Effect of <italic style="font-style: italic">Xinshuai Heji</italic> on PGC-1α/FNDC5 signaling pathway and mitochondrial dynamics in rats with heart failure after myocardial infarction

WU Tong; QIU Shenglei; LI Sinai; SHANG Juju; LI Hongjin; KUANG Songtao; WAN Haitong; DING Zhishan

doi:10.16025/j.1674-1307.2025.11.010

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Effect of Xinshuai Heji on PGC-1α/FNDC5 signaling pathway and mitochondrial dynamics in rats with heart failure after myocardial infarction

更新时间：2026-01-29

- Effect of Xinshuai Heji on PGC-1α/FNDC5 signaling pathway and mitochondrial dynamics in rats with heart failure after myocardial infarction
- Beijing Journal of Traditional Chinese Medicine Vol. 44, Issue 11, Pages: 1427-1432(2025)
- 作者机构：
  
  1.首都医科大学附属北京中医医院心血管科，北京 100010
  2.北京市中医药研究所，北京 100010
  3.浙江中医药大学中医心脑血管病研究院，杭州 310053
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2025.11.010
  CLC：
- Received：26 January 2025，
  
  Published：25 November 2025
- 稿件说明：
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吴彤，仇盛蕾，李思耐，等.心衰合剂对心肌梗死后心力衰竭大鼠PGC-1α/FNDC5信号通路及线粒体动力学的影响［J］.北京中医药，2025，44（11）：1427-1432.

WU Tong,QIU Shenglei,LI Sinai,et al.Effect of Xinshuai Heji on PGC-1α/FNDC5 signaling pathway and mitochondrial dynamics in rats with heart failure after myocardial infarction[J]. Beijing Journal of Traditional Chinese Medicine,2025,44(11):1427-1432.
吴彤，仇盛蕾，李思耐，等.心衰合剂对心肌梗死后心力衰竭大鼠PGC-1α/FNDC5信号通路及线粒体动力学的影响［J］.北京中医药，2025，44（11）：1427-1432. DOI： 10.16025/j.1674-1307.2025.11.010.

WU Tong,QIU Shenglei,LI Sinai,et al.Effect of Xinshuai Heji on PGC-1α/FNDC5 signaling pathway and mitochondrial dynamics in rats with heart failure after myocardial infarction[J]. Beijing Journal of Traditional Chinese Medicine,2025,44(11):1427-1432. DOI： 10.16025/j.1674-1307.2025.11.010.

摘要

目的

探讨心衰合剂（XSHJ）对心肌梗死后心力衰竭（HF）大鼠过氧化物酶体增殖物激活受体γ共激活因子1α/纤维连结蛋白Ⅲ型域包含蛋白5（PGC-1α/FNDC5）信号通路及线粒体动力学的影响。

方法

用随机数字表法将大鼠分为假手术组、模型组、阳性对照组及XSHJ低、中、高剂量组。采用结扎大鼠左冠状动脉前降支法建立急性心肌梗死后HF模型，假手术组仅穿线但不结扎。XSHJ低、中、高剂量组分别给予XSHJ 8.75、17.5、35 mg/mL灌胃，均为2 mL/100 g；模型组、假手术组给予等量0.9%氯化钠溶液灌胃；阳性对照组给予曲美他嗪溶液0.5 mg/mL灌胃，2 mL/100 g。自术后1 d起给药，每天灌胃1次，每周5次，共给药4周。采用超声心动仪检测各组大鼠左室射血分数（LVEF）、左室短轴缩短率（FS），用透射电镜观察心肌细胞内线粒体微观结构，用免疫荧光染色（IHC）法检测心肌组织中PGC-1α、FNDC5表达，蛋白免疫印迹法（WB）检测心肌组织中PGC-1α、FNDC5、OPA、Mfn1、Mfn2、Drp1、Fis1蛋白表达，酶联免疫吸附试验（ELISA）检测血清PGC-1α。

结果

与模型组比较，各给药组LVEF、FS值高（

0.05），心肌细胞内线粒体结构损伤减轻，OPA、Mfn1、Mfn2蛋白表达高，Drp1、Fis1蛋白表达低，PGC-1α、FNDC5蛋白表达高（

0.05），且呈现剂量依赖性。

结论

XSHJ可激活心肌梗死后HF大鼠PGC-1α/FNDC5信号通路，抑制心肌细胞线粒体过度分裂，减轻线粒体结构损伤。

Abstract

Objective

To investigate the effects of

Xinshuai Heji

（XSHJ） on the peroxisome proliferator-activated receptor γ coactivator-1α/fibronectin type III domain-containing protein 5 （PGC-1α/FNDC5） signaling pathway and mitochondrial dynamics in rats with post-myocardial infarction heart failure （HF）.

Methods

Rats were randomly assigned using a random number table into sham operation， model， positive control， and XSHJ low-， medium-， and high-dose groups. The post-myocardial infarction HF model was established by ligation of the left anterior descending coronary artery. Rats in the sham group were only threaded without ligation. The XSHJ low-， medium-， and high-dose groups received XSHJ at 8.75， 17.5， and 35 mg/mL， respectively， by gavage at 2 mL/100 g. The model and sham groups received an equal volume of 0.9% sodium chloride solution， and the positive control group received trimetazidine solution at 0.5 mg/mL， 2 mL/100 g. Administration began 1 day after surgery， once daily， five times per week， for 4 weeks. Echocardiography was used to measure left ventricular ejection fraction （LVEF） and fractional shortening （FS）. Transmission electron microscopy observed mitochondrial ultrastructure in myocardial cells. Immunohistochemistry （IHC） detected PGC-1α and FNDC5 expression in cardiac tissue. Western blot （WB） measured protein levels of PGC-1α， FNDC5， OPA， Mfn1， Mfn2， Drp1， and Fis1 in cardiac tissue. ELISA was used to detect serum PGC-1α levels.

Results

Compared with the model group， LVEF and FS were significantly increased in all treatment groups （

0.05）. Mitochondrial structural damage in myocardial cells was reduced. Expression levels of OPA， Mfn1， and Mfn2 were increased， while Drp1 and Fis1 were decreased. PGC-1α and FNDC5 protein expression was upregulated （

0.05） in a dose-dependent manner.

Conclusion

XSHJ can activate the PGC-1α/FNDC5 signaling pathway in rats with post-myocardial infarction HF， inhibit excessive mitochondrial fission in myocardial cells， and reduce mitochondrial structural damage.

关键词

Keywords

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⁰

Effect of Xinshuai Heji on PGC-1α/FNDC5 signaling pathway and mitochondrial dynamics in rats with heart failure after myocardial infarction

DOI：10.16025/j.1674-1307.2025.11.010

摘要

Abstract

关键词

Keywords

references