Effects of <italic style="font-style: italic">Yangxue Antai-</italic>containing serum in promoting angiogenesis of endothelial cells and epithelial mesenchymal transformation and decidualization of endometrial cells by inhibiting autophagy

YAO Weijie; YAN Shiqi; FENG Xin

doi:10.16025/j.1674-1307.2026.01.011

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Effects of Yangxue Antai-containing serum in promoting angiogenesis of endothelial cells and epithelial mesenchymal transformation and decidualization of endometrial cells by inhibiting autophagy

更新时间：2026-04-01

- Effects of Yangxue Antai-containing serum in promoting angiogenesis of endothelial cells and epithelial mesenchymal transformation and decidualization of endometrial cells by inhibiting autophagy
- Beijing Journal of Traditional Chinese Medicine Vol. 45, Issue 1, Pages: 66-75(2026)
- 作者机构：
  
  1.首都医科大学附属北京妇产医院北京妇幼保健院药事部，北京 100006
  2.首都医科大学中医药学院中医络病研究北京市重点实验室，北京 100069
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2026.01.011
  CLC：
- Received：02 January 2025，
  
  Published：25 January 2026
- 稿件说明：
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姚伟洁，闫仕祺，冯欣.养血安胎含药血清抑制自噬、促进内皮细胞血管生成与内膜细胞上皮间质转化及蜕膜化的作用研究［J］.北京中医药，2026，45（1）：66-75.

YAO Weijie,YAN Shiqi,FENG Xin.Effects of Yangxue Antai-containing serum in promoting angiogenesis of endothelial cells and epithelial mesenchymal transformation and decidualization of endometrial cells by inhibiting autophagy[J]. Beijing Journal of Traditional Chinese Medicine,2026,45(01):66-75.
姚伟洁，闫仕祺，冯欣.养血安胎含药血清抑制自噬、促进内皮细胞血管生成与内膜细胞上皮间质转化及蜕膜化的作用研究［J］.北京中医药，2026，45（1）：66-75. DOI： 10.16025/j.1674-1307.2026.01.011.

YAO Weijie,YAN Shiqi,FENG Xin.Effects of Yangxue Antai-containing serum in promoting angiogenesis of endothelial cells and epithelial mesenchymal transformation and decidualization of endometrial cells by inhibiting autophagy[J]. Beijing Journal of Traditional Chinese Medicine,2026,45(01):66-75. DOI： 10.16025/j.1674-1307.2026.01.011.

摘要

目的

探讨养血安胎含药血清抑制自噬、促进内皮细胞血管生成与内膜细胞上皮间质转化及蜕膜化的作用机制。

方法

将JEG-3细胞分为空白对照（Control）组、雷帕霉素（Rapa）组、3甲基腺嘌呤（3-MA）组、Rapa+养血安胎（YXAT）组、3-MA+YXAT组，分别使用Rapa或3-MA诱导或抑制人绒毛膜癌细胞（JEG-3细胞）细胞自噬，用养血安胎含药血清进行干预，将JEG-3细胞与人脐静脉内皮细胞（HUVECs）或人子宫内膜腺癌细胞（HEC-1-A细胞）共培养，透射电镜观察JEG-3细胞的超微结构；Western blotting法测定JEG-3细胞中自噬相关蛋白（Beclin1）、p62、微管相关蛋白1轻链3（LC3）Ⅰ、LC3Ⅱ的表达，HUVECs中血管内皮生长因子（VEGF）、血管内皮细胞生长因子受体2（p-VEGFR2）表达，以及HEC-1-A细胞中上皮细胞-间质转化（EMT）相关蛋白E型钙黏蛋白（E-cadherin）、波形蛋白（Vimentin）、N型钙黏蛋白（N-cadherin）、纤维结合素（Fibronectin）的表达，蜕膜化相关蛋白骨形成蛋白2（BMP2）、胰岛素样生长因子结合蛋白1（IGFBP-1）、催乳素（PRL）、同源框基因A10（HOXA10）蛋白表达；细胞划痕实验检测共培养后HUVECs的迁移率；Transwell小室实验检测共培养后HUVECs的迁移和侵袭能力；细胞骨架实验检测共培养后HUVECs细胞骨架重塑及肌动蛋白的聚合形式（F-actin）表达；小管形成实验检测共培养后HUVECs新生血管的管腔长度。

