电针对阿尔茨海默病模型小鼠的治疗作用及机制
Therapeutic effect of electroacupuncture on mouse model with Alzheimer's disease and its mechanism
- 北京中医药 2022年41卷第2期 页码：136-140
- 作者机构：
1.河南省中医院针灸科，郑州 450002
2.河南省中医院迎宾路院区心血管科，郑州 450044
- 作者简介：
咸培伟，男，44岁，博士，主治医师。研究方向：针灸治疗脑病及内伤杂病。
- 基金信息：
DOI：10.16025/j.1674-1307.2022.02.006
中图分类号：
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咸培伟，高丽美，杨煜珂.电针对阿尔茨海默病模型小鼠的治疗作用及机制［J］.北京中医药，2022，41（2）：136-140.
XIAN Pei-wei,GAO Li-mei,YANG Yu-ke.Therapeutic effect of electroacupuncture on mouse model with Alzheimer's disease and its mechanism[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(02):136-140.
咸培伟，高丽美，杨煜珂.电针对阿尔茨海默病模型小鼠的治疗作用及机制［J］.北京中医药，2022，41（2）：136-140. DOI： 10.16025/j.1674-1307.2022.02.006.
XIAN Pei-wei,GAO Li-mei,YANG Yu-ke.Therapeutic effect of electroacupuncture on mouse model with Alzheimer's disease and its mechanism[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(02):136-140. DOI： 10.16025/j.1674-1307.2022.02.006.
摘要
目的,2,观察电针对阿尔茨海默病（AD）模型小鼠的治疗作用，并探讨其作用机制。,方法,2,遵循随机原则，将60只SPF级C57BL/6小鼠按性别分层分为正常对照组、AD模型组、电针组，每组20只，雌雄各10只。AD模型组和电针组采用D-半乳糖皮下注射联合亚硝酸钠腹腔注射法，建立AD小鼠模型。于造模后第2天起，电针组接受电针治疗，1次/d，6 d为1个疗程，共治疗2个疗程。其他2组均只抓取不电针。在电针治疗结束后第2天行Morris水迷宫实验，检测各组小鼠的认知能力；Morris水迷宫测试结束后，小鼠禁食水12 h后取材，采用ELISA法检测各组小鼠海马组织中NOD样受体蛋白3（NLRP3）、TNF-α及IL-1β的含量；RT-PCR检测各组小鼠海马组织中NLRP3 mRNA表达。,结果,2,与正常对照组比较，AD模型组逃避潜伏期长，穿越平台次数、目标象限停留时间及平台象限停留路程占总路程百分比少，大脑海马组织NLRP3、TNF-α、IL-1β含量及NLRP3 mRNA表达高（,P,<,0.05）；与AD模型组比较，电针组逃避潜伏期短，穿越平台次数、目标象限停留时间及平台象限停留路程占总路程百分比多，大脑海马组织NLRP3、TNF-α、IL-1β含量及NLRP3 mRNA表达低（,P,<,0.05）。,结论,2,电针可明显改善AD小鼠学习记忆功能损伤症状，其作用机制可能为下调NLRP3表达，从而抑制脑内神经炎症反应。
Abstract
Objective,2,To investigate the effect and the related mechanism of electroacupuncture in mouse model with Alzheimer's disease （AD）.,Methods,2,60 SPF C57BL/6 mice were randomly divided into control group （,n,=20）， AD model group （,n,=20） and electroacupuncture group （,n,=20）. AD model group and electroacupuncture group were established by utilizing D-galactose subcutaneously injection and joint intraperitoneal injection of sodium nitrite. From the 2nd day after modeling，the electroacupuncture group received electroacupuncture treatment once a day with 6 days as 1 course and 2 treatment courses given in total. The other group of rats were only captured without electroacupuncture. On the second day after the electroacupuncture treatment，the Morris water maze test was used to evaluate the cognitive ability in different groups. After Morris water maze test，the mice were deprived food and water for 12 h before sampling， and the content of NLRP3， TNF-α and IL-1 in hippocampal tissues of three groups were detected by ELISA，and the expression levels of NLRP3 mRNA were measured by RT-PCR .,Results,2,The escape incubation period were increased，and the number of times crossing the platform，the duration of stay in the target quadrant and the percentage of stay in the platform quadrant in the total distance were significantly reduced in the AD model group than those in the normal control group （,P,<,0.05）； compared with the AD model group， the escape incubation period were reduced，and the number of times crossing the platform，the duration of stay in the target quadrant and the percentage of stay in the platform quadrant in the total distance were increased in the electroacupuncture groups （,P,<,0.05）； the contents of the NLRP3，TNF-α and IL-1β in the hippocampus were decreased significantly（,P,<,0.05）.,Conclusion,2,Electroacupuncture could significantly reduce the expression levels of NLRP3 AD mice，which can inhibit the neuroinflammatory response in the brain by down-regulating NLRP3，so as to significantly relieve the symptoms of learning and memory impairment in AD mice.
关键词
阿尔茨海默病电针NOD样受体蛋白3炎性小体小鼠
Keywords
Alzheimer's diseaseelectroacupunctureNLRP3inflammasomemouse
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