Effects and mechanisms of acupuncture at <italic style="font-style: italic">Baihui</italic> (GV20) penetrating <italic style="font-style: italic">Qubin</italic> (GB7) on mitochondrial dynamics in brain tissue of rats with acute intracerebral hemorrhage

OU Yuyang; LI Dan; ZENG Xuehan; LI Yunong; LUO Yaoxing; ZHANG Yixin

doi:10.16025/j.1674-1307.2026.03.013

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Effects and mechanisms of acupuncture at Baihui (GV20) penetrating Qubin (GB7) on mitochondrial dynamics in brain tissue of rats with acute intracerebral hemorrhage

更新时间：2026-06-02

- Effects and mechanisms of acupuncture at Baihui (GV20) penetrating Qubin (GB7) on mitochondrial dynamics in brain tissue of rats with acute intracerebral hemorrhage
- Beijing Journal of Traditional Chinese Medicine Vol. 45, Issue 3, Pages: 358-365(2026)
- 作者机构：
  
  北京中医药大学第三附属医院针灸科，北京 100029
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2026.03.013
  CLC：
- Received：20 December 2024，
  
  Published：25 March 2026
- 稿件说明：
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欧宇阳，李丹，曾学翰，等.针刺百会透曲鬓对急性脑出血大鼠脑组织线粒体动力学的影响及机制研究［J］.北京中医药，2026，45（3）：358-365.

OU Yuyang,LI Dan,ZENG Xuehan,et al.Effects and mechanisms of acupuncture at Baihui (GV20) penetrating Qubin (GB7) on mitochondrial dynamics in brain tissue of rats with acute intracerebral hemorrhage[J]. Beijing Journal of Traditional Chinese Medicine,2026,45(03):358-365.
欧宇阳，李丹，曾学翰，等.针刺百会透曲鬓对急性脑出血大鼠脑组织线粒体动力学的影响及机制研究［J］.北京中医药，2026，45（3）：358-365. DOI： 10.16025/j.1674-1307.2026.03.013.

OU Yuyang,LI Dan,ZENG Xuehan,et al.Effects and mechanisms of acupuncture at Baihui (GV20) penetrating Qubin (GB7) on mitochondrial dynamics in brain tissue of rats with acute intracerebral hemorrhage[J]. Beijing Journal of Traditional Chinese Medicine,2026,45(03):358-365. DOI： 10.16025/j.1674-1307.2026.03.013.

摘要

目的

探讨针刺百会透曲鬓对脑出血（ICH）大鼠线粒体动力学的影响及机制。

方法

将30只大鼠用随机数字表法分为假手术组（A组）、模型组（B组）、针刺组（C组）、针刺+泛素样蛋白4A（UBL4A）敲低阴性对照组（D组）、针刺+UBL4A敲低组（E组），每组6只。其中D组、E组于建模前3 d进行立体定向病毒注射。除A组外，其他各组采用自体血注入法制备ICH-右侧纹状体大鼠模型。造模1 h后，A组、B组仅捆绑，每天1次，每次30 min，连续3 d。C组、D组、E组进行针刺治疗，连续3 d。3 d后处死大鼠，收集脑组织进行后续检测。对比各组Berderson神经功能评分，用苏木精-伊红（HE）染色观察脑组织病理变化，用实时荧光定量逆转录聚合酶链反应（RT-qPCR）检测脑组织UBL4A mRNA表达，用Western Blotting法检测脑组织Ser637位点磷酸化动力蛋白相关蛋白1［p-Drp1（Ser637）］、线粒体分裂蛋白1（Fis1）、线粒体融合蛋白1（Mfn1）蛋白表达。

结果

与A组比较，B组Berderson神经功能评分高（

0.01）；与B组比较，C组评分低（

0.01）；与C组比较，D组神经功能评分差异无统计学意义（

0.05），E组Berderson神经功能评分高（

0.01）。B组大鼠脑组织存在水肿及大量炎症细胞浸润；与B组比较，C组、D组大鼠脑组织水肿减轻，炎症细胞浸润少；E组脑组织水肿、炎症细胞浸润较B组减轻，但较C组、D组重。与A组比较，B组UBL4A mRNA表达低（

