechanism of Yigong Powder in anemia of chronic disease based on TLR/NF-κB signaling pathway

更新时间：2024-11-26

- Mechanism of Yigong Powder in anemia of chronic disease based on TLR/NF-κB signaling pathway
- Beijing Journal of Traditional Chinese Medicine Vol. 43, Issue 10, Pages: 1100-1105(2024)
- 作者机构：
  
  上海市宝山区中西医结合医院上海中医药大学附属宝山医院，上海 201999
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2024.10.005
  CLC：
- Published：25 October 2024，
  
  Received：24 April 2024，
- 稿件说明：
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姜一陵，季玉婷，郑秦，等.基于TLR/NF-κB信号通路的异功散治疗慢性病贫血的机制研究［J］.北京中医药，2024，43（10）：1100-1105.

JIANG Yiling,JI Yuting,ZHENG Qin,et al.Mechanism of Yigong Powder in anemia of chronic disease based on TLR/NF-κB signaling pathway[J]. Beijing Journal of Traditional Chinese Medicine,2024,43(10):1100-1105.
姜一陵，季玉婷，郑秦，等.基于TLR/NF-κB信号通路的异功散治疗慢性病贫血的机制研究［J］.北京中医药，2024，43（10）：1100-1105. DOI： 10.16025/j.1674-1307.2024.10.005.

JIANG Yiling,JI Yuting,ZHENG Qin,et al.Mechanism of Yigong Powder in anemia of chronic disease based on TLR/NF-κB signaling pathway[J]. Beijing Journal of Traditional Chinese Medicine,2024,43(10):1100-1105. DOI： 10.16025/j.1674-1307.2024.10.005.

摘要

目的

探讨异功散通过TLR/NF-κB信号通路治疗慢性病贫血（ACD）的作用及机制。

方法

将60只C57BL/6小鼠分为空白组、模型组、异功散（YGS）组、促红细胞生成素（EPO）组，建立酵母多糖诱导的脓毒症（ZIGI）炎性贫血小鼠模型，在酵母聚糖A（Zymosan A）注射后的第5天造模成功，分别连续给药7 d，比较各组小鼠体质量、血红蛋白（Hb）、肝组织铁调素（HAMP）mRNA、铁转运蛋白（Fpn）mRNA水平、脾脏指数及肝脏IL-1β、TNF-α、IL-6、IFN-γ等炎症因子水平，比较空白组、模型组、YGS组小鼠肝组织TLR4/NF-κB信号通路TLR4、p65、p-p65蛋白以及MD2及CD14 mRNA表达。

结果

造模成功后，造模小鼠的体质量、Hb均较空白组降低（

0.01）。给药7 d后，给药小鼠的Hb水平均上升（

0.01），YGS组体质量升高（

0.01）。与空白组比较，模型组肝组织HAMP mRNA升高（

0.01），Fpn mRNA降低（

0.01）；与模型组比较，YGS组、EPO组肝组织HAMP mRNA表达下降（

0.01），Fpn mRNA水平升高（

0.01）。与空白组比较，模型组脾脏指数升高（

0.01），肝组织IL-1β、TNF-α、IL-6、IFN-γ水平升高（

0.01）；与模型组比较，YGS组脾脏指数降低（

0.01），肝组织IL-1β、TNF-α、IL-6、IFN-γ水平降低（

0.01）。与空白组比较，模型组肝组织TLR4及p65、p-p65蛋白表达增加（

0.01），MD2、CD14 mRNA表达增加（

0.01）；与模型组比较，YGS组肝组织TLR4及p65、p-p65蛋白表达水平降低（

0.01），MD2、CD14 mRNA表达减少（

0.01）。

结论

异功散可能通过调节TLR/NF-κB通路，下调IL-1β、IL-6、TNF-α、IFN-γ等炎症因子水平，进而缩小肿大的脾脏，有效调控hepcidin-ferroportin轴，改善贫血。

Abstract

关键词

慢性病贫血TLR4/NF-κB信号通路hepcidin-ferroportin轴异功散

Keywords

references

STEINBICKER AU, MUCKENTHALER MU. Out of balance: systemic iron homeostasis in iron-related disorders[J]. Nutrients, 2013,5(8):3034-3061.

