Effects of <italic style="font-style: italic">Haizao Yuhu Decoction</italic> containing the anti-drug combinations of <italic style="font-style: italic">Sargassum</italic> and different basidiogenetic of <italic style="font-style: italic">Glycyrrhizae Radix et Rhizoma</italic> on lipid metabolism in rats with goiter

LI Muyun; LIAO Wenyong; LI Na; HUO Min; REN Yuna; XIU Linlin; LIU Haiyan; CHEN Shaohong; ZHONG Gansheng

doi:10.16025/j.1674-1307.2026.01.012

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Effects of Haizao Yuhu Decoction containing the anti-drug combinations of Sargassum and different basidiogenetic of Glycyrrhizae Radix et Rhizoma on lipid metabolism in rats with goiter

更新时间：2026-04-01

- Effects of Haizao Yuhu Decoction containing the anti-drug combinations of Sargassum and different basidiogenetic of Glycyrrhizae Radix et Rhizoma on lipid metabolism in rats with goiter
- Beijing Journal of Traditional Chinese Medicine Vol. 45, Issue 1, Pages: 76-82(2026)
- 作者机构：
  
  北京中医药大学中医学院，北京 100029
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2026.01.012
  CLC：
- Received：25 November 2024，
  
  Published：25 January 2026
- 稿件说明：
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李慕云，廖文勇，李娜，等.含海藻与不同基原甘草反药组合的海藻玉壶汤对甲状腺肿大大鼠脂质代谢的影响［J］.北京中医药，2026，45（1）：76-82.

LI Muyun,LIAO Wenyong,LI Na,et al.Effects of Haizao Yuhu Decoction containing the anti-drug combinations of Sargassum and different basidiogenetic of Glycyrrhizae Radix et Rhizoma on lipid metabolism in rats with goiter[J]. Beijing Journal of Traditional Chinese Medicine,2026,45(01):76-82.
李慕云，廖文勇，李娜，等.含海藻与不同基原甘草反药组合的海藻玉壶汤对甲状腺肿大大鼠脂质代谢的影响［J］.北京中医药，2026，45（1）：76-82. DOI： 10.16025/j.1674-1307.2026.01.012.

LI Muyun,LIAO Wenyong,LI Na,et al.Effects of Haizao Yuhu Decoction containing the anti-drug combinations of Sargassum and different basidiogenetic of Glycyrrhizae Radix et Rhizoma on lipid metabolism in rats with goiter[J]. Beijing Journal of Traditional Chinese Medicine,2026,45(01):76-82. DOI： 10.16025/j.1674-1307.2026.01.012.

摘要

目的

观察含海藻与不同基原甘草反药组合的海藻玉壶汤（HYD）对甲状腺肿大模型大鼠脂质代谢及肝脏的影响。

方法

将96只大鼠用随机数字表法分为空白（Control）组、模型（Model）组、阳性药（Euthyrox）组、HYD-乌拉尔甘草（HYD-U）组、HYD-光果甘草（HYD-G）组、HYD-胀果甘草（HYD-I）组6组，各16只。Control组予相应体积去离子水（1 mL/100 g）灌胃；其余各组给予丙硫氧嘧啶溶液1 mL/100 g灌胃，1次/d，连续灌胃14 d复制甲状腺肿大模型。Control组、Model组予去离子水灌胃，Euthyrox组给予Euthyrox溶液2 μg/100 g灌胃，HYD-U组、HYD-G组、HYD-I组分别给予中药汤剂1.206 g/100 g灌胃，共干预28 d。对比各组血脂水平、病理结构变化及实时定量逆转录聚合酶链反应（RT-qPCR）检测的肝脏相关基因表达水平。

结果

与Control组比较，Model组总胆固醇（TC）、甘油三酯（TG）、载脂蛋白B（ApoB）、极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）水平高，载脂蛋白A1（ApoA1）、高密度脂蛋白（HDL）水平低（

0.05）。与Model组比较，Euthyrox组TC、LDL水平低，HDL水平高（

0.05）；HYD-U组TG水平低，ApoA1、ApoB、HDL、VLDL水平高（

0.05）；HYD-G组ApoA1、ApoB、HDL水平高（

0.05）；HYD-I组TG水平低、TC水平高，ApoA1、ApoB、HDL、VLDL、LDL水平高（

0.05）。与Euthyrox组比较，HYD-U组ApoA1、ApoB、VLDL、LDL水平高（

0.05）；HYD-G组TC、ApoA1、ApoB、HDL、LDL水平高（

0.05）；HYD-I组TC、ApoA1、ApoB、HDL、VLDL、LDL水平高（

0.05）。与HYD-U组比较，HYD-G组TG、TC、LDL水平高，ApoB、VLDL水平低（

0.05）；HYD-I组TC、LDL水平高（

0.05）。与HYD-G组比较，HYD-I组TG水平低，ApoB水平高（

0.05）。各给药组肝细胞排列较整齐，肝小叶结构较清晰，中央静脉居中，未见明显炎症细胞浸润，局部肝细胞水肿或小泡状脂肪空泡较Model减少。与Control组比较，Model组肝脏硬脂酰辅酶A去饱和酶（SCD1）、固醇调节元件结合蛋白（SREBP-1c）、过氧化物酶体增殖物激活受体α（PPARα）mRNA表达低（

