摘要:目的系统评价补肾治疗血管性认知障碍的临床疗效与安全性。方法检索中国知网数据库(CNKI)、中国生物医学文献数据库(CBM)、万方数字化期刊全文数据库、维普中文科技期刊数据库、PubMed、Embase数据库,检索时间从建库至2020年7月。纳入以血管性认知障碍患者为研究对象并接受补肾治疗的随机对照试验。结局指标包括总有效率、简易精神状态检查(MMSE)、日常生活能力量表(ADL)。使用Cochrane开发的Risk of bias tool(RoB2)评估工具进行质量评估,采用RevMan5.2软件进行Meta分析。结果最终纳入15个随机对照试验,共1 180例患者。结果显示,采用补肾治疗血管性认知障碍的总有效率高于西药治疗[OR=3.53,95%CI(2.54,4.90),P<0.01]。MMSE评分改善优于西药治疗[MD=2.87,95%CI(1.99,3.76),P<0.01]。ADL评分方面与西药差异无统计学意义[MD=1.37,95%CI(-1.51,4.24),P=0.35]。所有研究均未发生不良反应。结论补肾治疗血管性认知障碍疗效优于西药治疗。
摘要:Objective To verify the efficacy and mechanism of Foxiang Powder in the treatment of diabetic gastroparesis based on animal model, and to provide new ideas for the treatment of diabetic gastroparesis.Methods After successful modeling, 50 diabetic gastro paresis SD rats were randomly divided into model group, western medicine group, and high, medium and low dose Chinese medicine groups with 10 rats each.The high, medium and low dose Chinese medicine groups were given Foxiang Powder liquid by gavage with the concentration of 27.5, 13.75, 6.875 g/(kg·d) respectively, and the western medicine group was given domperidone solution with 3.5 mg/(kg·d), the normal group and the model group were given an equal volume of normal saline by gavage all for 8 weeks.The differences in blood glucose, motilin, small intestine propulsion rate and stomach tissue pathological structure were compared among the groups.Results The blood glucose of the normal group was significantly lower than that of the western medicine group, the model group and all the Chinese medicine groups(P<0.05); compared with the model group, the small intestine propulsion rate of the Chinese medicine medium dose group and the western medicine group were increased(P<0.05); the level of motilin in the western medicine group and the medium dose group of Chinese medicine were increased significantly, and the increase of the motilin level was the most in the medium-dose group.Compared with the pathological structure of stomach tissue in the model group, it was observed under a light microscope that the number of neurons in the mesenteric plexus was increased, the circular muscles and longitudinal muscles were thickened, intercellular distance was broadened, the cytoplasm did not form vacuoles, and there was no telangiectasia in the medium dose group.Conclusion The fact that Foxiang Powder could promote gastric emptying in diabetic gastroparesis rats may be related to the increasing of serum motilin levels, improving of blood glucose metabolism disorders, reducing of the pathological damage to the gastric antrum in DGP rats, and reversing of the cytopathology of gastric tissue layer induced by streptozotocin in diabetic rats.
摘要:Objective To studythe effects of Tangluoning(TLN)on oxidative stress and expression of PI3 K/AKT pathway in dorsal root ganglion neurons(DRGn) under high glucose environment.Methods Sixty male SD rats of 6~8 weeks old of clean grade were randomly divided into high,medium and low-concentrations of TLN groups and normal group, andthe crude drugs selected according to the TLN group prescription were given respectively 5,2.5,1.25 g/(kg·d)and equal volume of distilled water by gavage for the preparation of comparative serum.The dorsal root ganglion(DRG) used to prepare cell suspension was obtained from SD fetal rats.DRGn cells were inoculated into 6-well plate and randomly divided into 5 groups: normal group,high glucose group,high concentration of TLN group,medium concentration of TLN group,low concentration of TLN group.After 24 hours of culture,the level of ROS in DRGn cells was detected by fluorescence probe technology,the content of SOD and MDA in rat dorsal root neurons was detected by enzyme-linked immunosorbent assay; PI3 K/Akt protein expression and its phosphate were detected by immunofluorescence and Western blotting Level.Results Compared with the normal group,ROS and MDA were significantly increased(P<0.01),SOD activity significantly decreased,protein expression of PI3 K,p-PI3 K,p-AKT,and p-PI3 K/PI3 K,p-AKT/AKT ratio significantly decreased in high glucose group(P<0.01);the above indexes could be significantly improved in the high,medium and low concentration of TLN groups,and the best was in the medium concentration of TLN group.Conclusion TLN can improve the oxidative stress level of DRGn cells under high glucose environment,and the mechanism may be related to the activation of PI3 K/AKT pathway.