最新刊期
期 8 ， 2023
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- 摘要：ObjectiveTo analyze the therapeutic effect of Sangbaipi Decoction on bronchiectasis with phlegm-heat obstructing the lung syndrome.MethodsA total of 62 patients with bronchiectasis with phlegm-heat obstructing the lung syndrome， who visited Longhua Hospital Affiliated to the Shanghai University of Traditional Chinese Medicine from January 2019 to December 2022， were randomly divided into a traditional Chinese medicine （TCM） group（n=31） and a control group（n=31）. The control group received routine western medicine treatment alone， and the TCM，group received western medicine combined with Sangbaipi Decoction Granules for one month. The proportion of acute exacerbation of bronchiectasis within 3 months after treatment， the pulmonary function indicators before and after treatment， and the St. George's Respiratory Questionnaire （SGRQ） before and after treatment were compared between the two groups， and the adverse events and laboratory indicators were recorded simultaneously.ResultsWithin 3 months after treatment， there were 4 cases of acute exacerbation in TCM group and 5 cases in control group， with no statistical significance（P>0.05）. At the end of 3 months after treatment， the SGRQ scores （symptoms， activity， psychological score and total score） in both groups were lower than those before treatment（P<0.05）， and the SGRQ scores in TCM group were higher than those in the control group（P<0.05）.At the end of 3 months after treatment， the pulmonary function parameters （FEV1，FVC，FEV1/FVC） of the two groups were all improved compared with those before treatment， but there was no statistical difference between the two groups（P> 0.05）. Compared with the control group， there was no significant difference in the improvement of pulmonary function indexes in the Chinese medicine group（P>0.05）. The incidence of adverse events in TCM group was 32.3%（10/31）， including 4 cases of diarrhea， 3 cases of abdominal distension and 3 cases of nausea. The incidence of adverse events in the control group was 35.5%（11/31）， including 5 cases of diarrhea， 2 cases of abdominal distension and 4 cases of nausea. There was no significant difference between the two groups （χ2=0.072，P>0.05）.ConclusionConventional western medicine treatment combined with Sangbaipi Decoction can significantly improve the clinical symptoms， activity/endurance， and psychological state inpatients with bronchiectasis of phlegm-heat obstructing the lung syndromewith good safety.关键词：Bronchiectasis;syndrome of phlegm-heat obstructing the lung;Sangbaipi Decoction;syndrome differentiation and treatment
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发布时间：2023-09-18 - 摘要：ObjectiveTo study the drug-resistance sensitization effect of Tanreqing injection （TRQ） combined with ceftazidime （CAZ） on pseudomonas aeruginosa （PA） in vitro.MethodsSensitive strain （ATCC 27853） and multi-drug resistant strains （1508032，1608016，1708116，D1，D2，D5） of PA were selected as experimental strains，and 9 antibiotics recommended by the guidelines for the treatment of PA were selected for study.First， minimum inhibitory concentration （MIC） and fractional inhibitory concentration Index （FICI） were used to select the best antibiotics for combination with TRQ. Then， through growth curve， time-kill curve and genetic stability tests， the sensitization of the best combination to PA resistance was analyzed.ResultsThe combination of TRQ and CAZ was the optimum compatibility for drug-resistance sensitization， which had synergistic and additive effects against sensitive bacteria （ATCC 27853） and multi-resistant strains （1508032， 1608016， 1708116， D1， D2， D5）. The growth curve showed that TRQ combined with CAZ had significant inhibitory effect on the growth of PA. The time-kill curve showed that TRQ combined with CAZ significantly enhanced the killing activity of CAZ against PA. Drug-resistance induction experiments suggested that there were two possible ways of sensitization of TRQ combined with CAZ. Firstly， it could maintain the sensitivity of sensitive strain ATCC 27853 to CAZ and inhibit the development of drug resistance. Secondly， it could restore the sensitivity of clinical drug-resistant strain MDR-PA 1508032 to CAZ and inhibit the development of drug resistance. The genetic stability test indicated that there was no significant difference in drug-resistance sensitization after intervention of combination of TRQ and CAZ during the five generations of empty culture， and the drug-resistance sensitization of TRQ combined with CAZ to PA was stable.ConclusionThe combination of TRQ and CAZ could enhance the killing effect of CAZ on PA， restore the sensitivity of PA to CAZ， and inhibit the drug-resistance development with certain genetic stability.关键词：Tanreqing injection;Ceftazidime;resistance sensitization;pseudomonas aeruginosa
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发布时间：2023-09-18 -