结果

与Control组比较，Rapa组JEG-3细胞p62蛋白表达低（

0.01），Beclin1蛋白表达、LC3Ⅱ/Ⅰ高（

0.01），自噬体和自噬溶酶体数量多；HUVECs的VEGFA、p-VEGFR2蛋白表达低，细胞侵袭和迁移能力低，管腔长度短，F-actin表达低（

0.05，

0.01）；HEC-1-A细胞的E-cadherin蛋白表达高，Vimentin、N-cadherin、Fibronectin、BMP2、HOXA10、PRL和IGFBP-1蛋白表达低（

0.05，

0.01）。与Rapa组比较，Rapa+YXAT组JEG-3细胞p62蛋白表达高（

0.01），Beclin1蛋白表达低（

0.05），LC3Ⅱ/Ⅰ高（

0.01），自噬体数量多，自噬溶酶体数量少；HUVECs的VEGFA、p-VEGFR2蛋白表达高，细胞侵袭和迁移能力高，管腔长度增加，F-actin表达增加（

0.05，

0.01）；HEC-1-A细胞中E-cadherin蛋白表达低，Vimentin、N-cadherin、Fibronectin、BMP2、HOXA10、PRL、IGFBP-1蛋白表达高（

0.05，

0.01）。

结论

养血安胎含药血清通过抑制自噬体和溶酶体的融合，从而抑制自噬，促进内皮细胞的侵袭和迁移，增加F-actin的表达，促进血管生成以及子宫内膜细胞的EMT、蜕膜化。

Abstract

Objective

To investigate the mechanism by which

Yangxue Antai

（YXAT）-containing serum promotes endothelial cell angiogenesis and endometrial cell epithelial-mesenchymal transition （EMT） and decidualization by inhibiting autophagy.

Methods

JEG-3 cells were divided into Control， rapamycin （Rapa）， Rapa + YXAT， 3-methyladenine （3-MA）， and 3-MA + YXAT groups. Autophagy in JEG-3 cells was induced or inhibited using Rapa or 3-MA， followed by intervention with YXAT-containing serum. JEG-3 cells were co-cultured with human umbilical vein endothelial cells （HUVECs） or human endometrial adenocarcinoma cells （HEC-1-A）. Transmission electron microscopy was used to observe the ultrastructure of JEG-3 cells. Western blotting measured the expression of autophagy-related proteins （Beclin1， p62， LC3Ⅰ， LC3Ⅱ） in JEG-3 cells； VEGF and phosphorylated VEGF receptor 2 （p-VEGFR2） in HUVECs； EMT-related proteins （E-cadherin， Vimentin， N-cadherin， Fibronectin） and decidualization-related proteins （BMP2， HOXA10， PRL， IGFBP-1） in HEC-1-A cells. HUVEC migration was assessed by scratch assay. Invasion and migration was assessed by Transwell assay. Cytoskeleton remodeling and F-actin expression was detected by cytoskeleton assay， and lumen length of the new blood vessels was assessed by tube formation assay.

Results

Compared with the Control group， Rapa treatment decreased p62 expression， increased B

eclin1 and LC3Ⅱ/Ⅰ， and increased autophagosome and autophagolysosome numbers in JEG-3 cells （

0.01）. In HUVECs， VEGFA and p-VEGFR2 expression， invasion， migration， tube length， and F-actin expression were decreased （

0.05，

0.01）. In HEC-1-A cells， E-cadherin expression was increased， whereas Vimentin， N-cadherin， Fibronectin， BMP2， HOXA10， PRL， and IGFBP-1 expression were decreased （

0.05，

0.01）. Compared with the Rapa group， Rapa + YXAT treatment increased p62 expression， decreased Beclin1 expression， further increased LC3Ⅱ/Ⅰ， increased autophagosomes， and decreased autophagolysosomes in JEG-3 cells （

0.05，

0.01）. In HUVECs， VEGFA and p-VEGFR2 expression， invasion， migration， tube length， and F-actin expression were increased （

0.05，

0.01）. In HEC-1-A cells， E-cadherin expression was decreased， while Vimentin， N-cadherin， Fibronectin， BMP2， HOXA10， PRL， and IGFBP-1 expression were increased （

0.05，

0.01）.

Conclusion

YXAT-containing serum inhibits autophagy by suppressing autophagosome-lysosome fusion， thereby promoting endothelial cell invasion and migration， enhancing F-actin expression， facilitating angiogenesis， and promoting EMT and decidualization in endometrial cells.

关键词

Keywords

references

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Effects of Yangxue Antai-containing serum in promoting angiogenesis of endothelial cells and epithelial mesenchymal transformation and decidualization of endometrial cells by inhibiting autophagy

DOI：10.16025/j.1674-1307.2026.01.011

摘要

Abstract

关键词

Keywords

references