0.01）；与B组比较，C组UBL4A mRNA表达低高（

0.01）；与C组比较，D组UBL4A mRNA表达差异无统计学意义（

0.05），E组UBL4A mRNA表达低（

0.01）。与A组比较，B组大鼠脑组织中p-Drp1（Ser637）、Mfn1蛋白表达低，而Fis1蛋白表达高（

0.01）；与B组比较，C组大鼠脑组织中p-Drp1（Ser637）、Mfn1蛋白表达高，而Fis1蛋白表达低（

0.01）；与C组比较，D组大鼠脑组织中p-Drp1（Ser637）、Fis1和Mfn1蛋白表达差异无统计学意义（

0.05）；与C组比较，E组大鼠脑组织中p-Drp1（Ser637）、Mfn1蛋白表达低，而Fis1蛋白表达高（

0.01）。

结论

针刺百会透曲鬓可能通过调控UBL4A表达抑制ICH大鼠线粒体过度分裂，促进线粒体融合，有助于维持线粒体动力学平衡，从而减轻ICH后神经损伤。

Abstract

Objective

To investigate the effects of acupuncture at

Baihui

（GV20） penetrating

Qubin

（GB7） on mitochondrial dynamics in rats with intracerebral hemorrhage （ICH） and its underlying mechanisms.

Methods

Thirty rats were randomly divided into five groups using a random number table： sham operation group （Group A）， model group （Group B）， acupuncture group （Group C）， acupuncture + UBL4A knockdown negative control group （Group D）， and acupuncture + UBL4A knockdown group （Group E）， with 6 rats in each group. Groups D and E received stereotactic viral injections 3 days before modeling. Except for Group A， the ICH model was established in the remaining groups by autologous blood injection into the right striatum. One hour after modeling， Groups A， B， and C were only restrained and received no other intervention， once daily for 30 min for 3 consecutive days. Groups C， D， and E received acupuncture treatment for 3 consecutive days. Rats were sacrificed after 3 days， and brain tissue was collected for further analysis. Neurological function was evaluated using the Bederson score. Hematoxylin-eosin （HE） staining was used to observe histopathological changes in brain tissue. RT-qPCR was performed to detect UBL4A mRNA expression in brain tissue. Western blot was used to detect the expression of phosphorylated dynamin-related protein 1 at Ser637 ［p-Drp1 （Ser637）］， mitochondrial fission protein 1 （Fis1）， and mitochondrial fusion protein 1 （Mfn1）.

Results

Compared with Group A， Bederson scores in Group B were significantly increased （

0.01）. Compared with Group B， Group C showed significantly lower scores （

0.01）. Compared with Group C， there was no significant difference in neurological scores in Group D （P

0.05）， while Group E showed significantly higher scores （

0.01）. Brain tissue in Group B showed edema and extensive inflammatory cell infiltration. Compared with Group B， Groups C and D showed reduced brain edema and fewer inflammatory infiltrates. In Group E， brain edema and inflammatory infiltration were alleviated compared with Group B but were more severe than those in Groups C and D. Compared with Group A， UBL4A mRNA expression in Group B was significantly decreased （

0.01）. Compared with Group B， Group C showed significantly increased UBL4A mRNA expression （

0.01）. Compared with Group C， there was no significant difference in Group D （

0.05）， while Group E showed significantly decreased UBL4A mRNA expression （

0.01）. Compared with Group A， p-Drp1 （Ser637） and Mfn1 protein expression in Group B were significantly decreased， while Fis1 expression was significantly increased （

0.01）. Compared with Group B， Group C showed increased p-Drp1 （Ser637） and Mfn1 expression and decreased Fis1 expression （

0.01）. Compared with Group C， there were no significant differences in p-Drp1 （Ser637）， Fis1， and Mfn1 expression in Group D （

0.05）. Compared with Group C， Group E showed decreased p-Drp1 （Ser637） and Mfn1 expression and increased Fis1 expression （

0.01）.

Conclusion

Acupuncture at

Baihui

（GV20） penetrating

Qubin

（GB7） may regulate UBL4A expression to inhibit excessive mitochondrial fission and promote mitochondrial fusion in ICH rats， thereby maintaining mitochondrial dynamic balance and alleviating neurological injury after ICH.

关键词

Keywords

references

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Effects and mechanisms of acupuncture at Baihui (GV20) penetrating Qubin (GB7) on mitochondrial dynamics in brain tissue of rats with acute intracerebral hemorrhage

DOI：10.16025/j.1674-1307.2026.03.013

摘要

Abstract

关键词

Keywords

references