Objective To determine the influence of Qiguiyin Granules on the renal function and the relationship between the inflammatory response and autophagy in rats with acute kidney injury（AKI）caused by sepsis.Methods The sepsis model was established by using cecal ligation and puncture（CLP）in SD rats to induce AKI.They were divided into three groups:sham operation group,CLP group,CLP+Qiguiyin group with each group 6 rats.Each group was measured with the following indicators at the monitoring time:1.circulating indicators:blood pressure.2.renal function indicators:serum creatinine（sCr）,insulin-like growth factor binding protein 7（IGFBP7）,tissue matrix metalloproteinase inhibition Agent-2（TIMP2）.3.inflammation indicators:tumor necrosis factor-α（TNF-α）,TOLL-like receptor 4（TLR4）,c-Jun amino terminal kinase（JNK）,mitogen-activated protein kinase（MAPK）.4.autophagy indicators:LC3-I,LC3-II.Statistical analysis was performed using SPSS19.0 software.The mean of each group was tested by variance homogeneity test,and then multiple sets of mean ANOVA tests were performed.Results The blood pressure of the CLP group and the CLP+Qiguiyin group began to decrease at 12 h,but there was no significant change in the sham operation group.There was no significant difference in blood pressure between the CLP group and the CLP+Qiguiyin group.The serum creatinine in the CLP group and in the CLP+Qiguiyin group increased from 12 h,and were significantly different from the sham operation group.After 24 h,the CLP+Qiguiyin group had lower serum creatinine level than the CLP group.The product of IGFBP-7 and TIMP-2 began to increase significantly after 3 hours in the CLP group,while it in the CLP+Qiguiyin group was significantly lower than that in the CLP group.The levels of TNF-α in CLP group and CLP+Qiguiyin group at 3 h,6 h,12 h,24 h and 48 h were higher than those at 0 h.The levels of TNF-α in the CLP group and CLP+Qiguiyin group were higher than in the sham-operated group at 3 h,6 h,12 h,24 h and 48 h.The levels of TNF-α in the CLP+Qiguiyin group was lower than in the CLP group at 3 h,6 h,12 h,24 h and 48 h.TLR4 in the CLP group was higher than that in the sham operation group at 6 h,12 h and 24 h.There was no significant difference between the CLP+Qiguiyin group and the sham operation group at 6 h,but it was significantly different from the CLP group.Compared with the sham operation group,MAPK in the CLP group increased at 6 h,12 h and 24 h,and decreased in the CLP+Qiguiyin group compared with the CLP group.LC3 II/LC3 I in the CLP group was higher than the sham operation group.And at 12 h and 24 h,LC3 II/LC3 I in the CLP+Qiguiyin group was lower than the CLP group.Conclusion Qiguiyin Granules can improve sepsis in AKI.The change of sepsis in AKI is related to the inflammatory response,which is achieved through the TLR4 and MAPK pathways.Sepsis in AKI is also associated with circulatory perfusion,and autophagy is also the pathophysiological mechanism of sepsis in AKI.Although Qiguiyin Granules does not improve the mean arterial pressure,it can reduce autophagy by inhibiting the inflammatory response so as to benefit sepsis in AKI.