摘要:Objective To explore the distribution rules of traditional Chinese medicine(TCM)syndromes in patients with chronic kidney disease(CKD)and mild cognitive impairment(MCI)and their relationship with various cognitive functions.Methods The clinical data of 139 outpatients and inpatients from the Department of Nephrology of Guang’anmen Hospital, China Academy of Chinese Medical Sciences from May 2017 to December 2018 and TCM syndromes of them were collected.And the Montreal Cognitive Assessment Scale(MoCA)Chinese version was used to assess the cognitive functions which were compared with TCM syndromes.Results The distribution of TCM syndromes: syndrome of qi and blood deficiency> syndrome of blood stasis obstructing collaterals of brain> syndrome of dampness and turbidity damaging clearness> deficiency of kidney essence.The abstract score and language score of patients with deficiency of qi and blood were the lowest(Z=-2.046, P=0.041; Z=-2.108, P=0.035).Conclusion CKD patients complicated with MCI have the highest proportion of deficiency of both qi and blood, and patients with this syndrome are more likely to suffer from impairment of abstract function and language function.
摘要:Objective To investigate the effects of Huangqi Tujian Mixture on the proliferation of glomerular mesangial cells in rats under high-glucose condition and its possible mechanism.Methods Male SD rats were randomly divided into two groups,which were respectively given Huangqi Tujian Mixture and distilled water of equal volume in order to prepare serum contained drug and normal rat serum.The glomerular mesangial(HBZY-1) cells of rats were randomly divided into four groups: 5 mmol/L glucose+normal serum group(control group), 25 mmol/L mannitol + normal serum group(D-mannitol group), 30 mmol/L glucose + normal serum group group(HG group) and 30 mmol/L glucose + drug-contained serum group(HG+HQTJM group).After 24 hours, 48 hours and 72 hours of intervention, CCK8 method was used to detect the proliferation activity and western blot method was used to detect the protein expression changes of collagen Ⅳ and fibronectin in HBZY-1 cells in each group.Meanwhile, the mRNA expressions of tumor necrosis factor-α,Interleukin-1β,phosphatase and tensin homologue deleted on chromosome 10 and protein kinase B in HBZY-1 cells were detected by real time PCR, the expression levels of TNF-α and IL-1 β in the supernatant were measured by Elisa.Results Compared with the control group, D-mannitol group had no significant effect on the proliferation of HBZY-1 cells and the expression of various molecules had no statistical significance(P>0.05), while in the HG group, the proliferation activity of HBZY-1 cells at 48 h and 72 h of intervention increased significantly, the protein expression of COLIV and FN increased, the gene expression of TNF-α, IL-1β and Akt increased significantly(P<0.05), and the mRNA expression of PTEN decreased with the difference not statistically significant(P>0.05), the expression of TNF-α and IL-1β protein in the supernatant of HG group significantly increased(P<0.05).Compared with the HG group, the HG+HQTJM group could significantly inhibit the proliferation of HBZY-1 cells(P<0.05)and the expression of COLIV and fibronectin in mesangial cells after 72 hours’ intervention;the mRNA expression of TNF-α in drug contained serum group was significantly lower than that in HG group after 24 hours’ intervention(P<0.05), and the mRNA expression of IL-1β was obviously lower than that of HG group after 48 hours’ and 72 hours’ intervention(P<0.05).In HBZY-1 cells, the PTEN gene expression significantly increased, Akt gene expression decreased, and TNF-α and IL-1β protein expression in cell culture supernatant increased at three time points of intervention(P<0.05).Conclusion Huangqi Tujian Mixture can significantly reduce the proliferation activity of HBZY-1 cells and down-regulate the protein expression of COLIV, FN in mesangial cells under high-glucose condition, which may be related to the activation of PTEN and the inhibition of inflammation mediated by PI3 K/AKT pathway.
摘要:Objective To observe the effect of Yiqi Tongluo Formula on the protein expression of Wnt4, β-catenin, and Transforming Growth Factor-β1(TGF-β1) in rats’ kidney with unilateral ureteral obstruction(UUO).Methods Ninety six healthy adult SD rats were randomly divided into sham operation group, model group, low, medium and high dose Yiqi Tongluo Formula groups and losartan group respectively, with 16 rats in each group.Except the sham operation group, unilateral ureteral obstruction renal fibrosis model was established by ligating the left ureter.The sham operation group and model group were given normal saline by gavage, and the low, medium and high dose groups of traditional Chinese medicine and losartan group were given the medicines by gavage according to the corresponding drug concentration; 8 rats in each group were killed at random on the 7 th and 14 th day after modeling, and the blood samples were collected to detect the levels of serum creatinine(SCr) and blood urea nitrogen(BUN).The pathological changes of renal tissue were observed by HE staining and Masson staining, and the expression of Wnt4, β-catenin and TGF-β1 protein in renal tissues were detected by Western blot.Results The kidney tissue of the sham operation group was normal.Compared with the sham operation group at the same time point, renal interstitial fibrosis was found in the model group, SCr and BUN levels were increased, Wnt4, β-Catenin and TGF-β1 protein expression in renal tissues were significantly increased and the differences were statistically significant(P<0.05), and with the longer ureteral obstruction, the renal interstitial fibrosis got worse, the renal function reduced more, Wnt4, β-Catenin and TGF-β1 protein expression in renal tissues significantly increased.Compared with the model group at the same time point, the degree of renal interstitial fibrosis was alleviated, Wnt4, β-Catenin and TGF-β1 protein expression in renal tissues significantly decreased, and the differences were statistically significant(P<0.05) in low, medium an high-dose Yiqi Tongluo Decoction group and the Losartan group.Conclusion Wnt/β-catenin signaling pathway is activated in the renal tissues of UUO rats, which may mediate the transcription of TGF-β1.Yiqi Tongluo Formula may inhibit the activation of Wnt/β-catenin signaling pathway and down-regulate the expression of TGF-β1, thus playing an anti-renal fibrosis role.