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- 摘要：ObjectiveTo explore the protective effect of TiaoganQuzhi Formula（regulatingliver andreducing fat） on nonalcoholic fatty liver （NAFLD） by microRNA-34a （miR-34a） /silent mating type information regulation 2 homolog-1（SIRT1）/peroxisome proliferator-activated receptor （PPARα） signaling pathway.MethodsForty SD rats were selected and randomly divided into a control group， a model group， a low-dose TiaoganQuzhi group， and a high-dose TiaoganQuzhi group， with ten rats in each group. The control group was fed with normal feed， while other groups were fed with high-fat feed to establish a nonalcoholic fatty liver model. The low and high dose TiaoganQuzhi groups were given 9.045 g/kg and 36.18 g/kg drugs respectively after modeling， with the volume of 1 mL/100 g， and the normal group and model group were given same volume of distilled water. Rats were gavaged once a day for 8 weeks. The pathological changes of liver hematoxylin-eosin（HE）， blood lipids and liver function indicators in each group were compared， and miR-34a， SIRT1 and PPARα gene expression in liver tissueweredetected by reverse transcription-polymerase chain reaction （RT-PCR） method.ResultsAfter modeling， the control group showed good mental health， sensitive response and generally good condition. Other groups showed symptoms such as mental exhaustion and decreased activity. After intervention， the general state of treatment group rats were improved. Liver of the control group was shiny and ruddy， without obvious congestion or edema by visual observation. HE showed regular arrangement of liver cells， and liver cord was clear. The model group showed liver tissue pale with congestion and edema in visible， and liver HE showed disordered arrangement of liver cells with fatty vacuolar degeneration. The treatment group showed improvement in liver observation. Compared with the control group， the model group had higher levels of total cholesterol （TC）， triglyceride （TG）， low-density lipoprotein cholesterol （LDL-C） （P<0.01）， while lower levels of high-density lipoprotein cholesterol （HDL-C）（P<0.01）. Compared with the model group， the levels of TC， TG， LDL-C in each TiaoganQuzhi dose group of were low， and the levels of HDL-C were high. The above indicators in TiaoganQuzhi high-dose group of were statistically significant compared with the model group （P<0.05）. Compared with the control group， the levels of alanine transaminase （ALT）and aspartate transaminase （AST） in model group were higher （P<0.01）. Compared with the model group， the levels of ALT and AST in each TiaoganQuzhi dose group were lower， and the above indicators in the TiaoganQuzhi high-dose group had statistical significance compared with the model group （P<0.05）. Compared with the control group， the model group showed higher expression of miR-34a （P<0.05） and lower expression of SIRT1 and PPARα genes （P<0.05）. Compared with the model group， the expression of miR-34a was lower in each TiaoganQuzhi dose group， while the expression of SIRT1 and PPARα genes was higher. Moreover， there was a statistically significant difference in the above indicators between the TiaoganQuzhi high-dose group and the model group （P<0.05）.ConclusionTiaoganQuzhi Formula can reduce miR-34a expression， increase SIRT1/PPARα expression， regulate blood lipids and liver function， and improve liver cell degeneration in NAFLD rats.关键词：Nonalcoholic fatty liver;TiaoganQuzhi Formula;microRNA-34a;silencing regulatory protein 1;peroxisome proliferator-activated receptor;rats
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发布时间：2023-09-18 - 摘要：ObjectiveTo observe the expression of pregnane X receptor （PXR）， nuclear factor κB p65（NF-κBp65）， interleukin -10（IL-10）， tumor necrosis factor -α（TNF-α） and transforming growth factor -β（TGF-β） in colon tissue of rats with ulcerative colitis（UC）of large intestine damp-heat type caused by retention enema with Yuxian Formula.Methods53 rats were randomly divided into 6 groups，that is blank control group，model group，positive control group，low dose treatment group， medium dose treatment groupand high dose treatment group with，8 in blank control groupand 9 in other groups. Except the blank control group，the other groups were established ulcerative colitis models.After successful modeling， the drug was given. The positive control group was given 0.02 g/mL sulfasalazine （SASP） 3 mL enema， and the low， medium and high dosage groups were given 0.09 g/mL， 0.18 g/mL and 0.36 g/mL YuAmaran Decoction 3 mL enema respectively， while the blank control group and model group were given the same volume of normal saline enema，once a day for 14 days.After the experiment finished，the colon tissue was collected，the histological injury score and colonic mucosal injury score of each group were compared. The expressions of PXR protein and NF-κB p65 protein in colon tissue were detected by Western blotting， and the levels of IL-10， TNF-α and TGF-β in colon tissue were detected by ELISA.ResultsThe colon mucosa in blank control group was normal.In the model group， the colonic mucosa was seriously eroded and there were large superficial ulcers. Compared with the model group， the number and area of colonic ulcers in each treatment group were reduced， and the mucosal injury was alleviated.Compared with the blank control group， the scores of colonic mucosal injury， histological injury， NF-κB p65 protein expression， TNF-α and TGF-β levels in the model group were higher， while the expressions of PXR protein and IL-10 in the colon tissue were lower（P<0.01）.Compared with the model group， the scores above， the expression of NF-κB p65 protein， the levels of TNF-α， TGF-β， TNF-α and TGF-β in each treatment group were all low， while the expression of PXR protein and the level of IL-10 were high（P<0.05，P<0.01）. The scores above and the expression of NF-κB p65 protein in the high-dose group were lower than those in the low-dose and middle-dose groups， while the expression of PXR protein and the level of IL-10 were higher than those in the low-dose and middle-dose groups （P<0.05，P<0.01）.There was no significant difference compared with the positive control group of SASP group（P>0.05）.ConclusionYuxian Formula retention enema can repair intestinal mucosal injury in UC rats with damp-heat type of large intestine， and regulate PXR/NF-κB signaling pathway and inflammatory factors.关键词：Yuxian Formula;large Intestine damp-heat type;Ulcerative colitis;inflammation;PXR;NF-κBp65;rat
