Objective To explore Chinese herbal medicines for oral nursing on detection of sputum culture during ventilator treatment. Methods 141 cases of pneumonia patients using ventilator were divided into control group of 65 cases and experimental group of 76 cases. According to the differentiation of syndromes,the experiment group was given oral nursing with Chinese herbal medicines,for heat syndrome,Yinhua 30 g,Kushen 30 g,and Huangqin 30 g were prescribed and decocted deeply into 200 m L taken two times a day; for cold syndrome,Kushen 30 g,Shechuangzi 30 g,Ganjiang 30 g,and Gancao 30 g were used and deeply decocted into 200 m L. The control group received routine saline f or oral nursing. The positive condition of sputum culture of two groups before and after oral care with invasive ventilator and non-invasive ventilator were observed and compared. Results The rate of positive results of sputum culture before and after the treatment of oral nursing during invasive ventilation and non-invasive ventilation were 38. 46%and 36. 00% respectively in the experimental group,lower than that of the control group which were 100% and 100% respectively.And the differences were statistically significant（ χ2= 31. 179,29. 568; P < 0. 01）. Conclusion The Chinese herbal medicines for oral nursing can reduce the detection rate of sputum culture so as to prevent the occurrence of ventilator-associated pneumonia.