Objective,2,To study the protective effect of Jiedu Liangxue Formula on D-galactose （D-GalN） combined with lipopolysaccharide （LPS） induced acute liver failure in mice and its mechanism.,Methods,2,30 mice were randomly divided into normal group，model group and Jiedu Liangxue Formula group with 10 mice in each group. Except for normal group，acute liver failure model was induced by intraperitoneal injection of D-GalN（400 mg/kg） and LPS（0. 25 mg/kg） in the other two groups. The Jiedu Liangxue Formula group was gavaged 500 μL Jiedu Liangxue Formula immediately after the injection of the model agent，and the model group and the normal group were gavaged with the same volume of double steamed water. After 5 hours of modeling，the heart blood was collected and liver tissue samples were kept. Liver function （including serum alanine aminotransferase（ALT），aspartate aminotransferase （AST），total bilirubin （TBIL），direct bilirubin （DBIL），albumin （propagated），cholinesterase （CHE）were tested by automatic biochemical analyzer，the liver tissue pathology change was observed by wood grain - eosin（HE） staining，liver cell apoptosis rate was detected by TUNEL，the protein expressions of caspase-9，caspase-3，glycoprotein 2（SGP-2），Bcl-2 and Bax in mitochondrial apoptosis pathway of liver cells were detected by Western blot，and mRNA expressions of SGP-2，Bcl-2 and Bax were detected by reverse transcriptase polymerase chain reaction（PCR）.,Results,2,Compared with normal group，serum levels of ALT，AST，TBIL and DBIL in model group were significantly increased，CHE was significantly decreased（,P,<, 0.05），the liver pathological damage was obvious，apoptosis cells were significantly increased（,P,<,0.05），the expression of SGP-2，Bax and cleaved caspase-9 and caspase-3 were high，and the expression of Bcl-2 was low （,P,<,0.05）；compared with model group，the levels of ALT，AST，TBIL and DBIL in serum of Jiedu Liangxue Formula group were significantly decreased（,P,<,0.05），the pathological damage of liver tissue was mild，and the apoptosis rate of liver cells was low（,P,<,0.05），SGP-2，Bax and cleaved caspase-9 and cleaved caspase-3 were expressed at low level，and Bcl-2 expression was at high level（,P,<,0.05）.,Conclusion,2,Jiedu Liangxue Formula has treatment effect on D-GalN/ LPS-induced acute liver failure in mice，and its mechanism may be related to the inhibition of hepatocyte apoptosis mediated by mitochondrial apoptosis signaling pathway.