Objective To observe the effect of Huoxue Jiedu Formula on the expression of LincR-Ccr2-5’AS in submandibular gland of non-obese diabetic （NOD） mice with Sj?gren’s syndrome （SS）. Methods 34 NOD/Ltj mice were randomly divided into model group,control group and Chinese medicine group,and 12 C57BL/6J mice were used as normal group. The normal group and the model group were given distilled water at 10 mL/kg. The control group was given total glucosides of paeony at 234 mg/kg. The Chinese medicine group was treated with Huoxue Jiedu Formula at 28 g/kg,all for 8 weeks. The eyeballs of mice of each group were taken for blood and serum CCL5 levels were detected by ELISA. The submandibular gland tissues were stained by HE,and observed under the microscope and scored. The expressions of LincR-Ccr2-5’AS,CCR5 mRNA and CCL5 mRNA in the submandibular gland were detected by RT-q PCR. Results Serum CCL5 in the model group was higher than that in the normal group （P < 0. 01）. The serum CCL5 level of the control group and Chinese medicine group was lower than that of the model group （P < 0. 01）. The serum CCL5 level of Chinese medicine group was lower than that of the control group （P < 0. 01）. The histological scores of the normal group were lower than those of NOD mice of other groups （P < 0. 01）. There was no significant difference in the histological scores between the groups of NOD mice （P > 0. 05）. The expression of LincR-Ccr2-5’AS in the submandibular gland of Chinese medicine group was lower than that of the model group （P < 0. 01）. The expression of CCL5 mRNA in the submandibular gland of the control group was lower than that of the model group （P < 0. 01）. There was no significant difference in the expression of CCR5 mRNA in the submandibular glands between the two groups （P > 0. 05）. There was a positive correlation between CCR5 mRNA expression and CCL5 mRNA expression in submandibular gland tissue （r = 0. 512,P < 0. 01）. CCR5 mRNA was positively correlated with serum CCL5 level （r = 0. 413,P < 0. 05）. CCL5 mRNA was positively correlated with serum CCL5 （r = 0. 377,P < 0. 05）. Conclusion Huoxue Jiedu Formula can down-regulate the serum CCL5 level in NOD mice and the expression of LincR-Ccr2-5’AS in submandibular gland tissue,and has no significant effect on the expression of CCR5 and CCL5 in submandibular gland.