Objective To explore the regulation of drugs for nourishing the liver and kidney,activating blood circulation,eliminating wind as well as the whole prescription for melanocyte activity and apoptosis rate,mitochondrial membrane potential and release of apoptosis protein induced by oxidative damage and discuss the action mechanism.Methods B16 melanocytes were used as the subject and oxidative damage model of melanocytes induced by hydrogen peroxide stimulation,then protective effect on melanocytes were observed.The prescription for nourishing liver and kidney were divided intothe following groups:group of nourishing liver and kidney,group of activating blood,group of eliminating wind,and freeze-dried powder solution was adopted to act on cells.MTT colorimetry was used to observe the growth inhibition of melanocytes in each group;flow cytometry was used to analyze DNA content and determine the proportion of apoptotic cells.Mitochondrial membrane potential detection kit（JC-1 method）was used to detect mitochondrial membrane potential changes.Western blot method was used to detect apoptosis protein.Results In the experiments,the whole prescription group of nourishing liver and kidney（200 μg/mL、100 μg/mL）and the group of activating blood（100 μg/mL）can increase the cell activityafter oxidative damage,the whole prescription group is the most obviously seen.The apoptosis rate of cells in the whole prescription group was the lowest,it is more effective than other groups in protecting melanocytes from oxidative damage.Compared with the control group,there was a significant difference between the 50 μg/mL whole prescription group,200 μg/mL group of nourishing liver and kidney,the 50 μg/mL group of nourishing liver and kidney group,and the protein expression of Bcl-2 were increased（P<0.05） and it indicated that the anti-apoptosis effect was more obvious in whole prescription group and group of nourishing liver and kidney.Capase 9,capase3,and CytC models groups had notsignificant statistical differencewith other groups in protein expression.AIF protein in 25 μg/mL group of eliminating wind,12.5 μg/mL group of eliminating wind had statistical significancewith the model group（P<0.05）.50 μg/mL whole prescription group,50 μg/mL group of nourishing liver and kidney group,50 μg/mL group of activating blood,25 μg/mL group of dispelling wind,12.5 μg/mL group of dispelling wind had statistical significance in Bax protein compared with the model group.Except the whole prescription group and group of nourishing kidney and liver,there was statistical significance in Smac protein expression compared between the drug groups and model group（P<0.05）.Conclusion The nourishing liver and kidney recipe and its component recipes can inhibit melanocyte oxidative damage,which may occur through mitochondrial pathway,suggesting that clinical treatment of vitiligo should be combined with enriching blood and promoting blood circulation,nourishing liver and kidney,and dispelling pathogenic wind.