0.05）。与Model组比较，Euthyrox组肝脏腺苷酸活化蛋白激酶（AMPK）、PPARα mRNA表达高（

0.05）；HYD-U组、HYD-G组、HYD-I组肝脏AMPK、SREBP-1c、PPARα mRNA表达高（

0.05）。与Euthyrox组比较，HYD-U组肝脏SREBP-1c mRNA表达高（

0.05）；HYD-G组肝脏AMPK、SREBP-1c mRNA表达高（

0.05）；HYD-I组肝脏SREBP-1c mRNA表达高（

0.05）。与HYD-U组比较，HYD-G组肝脏AMPK、SREBP-1c mRNA表达高（

0.05）。与HYD-G组比较，HYD-I组肝脏SREBP-1c mRNA表达高（

0.05）。

结论

含海藻与不同基原甘草反药组合的HYD对甲状腺肿大模型大鼠的血脂水平、肝脏脂质代谢功能及肝脏病理结构均有一定影响。

Abstract

Objective

To observe the effects of

Haizao Yuhu Decoction

（HYD） containing anti-drug combinations of

Sargassum

with different basidiogenetic

Glycyrrhizae Radix et Rhizoma

（GR） on lipid metabolism and liver function in goiter model rats.

Methods

Ninety-six rats were randomly assigned using a random number table to six groups： Control， Model， positive drug （Euthyrox）， HYD-Glycyrrhiza uralensis Fisch. （HYD-U）， HYD-G. glabra L. （HYD-G）， and HYD-G. inflata Bat. （HYD-I）， with 16 rats per group. The Control group received deionized water （1 mL/100 g） via gastric gavage. The other groups were given propylthiouracil （PTU， 1 mL/100 g） once daily for 14 days to induce goiter. After modeling， the Control and Model groups continued to receive deionized water， the Euthyrox group received Euthyrox solution （2 μg/100 g）， and the HYD-U， HYD-G， and HYD-I groups received the respective herbal decoctions （1.206 g/100 g） via gastric gavage for 28 days. Blood lipid levels， liver histopathology， and hepatic gene expression （detected by RT-qPCR） were compared among groups.

Results

Compared with the Control group， the Model group showed increased total cholesterol （TC）， triglycerides （TG）， apolipoprotein B （ApoB）， very low-density lipoprotein （VLDL）， and low-density lipoprotein （LDL）， and decreased apolipoprotein A1 （ApoA1） and high-density lipoprotein （HDL） levels （P < 0.05）. Compared with the Model group， the Euthyrox group showed lower TC and LDL， and higher HDL （P < 0.05）； the HYD-U group showed lower TG and higher ApoA1， ApoB， HDL， and VLDL （P < 0.05）； the HYD-G group showed higher ApoA1， ApoB， and HDL （P < 0.05）； the HYD-I group showed lower TG， and higher TC， ApoA1， ApoB， HDL， VLDL， and LDL （P < 0.05）. Compared with the Euthyrox group， HYD-U showed higher ApoA1， ApoB， VLDL， and LDL （P < 0.05）； HYD-G showed higher TC， ApoA1， ApoB， HDL， and LDL （P < 0.05）； HYD-I showed higher TC， ApoA1， ApoB， HDL， VLDL， and LDL （P < 0.05）. Compared with HYD-U， HYD-G had higher TG， TC， and LDL but lower ApoB and VLDL （P < 0.05）， while HYD-I had higher TC and LDL （P < 0.05）. Compared with HYD-G， HYD-I showed lower TG ，and higher ApoB （P < 0.05）. Histologically， hepatocytes in all treatment groups were arranged uniformly， hepatic lobule structures were clear， and central veins were centered. Inflammatory infiltration was minimal， and local hepatocyte edema or vacuolar lipid accumulation was reduced compared with the Model group. Compared with the Control group， the Model group showed decreased hepatic mRNA expression of stearoyl-CoA desaturase 1 （SCD1）， sterol regulatory element-binding protein-1c （SREBP-1c）， and peroxisome proliferator-activated receptor alpha （PPARα）（P < 0.05）. Compared with the Model group， Euthyrox increased AMPK and PPARα expression （P < 0.05）， while HYD-U， HYD-G， and HYD-I increased AMPK， SREBP-1c， and PPARα expression （P < 0.05）. Compared with Euthyrox， HYD-U increased SREBP-1c （P < 0.05）； HYD-G increased AMPK and SREBP-1c （P < 0.05）； HYD-I increased SREBP-1c （P < 0.05）. Compared with HYD-U， HYD-G increased AMPK and SREBP-1c （P < 0.05）， and compared with HYD-G， HYD-I increased SREBP-1c （P < 0.05）.

Conclusion

HYD containing anti-drug combinations of

Sargassum

with different basidiogenetic GR can modulate blood lipid levels， improve hepatic lipid metabolism， and ameliorate liver histopathological changes in goiter model rats.

关键词

Keywords

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Effects of Haizao Yuhu Decoction containing the anti-drug combinations of Sargassum and different basidiogenetic of Glycyrrhizae Radix et Rhizoma on lipid metabolism in rats with goiter

DOI：10.16025/j.1674-1307.2026.01.012

摘要

Abstract

关键词

Keywords

references