Reparative effects and mechanisms of <italic style="font-style: italic">Duhuo Jisheng Decoction</italic> on knee articular cartilage injury in rabbits

JIANG Kaizheng; CHEN Jing; LIU Ye; ZHAO Jiuli; LI Pengyang; LYU Tiancheng; LIU Yuxuan; DENG Bin; YE Chao

doi:10.16025/j.1674-1307.2025.12.009

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Reparative effects and mechanisms of Duhuo Jisheng Decoction on knee articular cartilage injury in rabbits

更新时间：2026-01-24

- Reparative effects and mechanisms of Duhuo Jisheng Decoction on knee articular cartilage injury in rabbits
- Beijing Journal of Traditional Chinese Medicine Vol. 44, Issue 12, Pages: 1553-1558(2025)
- 作者机构：
  
  1.北京中医药大学东直门医院洛阳医院洛阳市中医院骨伤科六病区，洛阳 471000
  2.北京中医药大学第三附属医院治未病中心，北京 100029
  3.中国康复科学所康复信息研究所，北京 100068
  4.北京中医药大学东直门医院骨伤科，北京 100700
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2025.12.009
  CLC：
- Received：11 November 2024，
  
  Published：25 December 2025
- 稿件说明：
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蒋凯正，陈婧，刘叶，等.独活寄生汤对兔膝关节软骨损伤的修复作用及机制研究［J］.北京中医药，2025，44（12）：1553-1558.

JIANG Kaizheng,CHEN Jing,LIU Ye,et al.Reparative effects and mechanisms of Duhuo Jisheng Decoction on knee articular cartilage injury in rabbits[J]. Beijing Journal of Traditional Chinese Medicine,2025,44(12):1553-1558.
蒋凯正，陈婧，刘叶，等.独活寄生汤对兔膝关节软骨损伤的修复作用及机制研究［J］.北京中医药，2025，44（12）：1553-1558. DOI： 10.16025/j.1674-1307.2025.12.009.

JIANG Kaizheng,CHEN Jing,LIU Ye,et al.Reparative effects and mechanisms of Duhuo Jisheng Decoction on knee articular cartilage injury in rabbits[J]. Beijing Journal of Traditional Chinese Medicine,2025,44(12):1553-1558. DOI： 10.16025/j.1674-1307.2025.12.009.

摘要

目的

探究独活寄生汤对兔膝关节软骨损伤的修复作用及机制。

方法

将20只大耳白兔随机分为假手术组、模型组、独活寄生汤低剂量组、独活寄生汤中剂量组、独活寄生汤高剂量组，每组4只。采取环钻构建兔膝关节软骨损伤动物模型，假手术组和模型组给予生理盐水灌胃；独活寄生汤各组给予低、中、高剂量汤剂灌胃，持续12周。于第12周采用Mankin评分评估兔膝关节软骨损伤，定量逆转录聚合酶链反应（qRT-PCR）检测兔膝关节软骨损伤修复组织Ⅱ型胶原mRNA表达，酶联免疫吸附试验（ELISA）法检测关节液血小板反应蛋白解整合素金属肽酶-5（ADAMTS-5）、转化生长因子-β1（TGF-β1）水平，免疫组织化学染色法观察兔膝关节软骨损伤修复组织p-Smad3表达。

结果

与假手术组比较，模型组膝关节Mankin评分高（

0.05）；与模型组比较，独活寄生汤各剂量组膝关节Mankin评分均低（

0.05）；与独活寄生汤低剂量组比较，独活寄生汤中、高剂量组膝关节Mankin评分低（

0.05）。与假手术组比较，模型组膝关节软骨损伤处修复组织中Ⅱ型胶原mRNA表达低（

0.05）；与模型组比较，独活寄生汤中剂量组膝关节软骨损伤处修复组织中Ⅱ型胶原mRNA表达高（

0.05）。与假手术组比较，模型组关节液ADAMTS-5水平高、TGF-β1水平低（

0.05）；与模型组比较，独活寄生汤中、高剂量组关节液ADAMTS-5水平低、TGF-β1水平高（

0.05）；与独活寄生汤低剂量组比较，独活寄生汤中、高剂量组关节液ADAMTS-5水平低、TGF-β1水平高（

0.05）。假手术组关节软骨区域可见均匀分布的p-Smad3蛋白表达；模型组软骨损伤处修复组织未见明显p-Smad3蛋白表达；独活寄生汤低剂量组软骨损伤修复处可见少量p-Smad3蛋白表达，独活寄生汤中、高剂量组软骨损伤修复处可见明显的p-Smad3蛋白表达。

结论

独活寄生汤可促进兔膝关节软骨损伤修复、减轻关节液炎症反应，其作用机制可能与激活TGF-β/Smad信号通路有关。

Abstract

Objective

To investigate the effects and mechanisms of

Duhuo Jisheng Decoction

（DHJSD） on the repair of knee cartilage injury in rabbits.

Methods

Twenty white rabbits were randomly divided into five groups （

=4 each）， including sham surgery group， model group， low-dose DHJSD group， medium-dose DHJSD group， and high-dose DHJSD group. A rabbit knee cartilage injury model was established using a ring drill. The sham surgery group and model group received physiological saline by gavage， while the DHJSD groups received low， medium， or high doses of DHJSD by gavage for 12 weeks. At week 12， the Mankin score was used to evaluate knee cartilage injury. Quantitative reverse transcription PCR （qRT-PCR） was used to detect type Ⅱ collagen mRNA expression in the repair tissue at the cartilage injury site. ELISA was used to measure joint fluid levels of ADAMTS-5 and TGF-β1. Immunohistochemistry was used to observe p-Smad3 expression in the repair tissue.

Results

Compared with the sham surgery group， the model group had significantly higher Mankin scores （

0.05）. Compared with the model group， all DHJSD groups had significantly lower Mankin scores （

0.05）， and the medium- and high-dose groups had lower scores than the low-dose group （

0.05）. Type Ⅱ collagen mRNA expression at the injury site was significantly lower in the model group than in the sham surgery group （

0.05

）， and was significantly higher in the medium-dose DHJSD group compared with the model group （

0.055）. Compared with the sham surgery group， the model group had increased ADAMTS-5 and decreased TGF-β1 levels in joint fluid （

0.05）. Compared with the model group， the medium- and high-dose DHJSD groups showed decreased ADAMTS-5 and increased TGF-β1 levels （

0.05）. Compared with the low-dose group， medium- and high-dose DHJSD groups showed decreased ADAMTS-5 and increased TGF-β1 levels （

0.05）. Immunohistochemistry showed uniform p-Smad3 expression in the sham group. The model group showed little p-Smad3 expression at the cartilage injury site. The low-dose group showed sparse expression， while medium- and high-dose groups showed pronounced p-Smad3 expression at the repair site.

Conclusion

Duhuo Jisheng Decoction

promotes repair of knee cartilage injury and reduces inflammatory response in joint fluid. Its mechanism may be related to activation of the TGF-β/Smad signaling pathway.

关键词

Keywords

references

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Reparative effects and mechanisms of Duhuo Jisheng Decoction on knee articular cartilage injury in rabbits

DOI：10.16025/j.1674-1307.2025.12.009

摘要

Abstract

关键词

Keywords

references