Objective To observe the effect of ligustrazine injection on apoptosis of human lens epithelial cells stimulated by high glucose.Methods The HLEs（human lens epithelial cells,HLEs）were cultured in vitro and the subcultured cell were divided into three groups,namely the normal glucose group（5.5 mmol/L glucose,N）,the high glucose group（25 mmol/L glucose,H）,and high glucose + ligustrazine group（including 0.2 μg/mL of ligustrazine medium,H+L）.Apoptosis rate of lens epithelial cells in 3 groups was detected by TUNEL staining.The proteins expression levels of apoptosis-promoting protein Caspase-3 and inhibition of apoptosis protein Bcl-2 in three groups were detected by Western blot.Results The apoptosis rate of lens epithelial cells was higher in the high glucose group than in the normal control group and the ligustrazine group（P<0.05）,and the difference was not statistically significant between the ligustrazine group and in the normal control group（P>0.05）.The expression of caspase-3 was higher in the high-glucose group than in the normal control group（P< 0.05）,while the expression of caspase-3 was not significantly changed in the ligustrazine group（P>0.05）.The expression of Bcl-2 was down-regulated in the high glucose group（P<0.05）compared with normal control group,and the difference was not statistically significant between the ligustrazine group and the normal control group（P>0.05）.Conclusion Ligustrazine can inhibitthe apoptosis of humman lens epithelial cells induced by high glucose.