STAUDER R, VALENT P, THEURL I. Anemia at older age: etiologies, clinical implications, and management[J]. Blood, 2018,131(5):505-514.

MADU AJ, UGHASORO MD. Anaemia of chronic disease: an in-depth review[J]. Med Prin Pract, 2016, 26 (1): 1-9.

WANG Q, DU F, QIAN ZM, et al. Lipopolysaccharide induces a significant increase in expression of iron regulatory hormone hepcidin in the cortex and substantia nigra in rat brain[J]. Endocrinology, 2008,149(8):3920-3925.

WANG CY, BABITT JL. Hepcidin regulation in the anemia of inflammation[J]. Curr Opin Hematol, 2016,23(3):189-197.

薛城,郑秦,季玉婷,等.异功散联合常规西药治疗慢性病贫血(脾气虚证)的随机、双盲、安慰剂对照临床研究[J].上海中医药杂志,2020,54(1):81-85.

LASOCKI S,MILLOT S, VALÉRIE A, et al. Phlebotomies or erythropoietin injections allow mobilization of iron stores in a mouse model mimicking intensive care anemia[J]. Crit Care Med, 2008, 36(8):2388-2394.

魏伟,吴希美,李元建.药理实验方法学[M].北京:人民卫生出版社,2010:69-72.

张鹤,白宇宁,刘绍能,等.现代医学视角下中医“脾主运化”探析[J].北京中医药,2022,41(2):179-181.

罗梅宏. 从“脾主运化”和“运脾生血”理论探讨慢性病贫血的中医病机和治疗[J]. 中医杂志, 2013, 54(18): 1556-1557.

夏黎,罗梅宏.异功散抗炎机制研究进展[J].山东中医杂志,2023,42(8):898-904.

NEMETH E, TUTTLE MS, POWELSON J, et al. Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization[J]. Science, 2004, 306(5704):2090-2093.

CORNA G, CAMPANA L, PIGNATTI E, et al. Polarization dictates iron handling by inflammatory and alternatively activated macrophages[J]. Haematologica, 2010,95(11):1814-1822.

KIM SJ, SHIN HJ, LEE BJ, et al. The antiinflammatory mechanism of igongsan in mouse peritoneal macrophages via suppression of NF-κB/Caspase-1 activation[J]. Phytother Res, 2014, 28(5):736-744.

TSAO CM, CHEN SJ, SHIH MC, et al. Effects of terbutaline on circulatory failure and organ dysfunction induced by peritonitis in rats[J]. Intensive Care Med, 2010, 36(9):1571-1578.

LIU T, ZHANG L, JOO D, et al. NF-κB signaling in inflammation[J]. STTT, 2017, 2(1):1-9.

LEE YS, KIM YH, JUNG YS,et al. Hepatocyte toll-like receptor 4 mediates lipopolysaccharide-induced hepcidin expression[J]. Exp Mol Med, 2017, 49(12):408.

FITZGERALD KA, KAGAN JC. Toll-like receptors and the control of immunity[J]. Cell, 2020,180(6):1044-1066.

TERRA X, VALLS J, VITRAC X, et al. Grape-seed procyanidins act as antiinflammatory agents in endotoxin-stimulated RAW 264.7 macrophages by inhibiting NFkB signaling pathway[J]. J Agric Food Chem, 2007,55(11):4357-4365.

KUZMICH NN, SIVAK KV, CHUBAREV VN, et al.TLR4 signaling pathway modulators as potential therapeutics in inflammation and sepsis[J]. Vaccines (Basel), 2017,5(4):34.

LIBREGTS SF, GUTIERREZ L, DE BRUIN AM, et al.Chronic IFN-gamma production in mice induces anemia by reducing erythrocyte life span and inhibiting erythropoiesis through an IRF-1/PU.1 axis[J]. Blood, 2011, 118(9):2578-2588.

GANZ T. Anemia of Inflammation[J]. NEJM, 2019, 381(12):1148-1157.

ABREU R, QUINN F, GIRI PK. Role of the hepcidin-ferroportin axis in pathogen-mediated intracellular iron sequestration in human phagocytic cells[J]. Blood Adv, 2018,2(10):1089-1100.

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