Objective To compare the pharmacological effects of Shenma Yizhi Formula processed with 3 kinds of preparation techniques on learning and memory capability, brain histopathology, blood lipid indexes, oxidative stress indexes, inflammatory factors, cholinergic indexes in anisodamine-induced model rats.Methods 60 SD rats, 30 female and 30 male, were randomly divided into 6 groups with 10 rats in each group.3 Shenma Yizhi Formula groups with 3 different preparation techniques, including No.1 recipe group, No.2 recipe and No.3 recipe, were given the formula with 2.97 g·kg,-1,·d,-1,;the positive control group, donepezil hydrochloride tablet, with 0.45 mg·kg,-1,·d,-1,;the blank group and the model group, the equal amounts of pure water.After 4 weeks of delivery, the racemic anisodamine hydrochloride（2 mg/kg）was intraperitoneal injected separately to all the rats except for the blank group.The learning and memory ability of all rats was tested by Morris water maze.All rats were anesthetized by chloral hydrate.Serum indexes of lipids, superoxide dismutasee（SOD）, glutathione peroxidase（GSH-PX）, malondialdehyde（MDA）, interleukin-1β（IL-1β）, tumor necrosis factor-α（TNF-α）, nitric oxide（NO）, acetylcho1（Ach）and acetylcholinesterase（AchE）were tested, and the pathological morphology of hippocampal CA1 region observed.Results Compared with the model group, TC level of all Shenma Yizhi Formula groups were decreased（P,<,0.05）.SOD activity and NO level of rats in No.1 recipe group were significantly increased（P,<,0.05）.Compared with No.2 recipe group, the passing platform times of rats in No.3 recipe group were significantly increased（P,<,0.05）.Compared with No.2 recipe group and No.3 recipe group, the SOD activity of No.1 recipe group was significantly increased（P,<,0.05）.Compared with No.3 recipe group, the level of TNF-α was decreased in No.1 recipe group and No.2 recipe group, and the level of NO was increased（P,<,0.05）.Conclusion The pharmacological effects of Shenma Yizhi Formula prepared with 3 kinds of techniques are different, and the pharmacological effect indexes of No.1 recipe were better than those of No.2 recipe and No.3 recipe.