Objective To observe the regulative effect of Qingre Lishi Formula on PI3 K/ARK pathway of fatty liver rats with peroxidation damage. Methods 60 rats were randomly divided into 6 groups, including normal control group, high-fat model group, polyene phosphatidyl choline group, Qingre Lishi Formula low-dose, medium-dose, and high-dose group, and they were fed with high fat diet for establishing the model with fatty liver. From week 6, Qingre Lishi Formula groups were given the medicine respectively with 0.3/100 g/day,0.1 g/100 g/day, 0.05/100 g/day,polyene group with 24.6 mg/100 g/day,12 weeks out. Physiological check was given to observe the condition of fatty liver, ELISA was used to detect serum hs-CRP content and ATPase activity, biochemical analyzer to detect blood lipid and liver function indexes in each experimental group of fatty liver. Western-blot was used to detect the expression of PI3 K/ARK in fatty liver cells. Results The content of hs-CRP of high fat model group was increased significantly, compared with the Qingre Lishi Formula high dose group, the difference was statistically significant（P=0.0331）. ATPase in the experimental groups showed decrease of different degree, serum level of ATPase of Qingre high dose group was significantly increased, and compared with hyperlipidemia model group, the difference was statistically significant（P=0.0401）; Western blot experiment results indicated that PI3 K/ARK expression in liver cell of the experimental rats differed significantly, after Qingre Lishi Formula treatment, the expression level of PI3 K/ARK was significantly lowered. Conclusion Qingre Lishi Formula could improve peroxidation damage due to the formation of abnormal metabolism of lipid and promote the recovery of liver function through the regulation of ATPase, hs-CRP and PI3 K/ARK of inflammatory mediators in liver cells from oxidative stress and has certain effect of improving fatty degeneration.