Objective To observe the immediate brain sustained effects of acupuncture at Fēngchí（GB 20）by using resting-state functional magnetic resonance imaging（rsfMRI）technique and explore the brain functional basis of acupuncture at GB20 for regulating visual cortex.Methods 11 healthy subjects randomly received acupuncture or Von Frey filaments（control）at left GB20.The stimulation time was 30 s and the frequency was 60 times/min.fMRI scan was conducted before and immediately after stimulation for 6 min.MATLAB-based DPABI4.3 software was used to analyze rsfMRI data,including fALFF,ReHo,DC for local brain activity changes,as well as the seed point bilateral posterior cingulate cortex（PCC）and the ventral medial prefrontal（VMPFC）for whole brain functional connectivity and observe the immediate brain sustained effect and whole brain functional connectivity after acupuncture at GB20.Results Compared with the tactile stimulation of Von Frey filaments,acupuncture at left GB20 could clearly modulate the right cuneus and right precuneus which involved to the visual function（voxel P value<0.01, cluster P value <0.05）.The FC between PCC and inferior parietal lobule（BA7, 39, 40）were enhanced, and the FC of the VMPFC and the right precuneus（BA7）were decreased（voxel P value<0.01, cluster P value<0.05）.Conclusion Acupuncture at GB20 could modulate the visual center and adjacent brain regions,as well as the default network,which may be an important brain function basis for the treatment of eye diseases.