Mechanisms of <italic style="font-style: italic">Yixuesheng Capsules</italic> in preventing and treating myelosuppression after chemotherapy

YAN Xiang; LU Hairui; TIAN Shaodan; TIAN Lu; HE Ting; GUO Qing; LIU Kexin; CHEN Xinyi

doi:10.16025/j.1674-1307.2025.07.003

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Mechanisms of Yixuesheng Capsules in preventing and treating myelosuppression after chemotherapy

更新时间：2025-09-11

- Mechanisms of Yixuesheng Capsules in preventing and treating myelosuppression after chemotherapy
- Beijing Journal of Traditional Chinese Medicine Vol. 44, Issue 7, Pages: 827-833(2025)
- 作者机构：
  
  1.北京中医药大学东直门医院血液肿瘤科，北京 100700
  2.宁夏回族医药研究所，银川 750021
  3.内蒙古国际蒙医医院血液病科，呼和浩特 015543
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2025.07.003
  CLC：
- Received：27 April 2025，
  
  Published：25 July 2025
- 稿件说明：
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严香，卢海瑞，田劭丹，等.益血生胶囊防治化疗后骨髓抑制的效应机制研究［J］.北京中医药，2025，44（7）：827-833.

YAN Xiang,LU Hairui,TIAN Shaodan,et al.Mechanisms of Yixuesheng Capsules in preventing and treating myelosuppression after chemotherapy[J]. Beijing Journal of Traditional Chinese Medicine,2025,44(07):827-833.
严香，卢海瑞，田劭丹，等.益血生胶囊防治化疗后骨髓抑制的效应机制研究［J］.北京中医药，2025，44（7）：827-833. DOI： 10.16025/j.1674-1307.2025.07.003.

YAN Xiang,LU Hairui,TIAN Shaodan,et al.Mechanisms of Yixuesheng Capsules in preventing and treating myelosuppression after chemotherapy[J]. Beijing Journal of Traditional Chinese Medicine,2025,44(07):827-833. DOI： 10.16025/j.1674-1307.2025.07.003.

摘要

目的

探究益血生胶囊预防及治疗化疗后骨髓抑制的效应机制。

方法

60只BALB/c雄性小鼠按随机数字表法均分为正常组、模型组、干预A组和干预B组。除正常组外，其余各组小鼠采用环磷酰胺100 mg/kg腹腔注射3 d，建立骨髓抑制小鼠模型。干预A组与造模同步予益血生胶囊灌胃（0.39 g/kg），1次/d，连续给药21 d。造模成功后，正常组和模型组小鼠予生理盐水灌胃（每10 g体质量0.1 mL），干预B组予益血生胶囊灌胃（0.39 g/kg），1次/d，连续给药21 d。干预期间检测不同时间点小鼠动物行为学及外周血象变化，实验结束后观察各组小鼠骨髓细胞形态及有核细胞数，双抗体夹心酶联免疫吸附试验（ABC ELISA）法检测各组造血调节因子水平。

结果

与正常组比较，模型组小鼠精神状态较差，活动量明显减少，被毛灰暗粗糙，四肢变白，体质量减轻（

0.05）。与模型组比较，干预A组和干预B组小鼠精神状态、毛色等明显改善，且用药第7、14天后体质量逐渐增加（

0.05，

0.01）；干预21 d后各组小鼠体质量恢复至正常水平（

0.05）。与正常组比较，模型组小鼠红细胞、血红蛋白、血小板、白细胞水平均低（

0.01）。与模型组比较，干预A组和干预B组红细胞、血红蛋白、血小板水平均高（

0.05，

0.01）。与正常组比较，模型组小鼠骨髓组织大量坏死，骨髓有核细胞明显减少（

0.01）。与模型组比较，干预A组和干预B组小鼠骨髓有核细胞数多（

0.01）。与正常组比较，模型组小鼠骨髓促红细胞生成素（EPO）、促血小板生成素（TPO）、粒巨噬细胞集落刺激因子（GM-CSF）、白细胞介素-11（IL-11）、白细胞介素-3（IL-3）、白细胞介素-6（IL-6）水平低（

0.05，

0.01）。与模型组比较，干预A组小鼠EPO、IL-3、IL-6、IL-11水平高（

0.05，

0.01）。与模型组比较，干预B组小鼠TPO、GM-CSF、IL-11、IL-3水平高（

0.05，

0.01）。

结论

益血生胶囊防治化疗后骨髓抑制，可增加外周血细胞数量，升高骨髓有核细胞数，其效应机制可能与调控造血调节因子水平有关，也进一步证明了“肾主骨生髓化血”理论的科学性和实用性。

Abstract

Objective

To investigate the mechanisms of

Yixuesheng Capsules

in preventing and treating chemotherapy-induced myelosuppression.

Methods

Sixty BALB/c male mice were randomly divided into normal， model， intervention A， and intervention B groups using the random number table method. Except for the normal group， mice in the other groups were intraperitoneally injected with cyclophosphamide （100 mg/kg） for 3 days to establish a myelosuppression model. Mice in the intervention A group were gavaged with

Yixuesheng Capsules

（0.39 g/kg） in parallel with modeling， once daily for 21 days. After successful modeling， mice in the normal and model groups were gavaged with saline （0.1 mL per 10 g body mass）， while those in the intervention B group were gavaged with

Yixuesheng Capsules

（0.39 g/kg）， once daily for 21 days. During the intervention， animal behavior and peripheral blood indices were monitored at different time points. At the end of the experiment， bone marrow cell morphology and nucleated cell counts were observed， and levels of hematopoietic regulatory factors were detected by ABC-ELISA.

Results

Compared with the normal group， mice in the model group showed poorer mental state， significantly reduced activity， gray and rough fur， whitened limbs， and weight loss （

0.05）. Compared with the model group， mice in the intervention A and B groups showed significant improvement in mental state and fur condition， and their body weight gradually increased after 7 and 14 days of treatment （

0.05，

0.01）. After 21 days， body weight in all groups returned to normal levels （

0.05）. Compared with the normal group， red blood cells， hemoglobin， platelets， and leukocytes were significantly decreased in the model group （

0.01）. Compared with the model group， erythrocytes， hemoglobin， and platelets were significantly higher in the intervention A and B groups （

0.05，

0.01）. Compared with the normal group， bone marrow tissue in the model group showed mass

ive necrosis and a significant reduction in nucleated cells （

0.01）. Compared with the model group， bone marrow nucleated cell counts were significantly higher in the intervention A and B groups （

0.01）. Compared with the normal group， levels of erythropoietin （EPO）， thrombopoietin （TPO）， granulocyte-macrophage colony-stimulating factor （GM-CSF）， interleukin-11 （IL-11）， interleukin-3 （IL-3）， and interleukin-6 （IL-6） were significantly decreased in the model group （

0.05，

0.01）. Compared with the model group， levels of EPO， IL-3， IL-6， and IL-11 were significantly increased in the intervention A group （

0.05，

0.01）. Compared with the model group， levels of TPO， GM-CSF， IL-11， and IL-3 were significantly elevated in the intervention B group （

0.05，

0.01）.

Conclusion

Yixuesheng Capsules

can improve the general condition of mice with chemotherapy-induced myelosuppression， increase peripheral blood cell counts， and elevate bone marrow nucleated cell numbers. The mechanisms may be related to the regulation of hematopoietic regulatory factor levels.

关键词

Keywords

references

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⁰

Mechanisms of Yixuesheng Capsules in preventing and treating myelosuppression after chemotherapy

DOI：10.16025/j.1674-1307.2025.07.003

摘要

Abstract

关键词

Keywords

references