Objective To develop a HPLC method for simultaneous determination of ferulic acid,Senkyunolide I,Senkyunolide H,Senkyunolide A,z-ligustilide,Butylidenephthalide and α-Cyperone in Ligusticum chuanxiong Hort.-Cyperus rotundus L.. Methods The HPLC system consisted of a Kromasil-C18 column（ 4. 6 mm × 250 mm,5 μm）,with the mobile phase of methanol solution（ A）-water solution containing 0. 1% formic acid（ B） for gradient elution. DAD detector was used and the detection wave lengths were 250 nm and286 nm. The flow rate was 1. 0 m L/min and the column temperature was 25 ℃. Results The liner relationships of seven effective components were favorable; the mean rate of recovery of each were 99. 7%,99. 6%,98. 9%,101. 2%,99. 4%,100. 3%,99. 0%,and RSD were 1. 7%,1. 5%,1. 3%,2. 3%,2. 2%,1. 6%,2. 1%; other methodological investigation results followed the requirements. Conclusion This method is simple,rapid and correct,and it can be used for simultaneous determination of seven effective components in Ligusticum chuanxiong Hort.-Cyperus rotundus L.,and meanwhile,provide scientific evidences for overall quality evaluation and control of it.