Objective To investigate the effect of Qingchang Ligan Formula on hepatocyte autophagy in acute liver failure（ALF） of mice induced by D-galactosamine/lipopolysaccharide.Methods Wild type of healthy C57 BL/6 male mice were randomly divided into normal control group,ALF model group,Qingchang Ligan Formula intervention group,and 3-MA autophagy inhibitor intervention group.ALF model group:ALF model was induced by intraperitoneal injection with D-GalN/LPS;Qingchang Ligan Formula intervention group:3 days before D-GalN/LPS,Qingchang Ligan Formula was given with lavage,once a day;3-MA intervention group:3 days before D-GalN/LPS,Qingchang Ligan Formula was given with lavage,once a day;24 h before D-GalN/LPS,3-MA was given through caudal vein.6 h after D-GalN/LPS administration,the liver tissue and serum of each group were collected.Serum transaminase ALT and AST were assessed.Western blot and real-time fluorescent quantitative PCR were used to detect the expression of autophagy in liver tissue.The mortality and liver damage in mice were observed.Results Compared with the normal control group,the level of transaminase was increased and the level of autophagy decreased in the model group.In Qingchang Ligan Formula intervention group,the level of transaminase was significantly lowered than that of the model group,and the level of autophagy was increased,and the difference was statistically significant（P<0.05）.After inhibiting autophagy,the protective effect of Qingchang Ligan Formula was reversed,and the mortality of mice increased and liver damage aggravated.Conclusion Qingchang Ligan Formula can promote the autophagy level of hepatocytes in mice with acute liver failure caused by D-GalN/LPS,and reduce liver cell damage.