Objective To explore the effect of methods for activating blood circulation and removing blood stasis on autophagy and Aβ42 in hippocampus tissue of rats with chronic cerebral hypoperfusion.Methods The rat models of chronic cerebral hypoperfusion were made by two-step bilateral common carotid artery occlusion（BCCAO）.SD rats were randomly divided into 3 groups: sham group, model group, and NDS group.Cognition（spatial memory）were evaluated by Morris water maze test.The protein expression of Beclin-1,LC3-Ⅱ,p62 and amyloid precursor protein（APP）were detected by Western Blot.Aβ42 level in hippocampus tissue were detected by ELISA.Results Compared with the sham group, the impairment of spatial memory appeared remarkably, the protein expression of Beclin-1,LC3-Ⅱ,p62 were all increased（P<0.05,P<0.01）,while the expression of APP did not change（P>0.05）in model group, and the expression level of Aβ42 was increased in model group（P<0.01）.Compared with the model group, the cognitive impairment in the NDS group was mildly damaged, the protein expression of Beclin-1,LC3-Ⅱ,p62 were all decreased（P<0.01,P<0.05）,and the expression level of Aβ42 was decreased in NDS group（P<0.05）.Conclusion The methods for activating blood circulation and removing blood stasis could decrease Aβ42 by inhibiting neuron autophagy, to improve cognitive impairment induced by chronic cerebral hypoperfusion.