Protective effects and mechanisms of <italic style="font-style: italic">Huashi Runzao Huoxue Formula</italic> against concanavalin A-induced autoimmune hepatitis in mice

CHEN Erhong; LIU Quanzhong

doi:10.16025/j.1674-1307.2025.12.010

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Protective effects and mechanisms of Huashi Runzao Huoxue Formula against concanavalin A-induced autoimmune hepatitis in mice

更新时间：2026-01-24

- Protective effects and mechanisms of Huashi Runzao Huoxue Formula against concanavalin A-induced autoimmune hepatitis in mice
- Beijing Journal of Traditional Chinese Medicine Vol. 44, Issue 12, Pages: 1559-1565(2025)
- 作者机构：
  
  1.河南中医药大学第一临床医学院，郑州 450000
  2.河南中医药大学第一附属医院脾胃肝胆科，郑州 450000
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2025.12.010
  CLC：
- Received：18 November 2024，
  
  Published：25 December 2025
- 稿件说明：
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陈二红，刘全忠.化湿润燥活血方对刀豆蛋白A诱导自身免疫性肝炎小鼠的保护作用及机制［J］.北京中医药，2025，44（12）：1559-1565.

CHEN Erhong,LIU Quanzhong.Protective effects and mechanisms of Huashi Runzao Huoxue Formula against concanavalin A-induced autoimmune hepatitis in mice[J]. Beijing Journal of Traditional Chinese Medicine,2025,44(12):1559-1565.
陈二红，刘全忠.化湿润燥活血方对刀豆蛋白A诱导自身免疫性肝炎小鼠的保护作用及机制［J］.北京中医药，2025，44（12）：1559-1565. DOI： 10.16025/j.1674-1307.2025.12.010.

CHEN Erhong,LIU Quanzhong.Protective effects and mechanisms of Huashi Runzao Huoxue Formula against concanavalin A-induced autoimmune hepatitis in mice[J]. Beijing Journal of Traditional Chinese Medicine,2025,44(12):1559-1565. DOI： 10.16025/j.1674-1307.2025.12.010.

摘要

目的

探讨化湿润燥活血方对刀豆蛋白A（ConA）诱导自身免疫性肝炎（AIH）小鼠的保护作用及机制。

方法

将40只小鼠随机分为空白组、模型组、受体相互作用蛋白1（RIP1）抑制剂组，及中药低、高剂量组，每组8只。空白组小鼠给予15 mg/kg生理盐水尾静脉注射，其余各组小鼠给予15 mg/kg ConA尾静脉注射建立AIH模型。建模2 h后，空白组、模型组小鼠给予10 mL/kg生理盐水灌胃，中药低、高剂量组小鼠分别给予1.95、3.90 g/kg化湿润燥活血方灌胃，RIP1抑制剂组小鼠给予1 mg/kg RIP1特异性抑制剂（Nec-1）腹腔注射，1次/d，连续10 d。对比各组肝脾形态外观、肝脾指数、肝脏病理学变化、血清肝功能指标、肝组织炎症介质及氧化应激指标、RIP1/受体相互作用蛋白3（RIP3）/混合谱系蛋白激酶样结构域（MLKL）信号通路mRNA和蛋白表达。

结果

与空白组比较，模型组小鼠肝脾指数高（

0.05），肝细胞内可见淤血、坏死及大量炎症细胞浸润等病理改变；血清肝酶、胆红素水平高，白蛋白水平低（

0.05）；肝组织内炎症介质及氧化应激指标水平高（

0.05）；肝组织内RIP1、RIP3、MLKL mRNA及蛋白磷酸化水平高（

0.05）。与模型组比较，中药低、高剂量组及RIP1抑制剂组小鼠肝脾指数低（

0.05），肝脏病理学损害均不同程度改善；血清肝酶水平、肝内炎症介质及氧化应激指标水平低（

0.05）；肝组织内RIP1、RIP3、MLKL mRNA及蛋白磷酸化水平低（

0.05）。

结论

化湿润燥活血方可明显减轻自身免疫性肝炎小鼠肝损伤，其机制可能与阻断氧化应激诱导的RIP1/RIP3/MLKL信号通路有关。

Abstract

Objective

To investigate the protective effects and mechanisms of

Huashi Runzao Huoxue Formula

（HRHF） against concanavalin A （ConA）-induced autoimmune hepatitis （AIH） in mice.

Methods

Forty mice were randomly divided into five groups （

=8 per group）， including blank group， model group， RIP1 inhibitor （Nec-1） group， low-dose HRHF group， and high-dose HRHF group. The blank group received 15 mg/kg physiological saline via tail vein injection， while the other groups were injected with 15 mg/kg ConA to establish the AIH model. Two hours after modeling

， mice in the blank and model groups were given 10 mL/kg saline by gavage； the low- and high-dose HRHF groups received 1.95 g/kg and 3.90 g/kg HRHF by gavage， respectively； the Nec-1 group received 1 mg/kg Nec-1 by intraperitoneal injection. All treatments were administered once daily for 10 consecutive days. Liver and spleen morphology， liver and spleen indices， liver histopathology， serum liver function indicators， liver inflammatory and oxidative stress markers， and mRNA and protein expression of the RIP1/RIP3/MLKL signaling pathway were assessed.

Results

Compared with the blank group， the model group showed increased liver and spleen indices （

0.05）； liver histopathology revealed congestion， necrosis， and extensive inflammatory cell infiltration； serum liver enzymes and bilirubin were elevated； albumin was decreased （

0.05）； hepatic inflammatory and oxidative stress markers were increased （

0.05）； and mRNA and phosphorylated protein levels of RIP1， RIP3， and MLKL were upregulated （

0.05）. Compared with the model group， mice in the low- and high-dose HRHF groups and the Nec-1 group had decreased liver and spleen indices （

0.05）， improved liver histopathology， reduced serum liver enzyme levels， decreased hepatic inflammatory and oxidative stress markers （

0.05）， and downregulated mRNA and phosphorylated protein expression of RIP1， RIP3， and MLKL （

0.05）.

Conclusion

HRHF can significantly attenuate liver injury in ConA-induced AIH mice， and its mechanism may be related to inhibition of programmed necrosis via blocking the oxidative stress-induced RIP1/RIP3/MLKL signaling pathway.

关键词

Keywords

references

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Protective effects and mechanisms of Huashi Runzao Huoxue Formula against concanavalin A-induced autoimmune hepatitis in mice

DOI：10.16025/j.1674-1307.2025.12.010

摘要

Abstract

关键词

Keywords

references