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发布时间：2023-09-18 -
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- 摘要：ObjectiveThe mechanism of ZHANG Hong-chun's experience in treating chronic obstructive pulmonary disease （COPD） in stable period was analyzed by network pharmacology， and the molecular docking was verified.MethodsThe empirical prescription and core drugs of Professor ZHANG Hong-chun in treating COPD patients in stable period were sorted out. The core drugs were collectively referred to as GubenPingchuan （stablizingthe root and soothing asthma）drugs according to their efficacy. The effective components， action targets and disease targets of Guben antiasthmatic drugswere searched and screenedin the database of traditional Chinese medicine and diseases. the software of Cytoscape 3.7.2 was usedto draw the "drugs-compounds-targets-disease" network， and the PPI network， GO function enrichment and KEGG pathway enrichment of drug and disease intersection targets were analyzed to reveal the drug targets and pathways. GenCLiP3， SwissDock， GEO database， CNKI database and PubMed database were used to verify the resultsby literature computer-aided analysis，gene expression profiles and clinical trials.ResultsThe GubenPingchuan drugs include Licorice， Angelicae Sinensis Radix， Pseudostellariae Radix， Farfarae Flos， Cornus Officinalis Sieb Et Zucc. A total of 55 chemical components， 182 targets， 647 COPD related targets and 93 drug disease intersection targets were screened. PPI network analysis showed that ILs， AKT1， VEGFA， MMPs and other target junction nodes were more than others.GO and KEGG enrichment analysis obtained 127 functional items and 141 signal pathways respectively .The results of GenCLiP3 platform's network analysis and literature analysis were consistent with the network pharmacologic analysis.Molecular docking showed that quercetin-AKT1 and stigmasterol -IL-1β had good docking effect. Gene expression profile data showed that IL-1β and AKT1 played a role in the pathogenesis of COPD. There were also many documents in CNKI and PubMed databases to support the analysis results of this study.ConclusionProfessor ZHANG Hong-chun's Guben Pingchuan drugs for COPD patients in stable stage containquercetin， β-sitosterol and other components in core drugs act on IL， AKT1 and other targets， and play a role in the immune， inflammatory response， oxidative stress and other processes of COPD patients by regulating IL-17， TNF and other signal pathways.关键词：Chronic obstructive pulmonary disease;stationary phase;GubenPingchuan drugs;empirical prescription;network pharmacology;molecular mechanism
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发布时间：2023-09-18 -
- 摘要：ObjectiveTo investigate the clinical effect of massage combined with Huoxue Roujin（activating blood circulation and softening the tendon） Ointment on postural torticollis.Methods91 children with postural torticolliswho visited Beijing Children's Hospital from January 2019 to January 2022 were selected and divided into two groups according to treatment methods， that is control group（57 cases） and observation group（34 cases）. The control group was treated with physiotherapy， and the observation group was treated with massage combined with Huoxue Roujin Ointment on the basis of the control group. Both groups were treated fivetimes a week for two weeks.The recovery rate， severity classification of torticollis before and after treatment， passive joint activity of cervical vertebra and surface electromyography （average root mean square （AVG-RMS） of sternocleidomastoid muscle in supine neutral position and maximum root mean square （Max-RMS） of sternocleidomastoid muscle in affected side） were compared between the two groups.ResultsThe recovery rate of observation group was 58.8% （20/34）higher than that of control group （33.3%）（19/57）， the difference was statistically significant （χ2=5.65，P<0.05）. Compared with before treatment， the severity of torticollis in both groups were improved after 2 weeks of treatment（P<0.05）， and the observation group was better than the control group（P<0.05）. Compared with before treatment， the range of motion of passive rotation joint and passive lateral flexion joint in the two groups were increased after treatment for 2 weeks（P<0.01）， and the observation group was higher than the control group（P<0.05）. Compared with before treatment， AVG-RMS in both groups was decreased after 2 weeks of treatment（P<0.01）， and the observation group was lower than the control group（P < 0.05）. Compared with before treatment， the Max-RMS in the observation group was increased after 2 weeks of treatment（P<0.05）， but there was no significant change in the control group after treatment， and there was no significant difference between the two groups after treatment（P>0.05）.ConclusionMassage combined with Huoxue Roujin Ointment can significantly improve the range of motion of neck joint and sternocleidomastoid muscle tension in the treatment of postural torticollis ininfant patients， and the clinical effect is superior to physical therapy.关键词：Infant;postural torticollis;massage;Huoxue Roujin ointment
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发布时间：2023-09-18 -
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