摘要:Objective To study the curative effect of pressing manipulation on pain and limited function caused by frozen shoulder.Methods 73 patients with frozen shoulder were randomly divided into treatment group(40 cases)and control group(33 cases).The treatment group was treated with pressing manipulation and basic manipulation.The control group was only treated with basic manipulation.Before and after treatment,VAS score,present pain intensity,McGill pain questionnaire and shoulder Constant scores of both groups were observed and analyzed statistically.Results After 30 days of treatment,the treatment group was better than the control group in VAS score,simplified McGill pain questionnaire,and shoulder Constant scores and the differences were statistically significant(P<0.05).Conclusion The curative effect of pressing manipulation combined with basic manipulation for frozen shoulder is better than that of basic manipulation used only.The curative effect is obviously seen and the adverse reactions are less.Therefore,it is a safe and effective treatment for frozen shoulder.
摘要:Objective To observe the clinical curative effect of modified Guilu Erxian Glue in the treatment of liver cirrhotic hypoproteinemia through the analysis of changes in 60 patients with liver cirrhotic hypoproteinemia before and after taking medicine.Methods 60 patients with confirmed hypoproteinemia of liver cirrhosis from March 2013 to April 2014 without any treatment within one month in outpatient were randomly divided into two groups.The changes,such as ALB,of patients 3 months before and after the treatment were observed.Results The symptoms were improved in both groups,and the experimental group was better than the control group,abdominal distension,loss of appetite and ascites were mainly improved in the control group;while,costal pain,edema and ascites mostly improved in the experimental group.Jaundice and lassitude were not improved in both two groups.There were significant differences before and after ALB treatment,and the treatment group was superior to the control group(P<0.05).Conclusion Modified Guilu Erxian Glue may have better effects in improving the comprehensive clinical curative effect and the symptoms of cirrhotic hypoproteinemia. Modified Guilu Erxian Glue can not only elevate ALB level but also improve the blood routine examination of patients.
摘要:Objective To observe the effect of Tangluoning(TLN)on oxidative stress and apoptosis in high glucose incubated Schwann cells with Keap1/Nrf2/Bcl-2 signal pathway as breakthrough point according to the key pathogenesis of oxidative stress in the prevention and treatment of diabetic peripheral neuropathy(DPN).Methods 150 mmol/L glucose incubated Schwann cells(SCs)were used as cell models followed by setting control group,high glucose group,ALA serum group and TLN serum group,and after intervention for 36 hour(h),flow cytometry were used to analyze superoxide radical(ROS)and apoptosis.JC-1 staining was used to observe mitochondrial membrane potential(MMP).High content analysis(HCA)and Western blot analysis were used to assess the changes of the expression of Keap1,Nrf2,HO-1 and γGCS in Keap1/Nrf2 pathway and Bcl-2 mediated apoptosis related protein including Bcl-2,Bax,Cyto C and Caspase-3.Results After incubated with high glucose for 36 h,apoptosis and ROS levels were increased but MMP decreased in high glucose group compared with the control group(P<0.01).Apoptosis and ROS levels were decreased and MMP increased in TLN serum group compared with high glucose group(P<0.05 or P<0.01).The results of HCA and Western bolt analysis showed that Keap1,Nrf2,HO-1 and γGCS were increased in high glucose group compared with the control group(P<0.01 or P<0.05)and Keap1,Nrf2,HO-1 and γGCS further increased in TLN group compared with high glucose group(P<0.01 or P<0.05).Bcl-2,Bax,Cyto C and Caspase-3 were increased in high glucose group compared with control group(P<0.01 or P<0.05)and Bcl-2 expression further increased but Bax,Cyto C and Caspase-3 decreased in the TLN group compared with the high glucose group(P<0.01 or P<0.05).Conclusion TLN can inhibit the oxidative stress and apoptosis of Schwann cells in high glucose environment,and it is related with the up-regulation of protein expression in Keap1/Nrf2 pathway against antioxidative stress,and regulation of the expression of Bcl-2-related apoptotic pathway protein to inhibit cell apoptosis.
摘要:Objective To investigate the effects of Xiaozhong Zhitong Ointment on remodeling and repair after skeletal muscle injury based on mRNA expression mechanism.Methods After rat model of injured gastrocnemius muscle with the method of contusion model were established,Xiaozhong Zhitong Ointment was used on rats of 4 d,7 d,14 d,21 d,quantitative PCR was used to detect MyoD mRNA and expression level of protein after the injury to explore the mechanism of muscle remodeling and repair for gastrocnemius muscle contusion model rat.Results The expression of MyoD mRNA and expression level of protein in the treatment group were significantly higher than that of the control group and model group(P<0.05),which further confirmed the important role of Xiaozhong Zhitong Ointment in remodeling and repair process.MyoD was rise at 7 d after the injury,and it expression had maintained at a high level until 21 d after the injury.Compared with the model group,the expression peak was around 14 d,and then it returned to a general state.Conclusion After the rat gastrocnemius muscle get injured,in the natural repair process,the expression level of myogenic regulatory factors shows a trend of rising and dropping,the period of 7 to 14 days after the injury is in the peak of the expression.The expression level of MyoD shows a rising trend,and the decline trend of expression is still not obvious even at 21 d after the injury,so it is can be seen that Xiaozhong Zhitong Ointment can promote the expression of MyoD.Xiaozhong Zhitong Ointment can promote the expression of MyoD,and adjust the regeneration and repair of skeleton muscle,which suggest that Xiaozhong Zhitong Ointment can play a role of regenerating and repairing skeletal muscle after the injury through gene regulation.